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 mr boom
Stranger
Registered: 01/07/23
Posts: 4
Last seen: 1 year, 2 months
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 Wood ear clone - first post :) 
#28129340 - 01/07/23 01:48 PM (1 year, 3 months ago) |
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Hi all!
I’m new here  excited for my first post! ( Noobish - recently got into mycology)
So I found some wood ear fungus at my local dog park on a wood stump it was growing awesomely and I yoinked some and put it in a paper bag to take it home have a go at cloning it.
Upon opening the paper bag some hours later I noticed that there was a lot of white powdery stuff on the paper bag when lifting the wood ear.
I took 4 samples from the wild wood ear;
1. A wedge of the wood ear (cleaned with isopropyl alcohol then halved it horizontally - took one side of the ear) flesh touching the agar and skin membrane facing upwards.
2. A swab of the white powdery stuff - which I assume is spores.
3. There was a really thick area at the base that was full jelly flesh - heated a scalpel red hot and cut out a cube piece.
4. A small flesh piece from the base of another cluster of wood ear (different from no. 3)
These are the results:
1. 
2, 3, 4. 
2. (White powdery spores?) 6 o’clock - bottom left
3. (Cube piece) 10 o’clock - top left
4. (Small flesh piece) 2 o’clock - top right
At first the spores (2) had most activity and the cube (3) and the small flesh (4) had a bit of contam and bacteria on it. The wedge had a little bit of contam as well but had a bit of good (I assume) activity happening. This was close to Christmas and New Years and I was away from observing it for at least a week and a half and came back and surprisingly saw that the cube (3) grew a massive ball of mycelium even though it had contam to begin with.
The wedge (1) was interesting as well it looked rooty.
I took transfers from the cube sample (3) and a clear area which looked like it had mycelium growth in the agar - not on the surface.
Some of the white mycelium from the cube (3) had contam / bacteria but more than half did not ( which is awesome ) and the agar that had the mycelium growth [near the centre] ( I assume is mycelium growth ) grew quite differently - not sure if that is what I’m after or if it is just mould or bacteria contam - but it’s growing like mycelium.
QUESTIONS FOR YOU ALL WHO WOULD LIKE TO INPUT
Does anyone know what that growth is near the centre? Is that mycelium growth?
It seems to be growing at a quicker rate than the white mycelium.
The white mycelium is what I should be after right? It grew weirdly it and didn’t really spread thinly out - it grew out thickly compared to what I have seen on past posts on here and online.
Keen to know anyones thoughts on my clone attempt.
Cheers!
Alan
Edited by mr boom (01/07/23 01:49 PM)
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dyel
 
Registered: 10/15/21
Posts: 920
Last seen: 1 month, 23 hours
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 Re: Wood ear clone - first post :) [Re: mr boom]
#28130329 - 01/08/23 09:07 AM (1 year, 3 months ago) |
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hello, I'm not familiar with this species mycelium but the growth near the center looks like mold to me
maybe someone else can provide more helpful info
welcome to the shroomery
edit: after searching old post I'm confused now
https://www.shroomery.org/forums/showflat.php/Number/9458669
Edited by dyel (01/08/23 09:14 AM)
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mr boom
Stranger
Registered: 01/07/23
Posts: 4
Last seen: 1 year, 2 months
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Re: Wood ear clone - first post :) [Re: dyel]
#28131493 - 01/08/23 09:42 PM (1 year, 3 months ago) |
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At first I thought it may be mould but it kind of grows like mycelium.
I was hoping someone familiar with this species may be able to shed some light on this situation. The sample as mentioned was taken from the same plate in an area that was clear of bacteria but looked like mycelium was growing throughout the agar below the surface level.
The mycelium ( white ) is growing rather interestingly as it’s staying dense as it spreads rather than branching out.
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mr boom
Stranger
Registered: 01/07/23
Posts: 4
Last seen: 1 year, 2 months
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Re: Wood ear clone - first post :) [Re: mr boom]
#28131498 - 01/08/23 09:46 PM (1 year, 3 months ago) |
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Updated pics from today.
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dyel
Registered: 10/15/21
Posts: 920
Last seen: 1 month, 23 hours
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Re: Wood ear clone - first post :) [Re: mr boom]
#28144331 - 01/17/23 11:48 AM (1 year, 2 months ago) |
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I'm curious about if you managed to clean it up
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TravelAgency
The ongoing "wow"
Registered: 12/25/10
Posts: 4,431
Last seen: 1 year, 2 months
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Re: Wood ear clone - first post :) [Re: mr boom]
#28155697 - 01/24/23 02:50 PM (1 year, 2 months ago) |
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Quote:
mr boom said:
Updated pics from today.
Looks like mold to me. I tried to clone wood ear in the past and it's a bitch
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 alaskappalachian
Entitiologist
Registered: 10/22/19
Posts: 1,758
Loc: The 49th Dimension
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Re: Wood ear clone - first post :) [Re: TravelAgency]
#28156424 - 01/25/23 12:40 AM (1 year, 2 months ago) |
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Edit: it was witch's butter I tried to grow now that I recall. Disregard   Best of luck though!
--------------------
THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES
ISO:Gym punctifolius spores for trade 
Edited by alaskappalachian (01/25/23 02:25 AM)
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mr boom
Stranger
Registered: 01/07/23
Posts: 4
Last seen: 1 year, 2 months
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Re: Wood ear clone - first post :) [Re: dyel]
#28175668 - 02/07/23 01:43 AM (1 year, 2 months ago) |
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Here is an update -
I made a liquid culture from some of the mycelium from the agar plate and it turned out great 
Inoculated grain from liquid culture;
It’s seems to have a darker appearance or has blackened the grain which is pretty cool - Here is a lions main jar I made at the same time you can see that it’s not as black
Any suggestions or tips for substrate mix for the wood ear?
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dyel
Registered: 10/15/21
Posts: 920
Last seen: 1 month, 23 hours
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Re: Wood ear clone - first post :) [Re: mr boom]
#28175683 - 02/07/23 02:24 AM (1 year, 2 months ago) |
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as i mentioned before I have no experience with this species but afaik this is a woodlover so maybe some hwfp mix? what do you usually use for other woodlover species?
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Bonita1Flakes
Registered: 05/07/23
Posts: 23
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Re: Wood ear clone - first post :) [Re: mr boom]
#28309951 - 05/07/23 06:35 AM (11 months, 4 days ago) |
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Just found a video and researchgate article
Biology and Cultivation of Black Ear Mushroom – Auricularia spp
https://www.researchgate.net/publication/315727943_Biology_and_Cultivation_of_Black_Ear_Mushroom_-_Auricularia_spp
Cultivation in Compost
Raw materials
Sawdust -78%
Rice bran- 28%
White sugar-1%
Calcium carbonate-1%
Composting of sawdust for Auricularia cultivation usually takes five days to produce the type of compost required. Mix thoroughly the weighed raw materials indicated above and maintain moisture level at 65-70%.
Pile the material in pyramid shape, turnings should give at 2 days interval then supplements are added.
After completing compost preparation, sterilized and allowed to inoculation and fruiting body formation of Auricularia
Rubber saw dust when used as a substrate for the growth of Auricularia gave maximum yield of 180.67g in 3 harvests
The Falcataria. moluccana is the most suitable tree spp. that can be used for making the substrate for cultivation
Composted substrates supplemented with 10% wheat bran supported higher yields in any substrates
Grow Wood Ears Using Bagged Wood
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kedi
people are strange
Registered: 01/14/23
Posts: 48
Last seen: 9 months, 24 days
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Re: Wood ear clone - first post :) [Re: Bonita1Flakes]
#28314013 - 05/10/23 09:40 AM (11 months, 22 hours ago) |
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wow huge 2 person operation. i collect wood ear (judas) from elder.
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Bonita1Flakes
Registered: 05/07/23
Posts: 23
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Re: Wood ear clone - first post :) [Re: kedi]
#28347331 - 06/04/23 05:12 PM (10 months, 6 days ago) |
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👍Even better! Free cloud ears. Trying to grow them soon
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