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Offlinenakadash1
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Registered: 11/02/22
Posts: 156
Last seen: 1 year, 2 months
How did I contaminate this plate so badly? (pics)
    #28184308 - 02/12/23 04:12 PM (1 year, 10 months ago)

Both plates were inoculated from an agar-agar plate (deep poured, 3-4cm) with minimal surface growth. Both transfers were performed in a SAB. The only thing I can think of is perhaps condensation could have helped spread spores all over the plate?

Even the amount of growth is wildly different for some reason -- both were inoculated 5 days ago, the medium is exactly the same although it is about half the thickness on the larger plate.


*Edit reposted the wrong text -_-









--------------------
#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold

Edited by nakadash1 (02/12/23 04:43 PM)

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Invisiblestubb
Dahg Rastubfari
 User Gallery

Registered: 03/23/19
Posts: 1,447
Loc: Memory Flag
Re: How did I clamtanibate this plot so boodly? (pics) [Re: nakadash1]
    #28184327 - 02/12/23 04:24 PM (1 year, 10 months ago)

See Tic Tac Toe:  https://www.shroomery.org/forums/showflat.php/Number/22601023


OP fixed his post, I swear I'm not just shouting random teks at passersby like the town drunk  :lol:

Edited by stubb (02/12/23 05:46 PM)

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Offlinenakadash1
Stranger
Registered: 11/02/22
Posts: 156
Last seen: 1 year, 2 months
Re: How did I contaminate this plate so badly? (pics) [Re: stubb]
    #28184340 - 02/12/23 04:35 PM (1 year, 10 months ago)

Quote:

stubb said:
See Tic Tac Toe:  https://www.shroomery.org/forums/showflat.php/Number/22601023




I think you meant to reply to my other thread, thank you though! I think I'll try that also. I figure it's a tradeoff between more inoculation points vs contamination risk.


--------------------
#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold

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InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: How did I contaminate this plate so badly? (pics) [Re: nakadash1]
    #28184351 - 02/12/23 04:38 PM (1 year, 10 months ago)

I’m confused. :tongue:

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OfflineLand TroutM
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I'm a teapot User Gallery


Registered: 01/08/18
Posts: 3,180
Last seen: 7 hours, 4 minutes
Trusted Cultivator
Re: How did I contaminate this plate so badly? (pics) [Re: Mycolorado]
    #28184356 - 02/12/23 04:41 PM (1 year, 10 months ago)

Quote:

Mycolorado said:
I’m confused. :tongue:



:whathesaid:

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Offlinenakadash1
Stranger
Registered: 11/02/22
Posts: 156
Last seen: 1 year, 2 months
Re: How did I contaminate this plate so badly? (pics) [Re: Mycolorado]
    #28184358 - 02/12/23 04:42 PM (1 year, 10 months ago)

LOL my bad, I copy pasted text from my other post related to tiger dropping -_-


--------------------
#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold

Extras: Filter Print Post Top
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