|
 Phaedra
Mycolobster
Registered: 03/25/15
Posts: 179
Loc: N. America
|
 365nm light and mycelium. Interesting Observation. *DELETED*  1
#28159739 - 01/27/23 11:36 AM (1 month, 26 days ago) |
|
|
Post deleted by Phaedra
Reason for deletion: X
|
 Alan Rockefeller
Mycologist
 
Registered: 03/10/07
Posts: 47,777
Last seen: 17 hours, 15 minutes
|
Re: 365nm light and mycelium. Interesting Observation. [Re: Phaedra] 1
#28171429 - 02/03/23 11:10 PM (1 month, 19 days ago) |
|
|
I wonder if you have a couple different species growing in that first plate. Would be cool to see another set of pictures now that a week has passed.
|
nektar61
Tripping frog whisperer
 
Registered: 07/04/20
Posts: 2,778
Loc: CubeSat in low earth orbit
Last seen: 18 hours, 54 minutes
|
Re: 365nm light and mycelium. Interesting Observation. [Re: Phaedra]
#28172642 - 02/04/23 08:39 PM (1 month, 18 days ago) |
|
|
To anyone reading this: 365nm UV light requires specific protective glasses to protect your eyes, and you should keep it off your skin, and keep pets and other people out of the room. The cheaper glasses offered for this might be bunk too, that's a thing. Get the right ones from a name science store or rock collecting store, not cheap of Amazon.
Even with the right glasses, the better 365nm UV lamps have a quartz shield to keep other harmful rays out of the room that could harm you and also skew your results.
I posted on agar envy, I'll repost that here. But also make the suggestion of growing some non-active mushroom cultures to see if what you're seeing is just a mushroom thing and not an actives thing.
Though I'm not sure it's even a mushroom thing:
Do you believe you're seeing active alkaloids fluorescing? Or different species?
Pretty sure any glow you see on the culture there is mostly, or all, reflected off the florescence that is absolutely coming off the plate itself. and maybe some from whatever you taped the plate shut with. And I'm seeing more of this reflected (or coming off) the part of culture that's thicker than the other part.
Thicker is going to have different reflection (or production) regardless of alkaloids or species. Try the same thing with a napkin, then a napkin folded over twice and see if there's a difference.
Try it again with empty plates (or better yet poured plates that you haven't nocked yet) taped shut and on the same background.
Also repeat this photo when you take the lid off to transfer. The lid is going to influence this "experiment."
One of my first posts here, 28 months ago, was me thinking I could detect contam using UV light:
https://www.shroomery.org/forums/showflat.php/Number/26942242
Now I think I was just seeing slight florescence in the jar, grain, or plate itself.
Any actual experiments should be done with clones that you've grown too, in case there's something in multispore that causes a difference in what you think is something else.
I dig the curiosity though.
Edited by nektar61 (02/04/23 09:50 PM)
|
Alan Rockefeller
Mycologist
Registered: 03/10/07
Posts: 47,777
Last seen: 17 hours, 15 minutes
|
Re: 365nm light and mycelium. Interesting Observation. [Re: nektar61] 1
#28174090 - 02/05/23 09:26 PM (1 month, 17 days ago) |
|
|
Quote:
nektar61 said:
To anyone reading this: 365nm UV light requires specific protective glasses to protect your eyes, and you should keep it off your skin, and keep pets and other people out of the room. The cheaper glasses offered for this might be bunk too, that's a thing. Get the right ones from a name science store or rock collecting store, not cheap of Amazon.
Even with the right glasses, the better 365nm UV lamps have a quartz shield to keep other harmful rays out of the room that could harm you and also skew your results.
I think you are confusing 365 nm with 254 nm - 365 is much safer, it's 254 that I use glasses and subscreen with. 365 works a whole lot better for fluorescent photography so I don't use the 254 much, those are mostly used for minerals. I wouldn't shine 365 in my eyes but it's probably fine to shine off into the woods.
You can test glasses by shining the UV light through them at something fluorescent like bleached paper. All of the glasses I have tested block it very well.
Quote:
Do you believe you're seeing active alkaloids fluorescing? Or different species?
It's likely the beta-carbolines which have MAOI activity.
A cool thing to do is dissolve some mycelium or mushroom in 70% alcohol in a glass vial and check the fluorescence of the liquid. You get much purer colors this way without all of the mushroom cells in the way.
|
nektar61
Tripping frog whisperer
Registered: 07/04/20
Posts: 2,778
Loc: CubeSat in low earth orbit
Last seen: 18 hours, 54 minutes
|
Re: 365nm light and mycelium. Interesting Observation. [Re: Alan Rockefeller] 1
#28174228 - 02/05/23 10:37 PM (1 month, 17 days ago) |
|
|
Quote:
Alan Rockefeller said:
It's likely the beta-carbolines which have MAOI activity.
A cool thing to do is dissolve some mycelium or mushroom in 70% alcohol in a glass vial and check the fluorescence of the liquid. You get much purer colors this way without all of the mushroom cells in the way.
Thank you for the answers, Alan.
I worry about cheap "special" lights mass-produced in China emitting wavelengths outside what's specified. I know it's a big problem with a lot of cheap green laser pointers, many of them produce IR laser too, which you can't see, and can harm your eyes, it also might reflect at a different angle due to chromatic aberration in cheap focusing lenses. I'll take your word for it that that wavelength is safe, but I wear goggles with mine anyway, because of that.
Do you think there is florescence in those plates' contents? It looks to me like the the plastic in the plates themselves is fluorescing, and what's showing on the culture is largely reflection of that. It's not bright like with your recent spore print, for instance.
I mentioned to the OP elsewhere that I'd love to see a control group, a poured sealed plate not inoculated, just a blank plate of same type, shot the same way to see the difference.
I did some tests like that on my end, posted below.
I didn't have a blank plate for a control, but I had a plate I put some noc'ed grains on yesterday, same plates, same agar, same pour, hasn't grown noticeably yet.....vs a plate with some cube mycelium growing on it. My UV flashlight is 395 nm, is that OK? Or do I need 365 for this? does 395 need protective glasses? This is the flashlight I used:
https://www.amazon.com/Escolite-Flashlight-Ultraviolet-Blacklight-Detector/dp/B008133KB4?th=1
I tried photographing both at the same time but couldn't get them both lit well. I did this on a black background as did OP, did it in a completely dark room with a DSLR and did no post processing except cropping.
My results seemed to show MAYBE a TINY bit of purple florescence on the mycelium (could just be reflection of the visible purple in the light), but also some green florescence from the plate's plastic. The plates are clear with no green in white light. I'd call mine inconclusive.
 
Edited by nektar61 (02/13/23 07:56 PM)
|
Phaedra
Mycolobster
Registered: 03/25/15
Posts: 179
Loc: N. America
|
Re: 365nm light and mycelium. Interesting Observation. *DELETED* [Re: nektar61] 1
#28174349 - 02/06/23 02:44 AM (1 month, 17 days ago) |
|
|
Post deleted by Phaedra
Reason for deletion: X
|
nektar61
Tripping frog whisperer
Registered: 07/04/20
Posts: 2,778
Loc: CubeSat in low earth orbit
Last seen: 18 hours, 54 minutes
|
Re: 365nm light and mycelium. Interesting Observation. [Re: Phaedra]
#28175218 - 02/06/23 06:23 PM (1 month, 16 days ago) |
|
|
Quote:
Phaedra said:
It’s for sure fluorescence.
I think you're right, that you're seeing some with the mycelium, and is probably what Alan said.
But I also still think you're getting some florescence from the plate. Did you see the pics I posted above? Was an edit hours after I wrote the post.
Took pix of two plates, one with mycelium, one with none, both have a bit of green florescence from the plate on a plate that's clear in white light.
I like Alan's idea of a shroom extract to see more florescence without the fungus cells in the way. I'll try that.
Cheers.
|
sporemaster420
Stranger
Registered: 02/11/23
Posts: 27
Last seen: 8 hours, 11 minutes
|
Re: 365nm light and mycelium. Interesting Observation. [Re: nektar61]
#28211596 - 03/02/23 07:44 PM (23 days, 12 hours ago) |
|
|
Funny coincidence, I just read that a few milliseconds of particular wavelengths of light are enough to induce fruiting conditions. I believe it was in Stamets book. I happen to have a powerful 365nm UV flashlight. Cost me like $100 or so and now I finally have a use for it lol. I’m gonna try using it to induce fruiting conditions once my APEs are ready.
|
nektar61
Tripping frog whisperer
Registered: 07/04/20
Posts: 2,778
Loc: CubeSat in low earth orbit
Last seen: 18 hours, 54 minutes
|
Re: 365nm light and mycelium. Interesting Observation. [Re: sporemaster420]
#28214320 - 03/04/23 02:50 PM (21 days, 16 hours ago) |
|
|
Quote:
sporemaster420 said:
Funny coincidence, I just read that a few milliseconds of particular wavelengths of light are enough to induce fruiting conditions. I believe it was in Stamets book. I happen to have a powerful 365nm UV flashlight. Cost me like $100 or so and now I finally have a use for it lol. I’m gonna try using it to induce fruiting conditions once my APEs are ready.
Stamets says a lot of things, some are legit, some were guesses that didn't stand the test of time.
I think if additional UV light helped growth, everyone would be doing it.
FYI, sunlight is 3 to 5 percent ultraviolet light.
I'd recommend if you want to produce useful, scientific data that you do a double blind test, using same clone for both, not a multispore culture, as I said above to another poster.
I often say this on here when people suggest "experiments", people usually shout it down, but then the data is never presented.
I'd love to see some people actually do some of these experiments people suggest here from time to time. Almost no one does, and if they do, they almost never do a double blind using a clone.
If you're new here, I'd recommend just getting really good at growing shrooms with normal teks before trying to change the shroom world. A lot of us (including me) tried to run before we could walk here.
Welcome.
Edited by nektar61 (03/04/23 03:16 PM)
|
|
Threaded
Previous
Index
Next
Buy Bali Kratom Powder 
Buy Kratom Extract 
CBD Concentrates 
Injection Grain Bag
Edit 
Reply 
wikedanjel
1 2 all )
Baby_Hitler
Olgualion
Curious_George
MsPacMan