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nakadash1
Stranger
Registered: 11/02/22
Posts: 156
Last seen: 1 year, 2 months
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How did I contaminate this plate so badly? (pics)
#28184308 - 02/12/23 04:12 PM (1 year, 10 months ago) |
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Both plates were inoculated from an agar-agar plate (deep poured, 3-4cm) with minimal surface growth. Both transfers were performed in a SAB. The only thing I can think of is perhaps condensation could have helped spread spores all over the plate?
Even the amount of growth is wildly different for some reason -- both were inoculated 5 days ago, the medium is exactly the same although it is about half the thickness on the larger plate.
*Edit reposted the wrong text -_-

-------------------- #1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold
Edited by nakadash1 (02/12/23 04:43 PM)
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stubb
Dahg Rastubfari


Registered: 03/23/19
Posts: 1,447
Loc: Memory
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Re: How did I clamtanibate this plot so boodly? (pics) [Re: nakadash1]
#28184327 - 02/12/23 04:24 PM (1 year, 10 months ago) |
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See Tic Tac Toe: https://www.shroomery.org/forums/showflat.php/Number/22601023
OP fixed his post, I swear I'm not just shouting random teks at passersby like the town drunk
Edited by stubb (02/12/23 05:46 PM)
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nakadash1
Stranger
Registered: 11/02/22
Posts: 156
Last seen: 1 year, 2 months
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Re: How did I contaminate this plate so badly? (pics) [Re: stubb]
#28184340 - 02/12/23 04:35 PM (1 year, 10 months ago) |
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Quote:
stubb said: See Tic Tac Toe: https://www.shroomery.org/forums/showflat.php/Number/22601023
I think you meant to reply to my other thread, thank you though! I think I'll try that also. I figure it's a tradeoff between more inoculation points vs contamination risk.
-------------------- #1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
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Re: How did I contaminate this plate so badly? (pics) [Re: nakadash1]
#28184351 - 02/12/23 04:38 PM (1 year, 10 months ago) |
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I’m confused.
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Land Trout
Stranger



Registered: 01/08/18
Posts: 3,180
Last seen: 7 hours, 4 minutes
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Re: How did I contaminate this plate so badly? (pics) [Re: Mycolorado]
#28184356 - 02/12/23 04:41 PM (1 year, 10 months ago) |
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Quote:
Mycolorado said: I’m confused. 
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nakadash1
Stranger
Registered: 11/02/22
Posts: 156
Last seen: 1 year, 2 months
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Re: How did I contaminate this plate so badly? (pics) [Re: Mycolorado]
#28184358 - 02/12/23 04:42 PM (1 year, 10 months ago) |
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LOL my bad, I copy pasted text from my other post related to tiger dropping -_-
-------------------- #1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold
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