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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,852
Re: Recognizing and dealing with contamination [Re: AyePlus]
    #28176373 - 02/07/23 02:49 PM (1 year, 1 month ago)

Quote:

AyePlus said:
I’m also familiar with the same phenomenon as ladysknight. Way back in the day bod told me it was just bad bacteria but I wasnt convinced and also had a plate in cold storage grow out a funny halo




I’m under the impression its a mold that parasitizes mushrooms and sporulates on them, so a fruit swabbed or printed from an affected tub would have it too



Quote:

LadysKnight said:
Quote:

Nonagon said:
Quote:

Nonagon said:
Some follow up pics from my weird enmeshed mold plates. Rolled the dice and lost on this one :lol: Interesting seeing the mycelium go all crazy where it meets the contam though


Only leaves the footprint of the myc growth once it goes to grain, any further thoughts as to why this is?






Agar pics for fluffy/sketchy plate reference






I have a couple prints contaminated with this, I've tried for a year off and on to get away from it. I call it a parasitic white mold. It causes super slow growth at all stages and mostly aborts in a tub. Even plate pins and clone tissue has it. For me, I can see it as a clear, shiny halo that can be seen at the margins of germs and transfers with a bright light at just the right angle. Once the plate grows out it's invisible. This is why I can't fathom using any agar dish that's not perfectly clear.






I think this is some different kind of parasite similarly spread, which would explain a clean clone. Run that and print it and give up on this print IMO,

It might be a trichoderma species considering the enmeshed nsture, late appearance and green but hard to say. Would be interested to see it under a scope



Edit: just had the thought that since its running faster than the cube once it gets to a more nutrituios media (grain) maybe taking it to a lower or even no nutrient media like water agar would give the cube myc a chance to outpace it.




Yo bro, I'm glad you chimed in on this one, I've been dealing with this for years and was beginning to feel like the lone ranger. Doubting myself and everything I've been seeing. A lot of well respected cults and TCs seem unfamiliar and told me it was normal. Now I'm a new man. :smile:

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InvisibleLadyBird123S


Registered: 09/27/22
Posts: 263
Re: Recognizing and dealing with contamination [Re: LadysKnight]
    #28176391 - 02/07/23 03:01 PM (1 year, 1 month ago)

Cube agar to grain, fully colonized grain to pasteurized coir.
Is there any obvious cause for concern here?
It is not growing especially fast and the substrate want level, possibly accounting for the uncolonized coir.





Now a couple days later, had started giving more fae and what looks like some grey whispy’s are surfacing the uncolonized coir. Just hoping for some advice on whether I should wait it out, or if there’s something obvious happening that I don’t know how to see yet, and tossing would be best.


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OfflineAgent_Dale_Cooper
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Registered: 02/02/23
Posts: 13
Loc: Twin Peaks, WA, United States
Last seen: 1 year, 23 days
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28178240 - 02/08/23 08:01 PM (1 year, 1 month ago)

I have returned! And it doesn’t look like contamination to me! A little worried about that slightly off colored patch but *shrugs* looks like healthy mycelium to me

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OfflineSirPsycho
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Re: Recognizing and dealing with contamination [Re: Agent_Dale_Cooper] * 1
    #28178251 - 02/08/23 08:07 PM (1 year, 1 month ago)

Well, if it looks like it to you that's all that matters bubby. :kiss:


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OfflineMonkeyJesusFresco
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Re: Recognizing and dealing with contamination [Re: SirPsycho] * 2
    #28178314 - 02/08/23 08:25 PM (1 year, 1 month ago)

:whathesaid:

believe in urself

ethical responsibility edit:

in the interest of full disclosure, it's contamed as shit


--------------------
LAGM v 2.024
- endo cabendo

Edited by MonkeyJesusFresco (02/08/23 08:37 PM)

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Offlinerumfor69
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Re: Recognizing and dealing with contamination [Re: MonkeyJesusFresco] * 2
    #28178330 - 02/08/23 08:31 PM (1 year, 1 month ago)

Wait we're not telling them it looks like ice cream cause of bacteria and the yellowing is most likely from the same thing?


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OfflineMonkeyJesusFresco
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Re: Recognizing and dealing with contamination [Re: rumfor69] * 1
    #28178341 - 02/08/23 08:36 PM (1 year, 1 month ago)

done been told once already
and the bag owner said it looks good
sooooo...  yeah, send it and see what happens :shrug:


--------------------
LAGM v 2.024
- endo cabendo

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Offlinerumfor69
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Re: Recognizing and dealing with contamination [Re: MonkeyJesusFresco] * 1
    #28178357 - 02/08/23 08:46 PM (1 year, 1 month ago)

It may still throw some if fruited in the bag


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OfflineAgent_Dale_Cooper
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Registered: 02/02/23
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Re: Recognizing and dealing with contamination [Re: MonkeyJesusFresco]
    #28178385 - 02/08/23 09:03 PM (1 year, 1 month ago)

Wait, what signs do you see of contamination? Are you saying that because of the manure in the first photo I showed?

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OfflineSirPsycho
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Re: Recognizing and dealing with contamination [Re: Agent_Dale_Cooper] * 1
    #28178388 - 02/08/23 09:04 PM (1 year, 1 month ago)

Quote:

Agent_Dale_Cooper said:
Wait, what signs do you see of contamination? Are you saying that because of the manure in the first photo I showed?




Quote:

rumfor69 said:
Wait we're not telling them it looks like ice cream cause of bacteria and the yellowing is most likely from the same thing?




--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineStainless2
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Re: Recognizing and dealing with contamination [Re: rumfor69]
    #28178407 - 02/08/23 09:16 PM (1 year, 1 month ago)

From someone open to learning, thanks for that description (ice cream...lol)!  I have to say, y'all did your best to try to share your experience and knowledge, so thanks for that as well.

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Offlinenakadash1
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Re: Recognizing and dealing with contamination [Re: Stainless2]
    #28179693 - 02/09/23 03:34 PM (1 year, 1 month ago)

Does anybody know whether cubensis mycelium is *always* pure white, or are other colors possible on plates or in LC?


--------------------
#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold

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OfflineSirPsycho
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Re: Recognizing and dealing with contamination [Re: nakadash1]
    #28179749 - 02/09/23 04:16 PM (1 year, 1 month ago)

Quote:

nakadash1 said:
Does anybody know whether cubensis mycelium is *always* pure white, or are other colors possible on plates or in LC?



Well, there's white and ummm oh right, white.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineMonkeyJesusFresco
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Re: Recognizing and dealing with contamination [Re: nakadash1] * 1
    #28179811 - 02/09/23 04:53 PM (1 year, 1 month ago)

Quote:

nakadash1 said:
Does anybody know whether cubensis mycelium is *always* pure white, or are other colors possible on plates or in LC?




on plates you'll have different types of growth that may, at first, have you questioning wether or not it's a normal white or not, I can't speak for LCs 🤷🏻♂️ i don't do those enough.

on grains or cakes  it gets tricky cause you're having to make the judgement call through glass or plastic with the different colors of the grains mixed in there, but again, with experience (even just lurking on here and checking the pics people posts) you'll be able learn/twll the difference real quick

also, on plates you might see some discoloration of the agar itself if the myc digests the food coloring


--------------------
LAGM v 2.024
- endo cabendo

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Offlinerumfor69
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Re: Recognizing and dealing with contamination [Re: SirPsycho] * 1
    #28179890 - 02/09/23 05:50 PM (1 year, 1 month ago)

Quote:

SirPsycho said:
Quote:

nakadash1 said:
Does anybody know whether cubensis mycelium is *always* pure white, or are other colors possible on plates or in LC?



Well, there's white and ummm oh right, white.




Ooo Ooo SirP, do you remember that one time the cube myce was also white though!?


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OfflineNoobShroob
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Re: Recognizing and dealing with contamination [Re: rumfor69]
    #28180000 - 02/09/23 06:50 PM (1 year, 1 month ago)



sorry its weird but the second picture if from a few hours earlier. On the second one (which looks a lot healthier imo has a little black dot but hasnt seemed to have spread or changed size or anything. The jar on the left has a lot more moisture and what appears to be mycelium trying to run. Are they both good or whats the thoughts. im thinking if the right one looks good to yall it is also about ready for spawn.


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Offlinerumfor69
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Re: Recognizing and dealing with contamination [Re: NoobShroob]
    #28180166 - 02/09/23 08:52 PM (1 year, 1 month ago)

Looks like your grains were a little too wet when loaded into the jars. In the future take the extra time to wait for their surfaces to be completely dry.

The little black dot is another type of seed often found mixed in with oats, it's fine.

Otherwise I think they look ok so far. Probably wouldn't hurt to give them a shake and mix em up good and see if they bounce back quickly and reach 100% in a few days afterwards.


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OfflineGenome794
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Registered: 07/20/22
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Re: Recognizing and dealing with contamination [Re: rumfor69]
    #28180284 - 02/09/23 11:32 PM (1 year, 1 month ago)

1.
Nice looking plate - if your experience level is where I am at... But am I just isolating a pretty white mold. (in case it is good, I transfered anyway). Your opinion?Feb-7

Feb-9



2.
My effort on a hot pour may have paid off. Your opinion?


Edit: I took a few transfers from the colony at 2 o'clock - so it looks a bit messed up. IRL that area looks better than the growth in pic 1.

Edited by Genome794 (02/10/23 01:42 AM)

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Offlinerumfor69
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Re: Recognizing and dealing with contamination [Re: Genome794]
    #28180318 - 02/10/23 12:57 AM (1 year, 1 month ago)

1. Hmmm it does look very disorganized like mold. Developing water droplets on it looks sus too maybe. I'm not real sure but it doesn't look right to me. Let's see what others think.

2. Looks like multiple kinds of mold to me I don't think there's anything there you want.


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OfflineGenome794
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Re: Recognizing and dealing with contamination [Re: rumfor69]
    #28180336 - 02/10/23 01:45 AM (1 year, 1 month ago)

Quote:

rumfor69 said:

2. Looks like multiple kinds of mold to me I don't think there's anything there you want.




Thanks, that plate was/is bad. It looks like that after an agar hot pour but I am out of options...

I edited the other post - I took transfers from the colony at 2 o'clock, so it doesnt look to uniform anymore.

I have another set of the same culture that has gone through a peroxide wash - grabbing at straws to clean the culture up enough to start transfering away.

Edited by Genome794 (02/10/23 01:51 AM)

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