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Offlineshmonger
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Registered: 08/18/21
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Funky agar?
    #28183912 - 02/12/23 11:48 AM (1 year, 10 months ago)

This is RustyWhite, 2nd transfer started from spore swab. This particular cup was started on 2/7. I think there's some pretty good looking myc here, but I'm concerned about the cloudy white filmy stuff you can see around the edges. I used the no-pour oven tek method to create these agar cups and a few showed contamination after a couple days. Is that what I'm seeing here? Is it worth going ahead with this cup or should I trash it? Thanks for your advice!



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Invisibledowodenum
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Registered: 08/05/19
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Re: Funky agar? [Re: shmonger] * 1
    #28183955 - 02/12/23 12:22 PM (1 year, 10 months ago)

That's a huuuge chunk of transfer, which:

- gives you less room to work
- less time to take a transfer from the leading edge without it hitting the sides of the plate
- more likely that you brought a contam from the donor plate

From what I've read, that spikey look (rhizomorphic growth) can be a sign that it's trying to outrun a contam, but I also think it can happen naturally when the myc sectors against itself...

I'd suggest taking smaller transfer samples, trying to get them dead-center in the plate, and transferring away when the myc is a comfortable distance from the source transfer. I've read that water agar can be great for this because since there's no nutrients, the myc has to grow a lot faster to explore, and that means you can jump on it sooner.

Check out D3's perfect transfer tek and get a punch like his - then you can easily take consistent sized transfers. I pick them up with a syringe needle on the end of a long nail.


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InvisibleLeftfield420
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Registered: 02/26/16
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Re: Funky agar? [Re: shmonger] * 1
    #28184147 - 02/12/23 02:16 PM (1 year, 10 months ago)

Quote:

shmonger said:
This is RustyWhite, 2nd transfer started from spore swab. This particular cup was started on 2/7. I think there's some pretty good looking myc here, but I'm concerned about the cloudy white filmy stuff you can see around the edges. I used the no-pour oven tek method to create these agar cups and a few showed contamination after a couple days. Is that what I'm seeing here? Is it worth going ahead with this cup or should I trash it? Thanks for your advice!








kinda looks like bacteria to me, does it look milky to you? if so that's bacteria for sure

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Offlineshmonger
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Registered: 08/18/21
Posts: 102
Last seen: 2 days, 2 hours
Re: Funky agar? [Re: Leftfield420]
    #28184255 - 02/12/23 03:43 PM (1 year, 10 months ago)

Yes, I think milky is a good description. :thanx:


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Offlineshmonger
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Last seen: 2 days, 2 hours
Re: Funky agar? [Re: dowodenum] * 1
    #28184257 - 02/12/23 03:44 PM (1 year, 10 months ago)

Thanks for the tips - I will work on improving my transfer technique! :thanx:


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InvisibleLeftfield420
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Registered: 02/26/16
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Re: Funky agar? [Re: shmonger] * 1
    #28184266 - 02/12/23 03:45 PM (1 year, 10 months ago)

you'll get it... practice makes perfect🀘

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