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OfflineBlueAndOrange
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Re: Psilocybe natalensis [Re: Eugene Gesuale]
    #28174953 - 02/06/23 02:07 PM (1 year, 9 days ago)

Quote:

Adrenognome said:
Are you also able to test for the other active compounds?




No. I’m using off the shelf testing. I don’t have access to a full lab.


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OfflineMushymang
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Re: Psilocybe natalensis [Re: BlueAndOrange]
    #28175203 - 02/06/23 06:08 PM (1 year, 9 days ago)

Very nice experiment


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OfflineMarcus666
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Re: Psilocybe natalensis [Re: Mushymang] * 4
    #28175820 - 02/07/23 07:10 AM (1 year, 9 days ago)

Here we go...



Half a dozen plates with signs of life, all look clean so far too.

:aliendance:

:psychsplit:


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InvisibleSeeker604
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Re: Psilocybe natalensis [Re: Marcus666]
    #28176092 - 02/07/23 11:08 AM (1 year, 8 days ago)

Hello friends,

I’ve spent the last two days’ free time reading this thread from the beginning, and the history is fascinating. I’m receiving two varieties of Ps. Natalensis this week from Yoshi, and I wanted to confirm what the current parameters are for successful spawn and fruiting runs.

I’m into the 100s of the thread, and up to that point the convention is to use tomentose mycelium to avoid overlay issues, and to use a substrate based on hpoo/verm/coir, and to keep the spawn/sub ratio low like 1:3 to 1:6. It seems like casing layer doesn’t really impact results.

Can anyone offer any advice or tips on navigating both the regular Natalensis and the NSS varieties?

Thank you!

Peace & Light,

Seeker604


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OfflineBlueAndOrange
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Re: Psilocybe natalensis [Re: Seeker604] * 2
    #28176109 - 02/07/23 11:23 AM (1 year, 8 days ago)

I’m not sure there is a real distinction between nss and Natalensis as strains per se. That said, my success with nats has been to take a wispy agar plate to grain, then expand it doing G2G. Put it in shoeboxes using Dino soil (I get a lot of flak for using pre-prepped substrate, but I’ve also got only so much time to spare and hate this part) and spawn it 1:1 in a shoebox with a 0.5qt pseudo casing layer.

I’ve grown 70 shoeboxes and get between 1.5 and 2 oz dried per myco quart of spawn and the latest batch was tested above 21mg/g of psilocybin and above 24mg/g.

I grow it in temps between 60-65 degrees F and a not humid environment.

I never mist or mess with airflow. Just spawn and leave them until harvest. I typically get 3 flushes before I toss them.

I never get overlay.

All from a single drop of a MSS.


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InvisibleSeeker604
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Re: Psilocybe natalensis [Re: BlueAndOrange]
    #28176129 - 02/07/23 11:39 AM (1 year, 8 days ago)

Quote:

BlueAndOrange said:
I’m not sure there is a real distinction between nss and Natalensis as strains per se.




Yoshi’s site sells three Psilocybe Natalensis varieties to date:  Natalensis Super Strain (caps stain green), regular Natalensis, and Natalensis Low Spore. All three exhibit different phenotypes but are all Ps. Natalensis. The early runs by AKA and Crackatoa had results that match NSS with the green staining.

Quote:

BlueAndOrange said:
That said, my success with nats has been to take a wispy agar plate to grain, then expand it doing G2G. Put it in shoeboxes using Dino soil (I get a lot of flak for using pre-prepped substrate, but I’ve also got only so much time to spare and hate this part) and spawn it 1:1 in a shoebox with a 0.5qt pseudo casing layer.

I’ve grown 70 shoeboxes and get between 1.5 and 2 oz dried per myco quart of spawn and the latest batch was tested above 21mg/g of psilocybin and above 24mg/g.

I grow it in temps between 60-65 degrees F and a not humid environment.

I never mist or mess with airflow. Just spawn and leave them until harvest. I typically get 3 flushes before I toss them.

I never get overlay.

All from a single drop of a MSS.




Thank you very much for sharing your current process!

I’ll be logging my runs in my LAGM journal.

Peace & Light,

Seeker604


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OfflineSingularFusion
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Re: Psilocybe natalensis [Re: Seeker604] * 3
    #28176131 - 02/07/23 11:42 AM (1 year, 8 days ago)

I don't have any fruit in hand yet, but from the small work I have done so far, I can concur somewhat on silky myc being better in the tub, much less puffy overlay from those

silky low profile myc


puffy tomentose looks like cube myc


it's hard to even see how bad that overlay on Nat A is in a photo, it's almost as thick as the substrate itself, about two inches below all that is the surface somewhere. That was plain coir, I will try some more with manure to see if anything special happens. I guess cultural selection is important with these if you want great results :shrug: hoping it will still fruit some though, I see from this thread they usually fruit right through that mess. Look forward to trying some eh

Will report back one way or the other


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InvisibleGuerrilla
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Re: Psilocybe natalensis [Re: SingularFusion] * 3
    #28176138 - 02/07/23 11:50 AM (1 year, 8 days ago)

This comes up a lot and I just want to clarify that tomentose vs rhizomorphic has absolutely nothing to do whether your culture will overlay or not

I've never had overlay and have always used only heavily rhizomorphic and aggressive growth


--------------------
Being pissed on does not make you a real man.


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OfflineSingularFusion
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Re: Psilocybe natalensis [Re: Guerrilla]
    #28176139 - 02/07/23 11:51 AM (1 year, 8 days ago)

Fair enough man, duly noted :cheers:


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OfflineRescueU
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Re: Psilocybe natalensis [Re: Hillbillyjohn] * 2
    #28176147 - 02/07/23 11:56 AM (1 year, 8 days ago)

I nocced up 4 more jars, these were WBR. I'm trying for edible mycelium with these. Will try them at different months to see if the strength increases. Will also try to make 2 shoeboxes with WBR as the sub, get a few flushes of fruits and then try the sub to see how it compares.


--------------------
UT ALII VIVANT


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InvisibleShrimps
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Re: Psilocybe natalensis [Re: SingularFusion] * 1
    #28176153 - 02/07/23 12:03 PM (1 year, 8 days ago)

Quote:

Nef said:
I don't have any fruit in hand yet, but from the small work I have done so far, I can concur somewhat on silky myc being better in the tub, much less puffy overlay from those

silky low profile myc


puffy tomentose looks like cube myc


it's hard to even see how bad that overlay on Nat A is in a photo, it's almost as thick as the substrate itself, about two inches below all that is the surface somewhere. That was plain coir, I will try some more with manure to see if anything special happens. I guess cultural selection is important with these if you want great results :shrug: hoping it will still fruit some though, I see from this thread they usually fruit right through that mess. Look forward to trying some eh

Will report back one way or the other





You have the same cultre as I do (you got the other half of the print :laugh: ) And I can say, it makes beautiful fruits. Also made a clone, so if yours have problems, I can send you a slant later if you want ;-)


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

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OfflineSingularFusion
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Re: Psilocybe natalensis [Re: Shrimps]
    #28176162 - 02/07/23 12:15 PM (1 year, 8 days ago)

:hatsoff:

Thanks brother, hopefully it won't come to that... Sure she will do her thing, it hasn't been that long yet that I am really concerned, just never seen something get so stupid like that. The puffy stupid one really looks a lot like the one you grew also, even down to how it sees to prefer to overlay round the edges the most

I have to check my notes as well, if the plate gives no clue as to the outcome then perhaps I did something different with ratio or compression on that tub


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InvisibleShrimps
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Re: Psilocybe natalensis [Re: SingularFusion] * 2
    #28176164 - 02/07/23 12:18 PM (1 year, 8 days ago)

I compressed the living shit out of my nat trays when I spawned  them  xD


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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Offlinecozmyc
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Re: Psilocybe natalensis [Re: Shrimps] * 3
    #28176175 - 02/07/23 12:30 PM (1 year, 8 days ago)

Compressed bottle gang!


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You're conscious population 2 stardust
----------------------
and that's valuable :sunny:


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InvisibleSeeker604
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Re: Psilocybe natalensis [Re: Guerrilla]
    #28176176 - 02/07/23 12:31 PM (1 year, 8 days ago)

Quote:

Guerrilla said:
This comes up a lot and I just want to clarify that tomentose vs rhizomorphic has absolutely nothing to do whether your culture will overlay or not

I've never had overlay and have always used only heavily rhizomorphic and aggressive growth




With all due respect, if you take the time to read this thread from the beginning, you will see the overlay issues they encountered and that using tomentose led to fewer issues with overlay.

What spawn to sub ratio are you using for aggressive rhizo grows? 

Peace & Light,

Seeker604


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Offlinecozmyc
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Re: Psilocybe natalensis [Re: Seeker604] * 3
    #28176182 - 02/07/23 12:34 PM (1 year, 8 days ago)

You can run your cultures on small pf Pucks to see what they will do before jumping to grain


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You're conscious population 2 stardust
----------------------
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OfflineDERRAYLD
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Re: Psilocybe natalensis [Re: Seeker604] * 2
    #28176204 - 02/07/23 12:50 PM (1 year, 8 days ago)

Quote:

Seeker604 said:
Quote:

Guerrilla said:
This comes up a lot and I just want to clarify that tomentose vs rhizomorphic has absolutely nothing to do whether your culture will overlay or not

I've never had overlay and have always used only heavily rhizomorphic and aggressive growth




With all due respect, if you take the time to read this thread from the beginning, you will see the overlay issues they encountered and that using tomentose led to fewer issues with overlay.

What spawn to sub ratio are you using for aggressive rhizo grows? 

Peace & Light,

Seeker604




You may not know but Guerrilla has grown Natalensis a lot.

We have different Natalensis, I never get overlay and have a rhizo cultures.


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OfflineDERRAYLD
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Re: Psilocybe natalensis [Re: DERRAYLD] * 6
    #28176209 - 02/07/23 12:53 PM (1 year, 8 days ago)



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InvisibleShrimps
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Re: Psilocybe natalensis [Re: DERRAYLD]
    #28176232 - 02/07/23 01:09 PM (1 year, 8 days ago)

I hate your postservice xD But still hoping :smile: Got an other Nat culture from Egon aswell, which I am growing now in my LAGM. Courious if that one is also so overlayed. But the ones I grew now, hat beautiful small sized caps and really heavy bodys. Actually one of the most beautiful shrooms I've grown for a while, just the looks of it are like amazing :laugh:
Can't wait to try them in Autmn ^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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Offlinechris77
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Re: Psilocybe natalensis [Re: Shrimps] * 4
    #28176263 - 02/07/23 01:33 PM (1 year, 8 days ago)

the green cap is environment-related  and not a genetic trait as in a phenotype. i see it as related to humidity levels.
that's my opinion and experience anyway


--------------------
the observer is the observed
            j. krishnamurti


Edited by chris77 (02/07/23 01:33 PM)


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