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Offlinephenyl
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28306192 - 05/04/23 10:18 AM (1 year, 8 months ago)

Quote:

MushyMom said:
Pairing these APE jars today and sending to mini monos - doing a 1:3 ratio with a pseudo casing layer.

Overall these jars seem to look ok. They show some condensation and signs of bacteria but it seems mild.  These were prepared using the flash soak method described above. The jars seemed a smidge too cooked and I reduced the soak time by a couple of minutes when I prepped the next batch in that method. 






So you still haven't figured out where the bacteria is coming from? Only thing I can add is that halving the volume of grain in the jars will reduce your grain expenditure and (somewhat) decrease time to colonization while you test things.


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: phenyl] * 1
    #28307075 - 05/04/23 11:00 PM (1 year, 8 months ago)

The PE shoeboxes made from the wbs produced lots and lots of blobs.



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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28307077 - 05/04/23 11:02 PM (1 year, 8 months ago)

Sent these today to mini monos - GT this round


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: phenyl] * 1
    #28307079 - 05/04/23 11:06 PM (1 year, 8 months ago)

Quote:

phenyl said:

So you still haven't figured out where the bacteria is coming from? Only thing I can add is that halving the volume of grain in the jars will reduce your grain expenditure and (somewhat) decrease time to colonization while you test things.




I think I’m closing in on it - pretty sure it’s now on the grain prep.  I need to find the sweet spot for the soak or the flash prep method.  It’s close - I can feel it LOL

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28312542 - 05/09/23 10:43 AM (1 year, 8 months ago)

Crossing fingers and toes - these are the APE boxes I sent on 5-3


Edited by MushyMom (05/09/23 11:42 AM)

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28321218 - 05/15/23 11:11 PM (1 year, 7 months ago)

The remaining APE box - yesterday vs today. Today we have the fuzzy spots. They were sent 12 days ago and I’m getting nervous.


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Offline3.A.M
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28321282 - 05/16/23 01:04 AM (1 year, 7 months ago)

Quote:

MushyMom said:
The remaining APE box - yesterday vs today. Today we have the fuzzy spots. They were sent 12 days ago and I’m getting nervous.





Errrr nerrrr, looks like mould :frown:


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: 3.A.M]
    #28322678 - 05/17/23 01:16 AM (1 year, 7 months ago)

Not sure wtf is happening here


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28322680 - 05/17/23 01:18 AM (1 year, 7 months ago)

Gt bin sent on 5-4


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28323474 - 05/17/23 03:02 PM (1 year, 7 months ago)

GT tubs sent 5-4


Edited by MushyMom (05/17/23 03:03 PM)

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28323753 - 05/17/23 06:30 PM (1 year, 7 months ago)

This one has been evicted from the house.  Oh well.



Moving back into shoeboxes with the next ones I’m sending.  Those are the jars that I soaked but I don’t think they rehydrated well enough and they colonized slower. But they are colonized so why not?

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OfflinePandaskis
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28324032 - 05/17/23 09:20 PM (1 year, 7 months ago)

Quote:

MushyMom said:
This one has been evicted from the house.  Oh well.



Moving back into shoeboxes with the next ones I’m sending.  Those are the jars that I soaked but I don’t think they rehydrated well enough and they colonized slower. But they are colonized so why not?




Oh man thats some weird growth. Shame because your surface conditions look spot on. im curious about what may be going on; how was your tub getting FAE?

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Offlinejohnukguy
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28324256 - 05/18/23 01:37 AM (1 year, 7 months ago)

I may have missed it, if you've done this already, but can you post a step by step up the point of S2B? I realise it's a lot to type out, but there's got to be a solution. This isn't some supernatural occurrence, so the contamination has a cause and that can be found and dealt with.


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: johnukguy] * 1
    #28325673 - 05/19/23 12:32 AM (1 year, 7 months ago)

Quote:

johnukguy said:
I may have missed it, if you've done this already, but can you post a step by step up the point of S2B? I realise it's a lot to type out, but there's got to be a solution. This isn't some supernatural occurrence, so the contamination has a cause and that can be found and dealt with.




Yeah I’ve been doing a lot of batches so they are running together.

So I had prepped 8 jars - I started by washing and adding the drop of dish soap. Rinsed it clear. Brought water to a boil. Turned off the heat and moved the pot and added the millet. I let it soak for 20 minutes then poured it out and rinsed and drained for about an hour. Loaded into jars and Pcd for about 2 hours - I put the exact amount up there originally and yes I vented first. Half of the jars has unmodified plastic lids with a full sfd disc under the lid and the other half were standard modified metal lids.

I used LC that I had purchased from a shroomery supplier that I had tested. I’m concurrently working on my agar technique but that takes so much time and I’m wanting to work on my grain before the summer season kicks in.

I had also done a second batch of millet jars that I had soaked for 15 hours (after washing with dish soap) and then loaded into jars (same lids as described above) and pcd. Again I’m not sure the exact time - but I had posted above.

So in the end - I used APE lc with half (8) of the jars and GT lc with the other half of the jars. I split them up by the method of preparation so 4 APE jars with the flash boil soak method and 4 with the overnight soak method. Then I split the GT the same way - if that makes sense.

The grain that was just soaked wasn’t as hydrated. I considered tossing them but that millet is expensive at the wild bird store - and took longer to colonize. I’m about to send those jars but the other jars colonized faster.

I used canna coir blocks - I mixed 650 g of coir with 3 1/2-4 quarts of boiling water and 2 cups of verm. I have a dedicated coir bucket that I wash out with anti bacterial soap and hot water and then dry completely before use.

I sent them to mini monos - the 20 quart gasket tubs. I had concerns that contamination could possibly be in the actual gaskets - but I’m not sure how to address that. I washed them with antibacterial soap and hot water but I still don’t trust them. These mini monos have the holes drilled around the sides for air exchange - like the pasty ez dial tubs.

I mixed the spawn at a 3:1 ratio with the substrate and then added another 2 quarts on top for a pseudo casing layer. I also compressed as much as I could

The 2 APE bins seemed to fall - one went to trich pretty fast - the other is now outside. Not sure what is happening but it can do what it wants outside I guess.

The two GT bins from this batch are starting to colonize. I’m just crossing fingers and toes that they make it.

My suspicions are as follows - it could be any or all of the following
1. The LC wasn’t good
2. The grain wasn’t properly prepped (I have a tfal that is supposed to go to 15 psi) instead of a presto)
3. Contamination was present in the jars before I sent to fruiting
4 contamination was present in the bins in the gaskets.
5 contamination was present in the air since I’ve had a few bins go bad

If the GT tubs turn out ok then I can rule out the grain prep.

I will need to send the rest of the jars (the soaked ones) and see how they goes. I was planning to send them all to shoeboxes just in case. If the APE goes bad then I will look at the LC from the vendor. If it doesn’t then I will be looking at the gasket tubs.  It’s also possible they will all go bad because I don’t know if the grain was fully hydrated. But we will cross that bridge when we get there.

Hope that all makes sense

Does that make sense?  It’s late.

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28329195 - 05/21/23 08:04 PM (1 year, 7 months ago)

I think these are fucked


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OfflinePandaskis
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28329264 - 05/21/23 09:56 PM (1 year, 7 months ago)

Thats super weird growth... It still looks like mycelium... But man it kinda looks fucked

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OfflinePBJ710
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Re: Diagnosing my contamination issues/reviewing technique [Re: Pandaskis] * 1
    #28329371 - 05/21/23 11:27 PM (1 year, 7 months ago)

I believe Canna has historically added trichoderma to their coco to aid in plant health - it might be worth trying another brand of coco to see if that changes things for you.  It seems like you're addressing pretty much everything else already. :justdontknow:

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Offlinejohnukguy
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Re: Diagnosing my contamination issues/reviewing technique [Re: PBJ710]
    #28329383 - 05/22/23 12:08 AM (1 year, 7 months ago)

:whathesaid: Is definitely worth checking out. And thank you for all that you typed up. It does help.

At this point, I would suggest three things - to try different grain, even something like plain brown rice, or cheap wild bird seed, whatever you can easily get that's not expensive for a small batch. Plain brown rice for example is pretty clean out of the bag. It tends to get starchy but that's not a major issue I think.

Secondly, different coir. I really do, in my thinking on this, keep coming back to that coir. You could even do a tub, as you normally would, but not spawn to it and see if that contaminates with a few weeks. Four at most should give you an answer on that.

I'd also recommend adding another fifteen minutes or so to the PC time, just in case it's not actually reaching fifteen psi. Having said this, even if it's thirteen or fourteen psi, two hours should still be enough. But, just in case.

The 'proper' way to do such things is to change one variable at a time. If it was me, I'd do all three, because I'm impatient with such things, but that's me and not necessarily recommended here.

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: PBJ710] * 1
    #28329431 - 05/22/23 01:40 AM (1 year, 7 months ago)

Quote:

PBJ710 said:
I believe Canna has historically added trichoderma to their coco to aid in plant health - it might be worth trying another brand of coco to see if that changes things for you.  It seems like you're addressing pretty much everything else already. :justdontknow:




This was my first time using canna - I’ve used coco bliss in the past and also had it go bad. I checked the site for canna and it says that they don’t add trich but they also said that basically trich happens - it’s everywhere. 

I know that I recently super baccy jars to shoeboxes and didn’t have any go green. This was the last batch I had posted before these. I feel like I need a better journal to keep track of all of this lol. They weren’t great but I still got a couple oz of awesome blobs.  Besides being shoeboxes - I also used a lower ratio or substrate to spawn to compensate for the bacterial spawn. 

Next steps - I will be sending what I have to shoeboxes next.  I’m thinking about also lowering the substrate to spawn ratio to mimic what I had done with the bacterial spawn.

I still have a decent amount of millet and I prefer it because I feel like I can see what’s happening better because it’s more uniform. It’s a little frustrating because I feel like the grain prep has been getting a lot better but I’m still getting crap. I have pics of the jars I sent posted above - I feel like they were solid - but I could be wrong.

If anyone has any idea on how to better journal this - I would love to know.

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28343004 - 06/01/23 11:54 AM (1 year, 7 months ago)



Tested additional jars with canna coir in shoeboxes. Created 4 on 5-24. So far 2 have contaminated. Next test will be to test additional jars (already ready) with coco bliss and no vermiculite in shoeboxes.

Other considerations - I still have concerns that my tfal pressure cooker is not reaching a full 15 psi. Will pc next batch longer to see if same results are achieved. If so, will be purchasing a presto.

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