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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28235026 - 03/18/23 12:42 PM (10 months, 6 days ago)

Yeah nuda I got from outgrow. It was probably clean direct from lc but I did two agar transfers before sending it to grain.


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InvisibleSoutherner
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: bw86] * 1
    #28235031 - 03/18/23 12:44 PM (10 months, 6 days ago)

Mine is a clone of a wild find. Pretty sure it’s L. nuda but I’m far from a trusted identifier :lol:



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Offlinebw86
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Registered: 11/12/06
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: Southerner]
    #28243200 - 03/23/23 03:32 PM (10 months, 1 day ago)

I did some hot pours over a few sordita plates. The myc has grown a good bit away from the original visible bacteria. Gonna make plates from the leading edge. (sorry for the condensation )


Today at my door step was a free 5# block from :mushroom:Mt. non sponsor.

I guess my explanation warranted a replacement. Good peeps in my book :thumbup:


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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: bw86]
    #28243294 - 03/23/23 04:59 PM (10 months, 1 day ago)

BW86

I’m not familiar with the term “hot pours” are you implying you poured new agar over the surface of the contaminated plate to allow the mycelium to grow through it but not the bacteria? Because that is a pretty interesting concept I haven’t considered but in theory it could work, just never heard of it being done


Update on my end, the nuda grain jars are just about ready to transfer to sawdust, and the new genetics from outgrow will be here tomorrow


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28243322 - 03/23/23 05:12 PM (10 months, 1 day ago)

Yeah there are teks about hot pours I haven't read one in a while and might not have done it the way "its supposed " to be done.

I poured new agar over half the plate. There is still bacteria suppressed under the myc on the first layer. The far edge of this new myc that crawled up and outside the original footprint of the bacteria should be clean.
Or not :shrug: but its worth a try.
I think I will make supplemented sawdust bags for my nuda tomorrow so we are pretty much neck and neck. Although some pretty terrible shit is happing in my life right now. I'm doing myco work to stay distracted but there are other things that will need take precedence.


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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: bw86]
    #28243818 - 03/23/23 10:02 PM (10 months, 19 hours ago)

That’s really cool info I’ll definitely look into hot pouring, it sounds like a useful strategy I can start employing in the future thanks for the knowledge

I might make bags this weekend but it depends how long it takes to rebuild my flowhood


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


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InvisibleSoutherner
Stranger

Registered: 03/25/21
Posts: 580
Loc: South
Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns] * 1
    #28244112 - 03/24/23 06:25 AM (10 months, 11 hours ago)

Have y’all messed with water agar at all?

https://www.shroomery.org/forums/showflat.php/Number/27900393#27900393

Very easy way to get out of bacteria. When cloning I always take tissue to at least one plate of water agar and usually end up using that material. No good for mold but easy enough to tell what’s what and transfer away from it.


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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: Southerner]
    #28244154 - 03/24/23 07:22 AM (10 months, 10 hours ago)

I’ve seen a lot about water agar but personally haven’t used it, sounds like another thing I’ll consider adding to my arsenal, typically I just use antibiotic agar but it doesn’t work on every bacterial infection


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


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Offline7SunsS
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Registered: 05/11/14
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns] * 2
    #28266170 - 04/06/23 10:52 PM (9 months, 17 days ago)

Well good and bad news

The sordida grain jars are almost done and ready to spawn

The nuda on the other hand have been taken over by an obligate anaerobe possibly a clostridium spp. because the top half of the jar closest to the fresh air exchange is healthy and fully colonized, but the bottom half on all 4 nuda jars are bacterial because of the low oxygen environment as it gets further from the mp tape

I still plan on salvaging the top half of each nuda jar and bulking it out to unsuplemented sawdust to get past the bacteria

Also on a positive note I just finished up the new flowhood and it’s pretty sweet

I’ll make an update in my journal if anyone wants to skim my new build using polycarbonate and $40 hepa filters from Lowes

Sorry to derail a bit just excited about the new hood





--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


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Offlinedyel
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Registered: 10/15/21
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28266264 - 04/07/23 01:43 AM (9 months, 17 days ago)

do you work with glass 7suns?


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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: dyel]
    #28266729 - 04/07/23 10:47 AM (9 months, 17 days ago)

Dyel it depends on what you mean, I’m basically a Jack of all trades what did you have in mind? Maybe shoot me a pm


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


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Offlinedyel
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28266969 - 04/07/23 01:55 PM (9 months, 17 days ago)

nothing specific, you make your flow hood from glass (or at least it looks like it) and have a bunch of glass laying around do i thought you might be a glazier (i learned this word today but don't tell anyone guys) :lol:


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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: dyel]
    #28266997 - 04/07/23 02:14 PM (9 months, 17 days ago)

Oh no it’s actually plexiglass lol glass is a bit heavier and to easy to break for something like this, also if you read it again I did mention it was polycarbonate but the laymen terminology is plexiglass lol sorry for the confusion


Back on topic I’m making lipa Tek sawdust for the nuda as I’m typing this will update in a few hrs probably just going to toss it in a few shoe boxes nothing real sterile lol


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28268009 - 04/08/23 12:54 AM (9 months, 16 days ago)

I salvaged the top half of the nuda spawn when I opened it it smelled like sour rot so it’s actually more likely contaminated with bacillus subtillis not a clostridium species,

Either way I’m hoping the bacteria won’t be able to stick around in an unsuplemented sawdust substrate and the nuda mycelium will flourish

Here’s some pics, hopefully they colonize and recover so I can do some indoor experiments

And I did acquire another nuda culture from outgrow.com and will be putting that on grain this weekend as well



--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


Extras: Filter Print Post Top
Offline7SunsS
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Registered: 05/11/14
Posts: 717
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28269446 - 04/09/23 12:10 AM (9 months, 15 days ago)

Very aggressive mycelium even after surviving bacterial contamination it has fuzzed back up in less then 8 hrs when I checked it this morning but it’s definitely not mold,

Still haven’t used the new culture yet from outgrow, been a bit busy with other projects we’re dedicating a decent area to lab space, and putting in a counter top with sink and stove top range for grain prep work space


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


Extras: Filter Print Post Top
Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 20 hours, 53 minutes
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28270123 - 04/09/23 03:21 PM (9 months, 15 days ago)

I have got a lot of work done with other species but just made a couple nuda supplemented hwfp bags this week.
I also finally had a good plate to make a master pint with my sordita but fumbled the transfer. (The lid on my pint wasn't fully unscrewed and I was using a micro-scalple ) :facepalm: but w/e i will make another t3 transfer before making a master pint.


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Offlinekedi
people are strange


Registered: 01/14/23
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: bw86]
    #28270149 - 04/09/23 03:51 PM (9 months, 15 days ago)

i remember reading in one of them ''books'' - blewits need to be cooked, some bad toxins for blood cells or something not just stomach problems and vaguely remember not to eat too many, and maybe get rid of the water leachate somehow.. i used to microwave them with paper towel.. i cant remember exactly .. anyway, need cook them properly apparently


Edited by kedi (04/09/23 03:54 PM)


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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: kedi]
    #28272134 - 04/11/23 01:17 AM (9 months, 13 days ago)

Finally put the outgrow nuda culture on grain, and agar, my sordida look like they will be ready to go in substrate this weekend, not sure what I want to use yet, almost considering trying straw on one of the jars


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


Extras: Filter Print Post Top
InvisibleSoutherner
Stranger

Registered: 03/25/21
Posts: 580
Loc: South
Re: Wood blewit / clitocybe nuda cultivation thread [Re: 7Suns]
    #28272426 - 04/11/23 08:15 AM (9 months, 13 days ago)

Mine are still moving quite slowly. I decided to open this bag up and grab a couple of pieces of myc from this growth that seems to do okay on masters mix. Hopefully narrowing down genetics will get better/faster growth from the clone culture.



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Offline7SunsS
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Re: Wood blewit / clitocybe nuda cultivation thread [Re: Southerner]
    #28272607 - 04/11/23 10:14 AM (9 months, 13 days ago)

Yea if yo can isolate a pheno that moves faster it could help not sure if you would be better off starting with spores or if tissue samples will have enough divergent types to sector from


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber


Extras: Filter Print Post Top
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