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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28275272 - 04/13/23 01:19 AM (1 year, 9 months ago)

I’m really not encouraged with the state of the ones I sent to fruiting. 

I flipped lids on the shoeboxes but I’m concerned that they aren’t pinning and seem to have a fluffy film on them. The second one is in a bag and even though I have the top of the bags open - they are still wet


Edited by MushyMom (04/13/23 01:21 AM)

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Offlinejohnukguy
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28275275 - 04/13/23 01:22 AM (1 year, 9 months ago)

The water droplets on the substrate surface look a little on the large side to me but my eyesight isn't that good, so I'd wait on a few more opinions on that. How many days since you spawned to bulk? And I'm assuming that the shoeboxes are getting air and some light from the get go?


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28276123 - 04/13/23 04:16 PM (1 year, 9 months ago)

Going back to batch 2 - WBS and unmodified lids - using the agar plates I posted before.  I dropped a wedge or two into each of these.  These were done on 3-20 and shaken a week or so ago - and I have many concerns.  I’m wondering if the uneven colonization is from using wedges instead of LC or smaller chunks of agar.
These are the last of the jars with unmodified lids and no filter.  Next couple batches are made with millet and SFDs or modified lids.

Jar 1


Jar 2 - more moisture :frown:



Jar 3 - one side is colonized - the other isn’t.




Jar 4


Jar 5


Jar 6

Edited by MushyMom (04/13/23 04:21 PM)

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Offlinejohnukguy
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28276334 - 04/13/23 06:36 PM (1 year, 9 months ago)

I've only done agar to grain, with brown rice and bird seed, and those jars look fine to me at the moment. My jars also colonize somewhat unevenly. I think that so long as the colonization isn't taking unduly long, or there aren't areas that just won't colonize, then you should be good.


--------------------
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“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: johnukguy] * 1
    #28277080 - 04/14/23 09:15 AM (1 year, 8 months ago)

Quote:

johnukguy said:
I've only done agar to grain, with brown rice and bird seed, and those jars look fine to me at the moment. My jars also colonize somewhat unevenly. I think that so long as the colonization isn't taking unduly long, or there aren't areas that just won't colonize, then you should be good.



I usually go off Stipes checklist for clean spawn and they all pretty much fail LOL! I’m really concerned about the condensation

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OfflinePandaskis
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28277220 - 04/14/23 10:44 AM (1 year, 8 months ago)

Quote:

MushyMom said:
Quote:

johnukguy said:
I've only done agar to grain, with brown rice and bird seed, and those jars look fine to me at the moment. My jars also colonize somewhat unevenly. I think that so long as the colonization isn't taking unduly long, or there aren't areas that just won't colonize, then you should be good.



I usually go off Stipes checklist for clean spawn and they all pretty much fail LOL! I’m really concerned about the condensation




Condensation is fine, unless it starts pooling, there is quite a bit of moisture in some of your jars, but i dont think its quite enough to say its bacterial. WBS also has a tendency to look a little bit bacterial
even if its not  especially if you have cracked corn in it, because the starchy and stickiness can leave what looks like biofilm or wet spots. Just let it play out, they look fine to me atm :smile:

It looks pretty similar to my grain spawn, i appreciate the time and detail you put into this, helps noobies like me!

WBS 2nd attempt

Edited by Pandaskis (04/14/23 11:04 AM)

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28277248 - 04/14/23 11:06 AM (1 year, 8 months ago)

Not optimistic about these - it’s been 11 days

Shoeboxes


Bags - these have been completely open for air flow but still really wet

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OfflinePandaskis
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28277261 - 04/14/23 11:11 AM (1 year, 8 months ago)

Quote:

MushyMom said:
Not optimistic about these - it’s been 11 days

Shoeboxes


Bags - these have been completely open for air flow but still really wet





The mycelium in the shoebox looks a little matted up to my eyes, i wonder if you may be over misted?  Not sure!

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: Pandaskis]
    #28277266 - 04/14/23 11:12 AM (1 year, 8 months ago)

Quote:

Pandaskis said:
Quote:

MushyMom said:
Not optimistic about these - it’s been 11 days

Shoeboxes


Bags - these have been completely open for air flow but still really wet





The mycelium in the shoebox looks a little matted up to my eyes, i wonder if you may be over misted?  Not sure!



I haven’t misted at all…. That’s part of what concerns me…

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OfflinePandaskis
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28277275 - 04/14/23 11:17 AM (1 year, 8 months ago)

Quote:

MushyMom said:
Quote:

Pandaskis said:
Quote:

MushyMom said:
Not optimistic about these - it’s been 11 days

Shoeboxes


Bags - these have been completely open for air flow but still really wet





The mycelium in the shoebox looks a little matted up to my eyes, i wonder if you may be over misted?  Not sure!



I haven’t misted at all…. That’s part of what concerns me…





Hmmm, how was the field capacity on the coir for those shoeboxes?  I wonder if you want to cut the liner more flush with the substrate so that the water from the condensation doesn't drip down onto the mycelium and pool. The mycelium around the edges just look a bit strange to me. But wait for someone with a bit more experience to chip in :laugh:

Edited by Pandaskis (04/14/23 11:20 AM)

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Offlinejohnukguy
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28277417 - 04/14/23 01:10 PM (1 year, 8 months ago)

I'm inclined to think that giving a little more air would help.


--------------------
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“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28280588 - 04/16/23 01:35 PM (1 year, 8 months ago)

2 weeks in - and I’m giving up. They are turning orange. It’s all good. This was my first batch - the control group.


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28283839 - 04/18/23 01:55 PM (1 year, 8 months ago)

All my grains seem to have stalled.  All my plates seem to have stalled.  Is my closet too cold?  Not sure.  Just kind of frustrated

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OfflineThrowaway
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28283841 - 04/18/23 01:57 PM (1 year, 8 months ago)

Quote:

MushyMom said:
Is my closet too cold?



Maybe tell us the temp?

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OfflinePandaskis
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28283842 - 04/18/23 01:58 PM (1 year, 8 months ago)

Quote:

MushyMom said:
All my grains seem to have stalled.  All my plates seem to have stalled.  Is my closet too cold?  Not sure.  Just kind of frustrated




Whats your ambient temperature in your closet?

Any images of your grain/plates?

How long have they stalled for in your estimate?

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InvisibleWay
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28283847 - 04/18/23 02:02 PM (1 year, 8 months ago)

Quote:

MushyMom said:
2 weeks in - and I’m giving up. They are turning orange. It’s all good. This was my first batch - the control group.






Did you end up throwing these away? I wouldn't have given up hope for at least some fruits personally. 

They were definitely too wet and bacterial. More airflow would have helped. Not sure what your substrate recipe was but it could have been too wet as well.


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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: Way]
    #28285632 - 04/19/23 03:35 PM (1 year, 8 months ago)

Quote:

Way said:
Quote:

MushyMom said:
2 weeks in - and I’m giving up. They are turning orange. It’s all good. This was my first batch - the control group.






Did you end up throwing these away? I wouldn't have given up hope for at least some fruits personally. 

They were definitely too wet and bacterial. More airflow would have helped. Not sure what your substrate recipe was but it could have been too wet as well.



They aren’t thrown away yet because I’m lazy but I’m thinking the issue is 2 fold - the substrate was probably too wet and the jars were questionable to begin with.

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OfflineMushyMom
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28285678 - 04/19/23 04:15 PM (1 year, 8 months ago)

Making a note for myself - 3 bottles of agar

1. 7 g telegram 8 g LME 400 ml water.  No color.
2. 8 g Hoosier Hill Agar 8 g LME 400 ml water- red
3. 6g Karo 2 g potato flakes 7 g telephone agar 400 ml water - green Tried doing more potato flakes but couldn’t get them to dissolve

My arm is going to be SO SORE tonight

Edited by MushyMom (04/19/23 04:29 PM)

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Offlinejohnukguy
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28285681 - 04/19/23 04:17 PM (1 year, 8 months ago)

I could be wrong on this, especially since I usually use honey instead of LME, but I don't think that you need both LME and Karo.


--------------------
How to post pictures to shroomery TEK

Shroomery Trusted Cultivator And Member YouTube Channels.

“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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Invisiblemyc_ousin_vinny
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Re: Diagnosing my contamination issues/reviewing technique [Re: johnukguy]
    #28285684 - 04/19/23 04:19 PM (1 year, 8 months ago)

Quote:

johnukguy said:
I could be wrong on this, especially since I usually use honey instead of LME, but I don't think that you need both LME and Karo.




I think those are 3 diff recipes.


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