|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: johnukguy]
#28285703 - 04/19/23 04:32 PM (1 year, 8 months ago) |
|
|
Quote:
johnukguy said: I could be wrong on this, especially since I usually use honey instead of LME, but I don't think that you need both LME and Karo.
Those are 3 different recipes - Someone mentioned that telephone agar can sometimes be harder…. So I’m trying telephone agar with LME and another with telephone agar with potato flakes and Karo. And then I have another brand of agar I’m trying. I had it all in my cult cupboard so I figured why not
|
johnukguy
Learning



Registered: 06/23/22
Posts: 2,423
Loc: Colorado
Last seen: 1 year, 2 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28285711 - 04/19/23 04:36 PM (1 year, 8 months ago) |
|
|
Ah got ya. That makes sense then.
-------------------- How to post pictures to shroomery TEK
Shroomery Trusted Cultivator And Member YouTube Channels.
“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: Pandaskis] 1
#28285713 - 04/19/23 04:37 PM (1 year, 8 months ago) |
|
|
Quote:
Pandaskis said:
Quote:
MushyMom said: All my grains seem to have stalled. All my plates seem to have stalled. Is my closet too cold? Not sure. Just kind of frustrated
Whats your ambient temperature in your closet?
Any images of your grain/plates?
How long have they stalled for in your estimate?
The closet is about 70 degrees - last winter was warmer and it was closer to 72 in there.
All my plates have been sucking lately - I tried from spores, from LC and even a clone and they all got really sad growth. So I am making 3 new batches to see if it’s the agar or if it’s the technique. These are the plates that are currently in there. That’s the last of that batch of agar plates - I had better plates from the batch before. Like the plates from spores all started pinning. These just look sad

These are the jars - I have pictures posted above of this batch - it was from agar to WBS - originally sent on 3-20. I’m not seeing a lot of progress in these jars finishing up. Considering turning the heat up a degree or two but they all look bacterial as fuck so part of me doesn’t want to bother. Those are the last batch with unmodified lids.

These are the newer batches with millet - one was prepared through the fooman method where you boil water - remove from heat and add millet. Those jars are looking better. The other batch was made by soaking millet overnight for 15 hours. I shook these 2 days ago and the soaked batch doesn’t seem to be recovering

I got a lot of stuff going on here…. LOL
Edited by MushyMom (04/19/23 04:38 PM)
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: Way]
#28285736 - 04/19/23 04:51 PM (1 year, 8 months ago) |
|
|
Quote:
Way said:
Quote:
MushyMom said: 2 weeks in - and I’m giving up. They are turning orange. It’s all good. This was my first batch - the control group.

Did you end up throwing these away? I wouldn't have given up hope for at least some fruits personally.
They were definitely too wet and bacterial. More airflow would have helped. Not sure what your substrate recipe was but it could have been too wet as well.
I just checked them - here’s what I found
|
Pandaskis
Eating Bamboo



Registered: 03/14/23
Posts: 1,869
Last seen: 8 months, 8 days
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] 1
#28285741 - 04/19/23 04:57 PM (1 year, 8 months ago) |
|
|
Hmmmmm, im perplexed about what could be going wrong, your technique seems pretty good from what im reading, have you tried doing BRF puck to Agar? I heard people have more luck with germinating spores especially if its in a syringe that way.
Do you happen to use a swamp cooler?
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28286268 - 04/20/23 01:01 AM (1 year, 8 months ago) |
|
|
Quote:
MushyMom said: Making a note for myself - 3 bottles of agar
1. 7 g telegram 8 g LME 400 ml water. No color. 2. 8 g Hoosier Hill Agar 8 g LME 400 ml water- red 3. 6g Karo 2 g potato flakes 7 g telephone agar 400 ml water - green Tried doing more potato flakes but couldn’t get them to dissolve
My arm is going to be SO SORE tonight
Weird stuff. I couldn’t pour right away - so I left the bottles in the instant pot. I came back and when I opened it -
1 had somehow overflowed and only about 200 ml left in the bottle - the rest was in the instant pot. It’s one of the blue lid bottles - I’m only going to use orange lids from now on
I turned the heat back on and bottle 2 is melting and bubbling like crazy. Meanwhile bottles 1 and 3 are slowly starting to melt. Bottle 2 was made with the Hoosier Hill agar - I had bought a pound for $30 when I first got started. I was hoping to compare side by side with bottle 1 but there may not be enough agar left to pour - what’s in the bottle is super hard.
Waiting for them to melt and then I can get some sleep. It’s been a busy day
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28286676 - 04/20/23 11:14 AM (1 year, 8 months ago) |
|
|
This one is fucked

The jars prepared with the flash soak method are recovering much better than the jars with the 15 hour soak. It may have been too cold in the house during the soak - they didn’t rehydrate as well

And these - I’m straight up giving up on. I am leaning towards the modified lids being the issue but need to do a batch with millet and agar to be sure

I have baccy blobs in my bins and I’m happy about that
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] 1
#28286682 - 04/20/23 11:20 AM (1 year, 8 months ago) |
|
|
Quote:
MushyMom said:
Quote:
MushyMom said: Making a note for myself - 3 bottles of agar
1. 7 g telegram 8 g LME 400 ml water. No color. 2. 8 g Hoosier Hill Agar 8 g LME 400 ml water- red 3. 6g Karo 2 g potato flakes 7 g telephone agar 400 ml water - green Tried doing more potato flakes but couldn’t get them to dissolve
My arm is going to be SO SORE tonight
Weird stuff. I couldn’t pour right away - so I left the bottles in the instant pot. I came back and when I opened it -
1 had somehow overflowed and only about 200 ml left in the bottle - the rest was in the instant pot. It’s one of the blue lid bottles - I’m only going to use orange lids from now on
I turned the heat back on and bottle 2 is melting and bubbling like crazy. Meanwhile bottles 1 and 3 are slowly starting to melt. Bottle 2 was made with the Hoosier Hill agar - I had bought a pound for $30 when I first got started. I was hoping to compare side by side with bottle 1 but there may not be enough agar left to pour - what’s in the bottle is super hard.
Waiting for them to melt and then I can get some sleep. It’s been a busy day
I was only able to pour dishes from the Hoosier hill agar and mle. The potato never quite dissolved right.
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] 1
#28288269 - 04/21/23 01:43 PM (1 year, 8 months ago) |
|
|
I think I’m finding a sweet spot - millet - flash soaked for 20 minutes - and more FAE in the jars. Both the plastic lid with the SFD disk and the modified lid seem to be doing well.
|
Pandaskis
Eating Bamboo



Registered: 03/14/23
Posts: 1,869
Last seen: 8 months, 8 days
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28288272 - 04/21/23 01:45 PM (1 year, 8 months ago) |
|
|
Quote:
MushyMom said: I think I’m finding a sweet spot - millet - flash soaked for 20 minutes - and more FAE in the jars. Both the plastic lid with the SFD disk and the modified lid seem to be doing well.

Theyre looking good to me!
|
Way
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28288275 - 04/21/23 01:46 PM (1 year, 8 months ago) |
|
|
Those definitely look pretty promising so far.
--------------------
That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
|
johnukguy
Learning



Registered: 06/23/22
Posts: 2,423
Loc: Colorado
Last seen: 1 year, 2 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28288901 - 04/21/23 07:00 PM (1 year, 8 months ago) |
|
|
Quote:
MushyMom said: I think I’m finding a sweet spot - millet - flash soaked for 20 minutes - and more FAE in the jars. Both the plastic lid with the SFD disk and the modified lid seem to be doing well.

Most agreeable.
-------------------- How to post pictures to shroomery TEK
Shroomery Trusted Cultivator And Member YouTube Channels.
“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28290081 - 04/22/23 03:44 PM (1 year, 8 months ago) |
|
|
Ugh. Bacterial
|
johnukguy
Learning



Registered: 06/23/22
Posts: 2,423
Loc: Colorado
Last seen: 1 year, 2 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28290107 - 04/22/23 03:56 PM (1 year, 8 months ago) |
|
|
If it's just the one jar that's bacterial, then I think you're doing pretty well.
-------------------- How to post pictures to shroomery TEK
Shroomery Trusted Cultivator And Member YouTube Channels.
“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
|
phenyl
Stupid person



Registered: 09/03/22
Posts: 544
Loc: One toke over the line
Last seen: 7 hours, 41 minutes
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] 1
#28290179 - 04/22/23 04:25 PM (1 year, 8 months ago) |
|
|
Quote:
MushyMom said: Ugh. Bacterial
Story of my life. Bacterial aerosols getting everywhere in the SAB environment and not settling presumably killed my grow for about 6 months last year. Keep at it.
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: johnukguy] 1
#28290523 - 04/22/23 08:08 PM (1 year, 8 months ago) |
|
|
Quote:
johnukguy said: If it's just the one jar that's bacterial, then I think you're doing pretty well.
One also went to mold 
I think the millet I did the fooman method on - added to boiling hot water for 20 minutes are a smidge too wet. And the millet I did the 15 hour soak on are a smidge too dry. But there’s a happy spot right in between and I’m almost there
|
johnukguy
Learning



Registered: 06/23/22
Posts: 2,423
Loc: Colorado
Last seen: 1 year, 2 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
#28290681 - 04/22/23 11:28 PM (1 year, 8 months ago) |
|
|
Not sure if you've seen this video, from Stipe-n Cap, which I believe is based on Fooman's prep, but it's working well for me so far, in case there is a difference with what you're doing:
Also, I don't know how you're consuming the fruits, but I do find a difference if I make a tea, or use capsules instead. The capsules come on slower and more gently but seem a little less intense overall. Then again, I'm testing the capsules for people that have conditions that can benefit from the mushrooms, but don't necessarily want to trip and find it useful to have a more precise way of measuring amounts. And that likely means that I have some tolerance as it's only been a week between doing that and consuming a larger amount.
-------------------- How to post pictures to shroomery TEK
Shroomery Trusted Cultivator And Member YouTube Channels.
“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
|
NFLProof
Sponge



Registered: 05/31/18
Posts: 922
Loc: In the ether
Last seen: 25 days, 16 hours
|
Re: Diagnosing my contamination issues/reviewing technique [Re: johnukguy] 1
#28290733 - 04/23/23 12:44 AM (1 year, 8 months ago) |
|
|
I just did this now....last 5 minutes in the PC then off to bed.
-------------------- Yeah, yeah.
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: johnukguy] 1
#28290734 - 04/23/23 12:44 AM (1 year, 8 months ago) |
|
|
Quote:
johnukguy said: Not sure if you've seen this video, from Stipe-n Cap, which I believe is based on Fooman's prep, but it's working well for me so far, in case there is a difference with what you're doing:
Yeah I was going off his millet prep - the fooman prep where you being the water to a boil - remove from heat and let the grain soak - I think it might have soaked a smidge too long. Vs doing a 15 hour soak - I think the water needed to be a little warmer for the jars I soaked overnight. But I feel like I’m close. I like the millet more than the wbs because it’s more consistent
|
MushyMom
Stranger
Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
|
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] 2
#28305159 - 05/03/23 04:04 PM (1 year, 8 months ago) |
|
|
Pairing these APE jars today and sending to mini monos - doing a 1:3 ratio with a pseudo casing layer.
Overall these jars seem to look ok. They show some condensation and signs of bacteria but it seems mild. These were prepared using the flash soak method described above. The jars seemed a smidge too cooked and I reduced the soak time by a couple of minutes when I prepped the next batch in that method.
|
|