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San Pedro Girl
Shoebox Ninjaπ₯·




Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!π·
Last seen: 1 month, 28 days
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Re: Post Your New Tools Of The Trade. [Re: LogicaL Chaos] 2
#28173729 - 02/05/23 05:28 PM (1 year, 3 months ago) |
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Quote:
LogicaL Chaos said: Damn! The top of your SAB is so clear! Is that glass? 
Itβs a 3mm acrylic panel.
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Floret
Unmodify everything

Registered: 01/02/23
Posts: 275
Loc: underneath the water
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Your setup is so nice!
-------------------- LAGM 2024
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 70,093
Loc: The Inexpressible...
Last seen: 1 day, 23 hours
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Ballin! That was second guess
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 6 months, 19 days
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Re: Post Your New Tools Of The Trade. [Re: LogicaL Chaos] 3
#28174261 - 02/05/23 11:35 PM (1 year, 3 months ago) |
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SPG always has the coolest lab equipment. Speaking of which my metal petri sleeve should be here soon...
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dowodenum
Noob Pope



Registered: 08/05/19
Posts: 1,268
Loc: π¨π¦
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Re: Post Your New Tools Of The Trade. [Re: Greg] 6
#28174291 - 02/06/23 12:13 AM (1 year, 3 months ago) |
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Heated and bent this stainless straw (badly haha - stainless cools down so damn fast!!) to use as a punch with D3's tek. Repurposed the silicone mouthpiece for a heat-resistant handle, not that I really heat it often enough that heat becomes an issue.
The bend is about 45 degrees to keep my hand from being above the plate, and the 2 inch straight tip helps with deeper plates (jam jars, ziploc mini rounds).
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San Pedro Girl
Shoebox Ninjaπ₯·




Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!π·
Last seen: 1 month, 28 days
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Re: Post Your New Tools Of The Trade. [Re: dowodenum]
#28174544 - 02/06/23 08:10 AM (1 year, 3 months ago) |
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Quote:
dowodenum said:

Heated and bent this stainless straw (badly haha - stainless cools down so damn fast!!) to use as a punch with D3's tek. Repurposed the silicone mouthpiece for a heat-resistant handle, not that I really heat it often enough that heat becomes an issue.
The bend is about 45 degrees to keep my hand from being above the plate, and the 2 inch straight tip helps with deeper plates (jam jars, ziploc mini rounds).
How do you get the piece out after you punch?
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dowodenum
Noob Pope



Registered: 08/05/19
Posts: 1,268
Loc: π¨π¦
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Quote:
San Pedro Girl said: How do you get the piece out after you punch?
Blow in it? 
Naw I've never had it stick in the punch, done probably 40 transfers with it so far. Cool it in a sacrificial plate, then pop pop pop, perfect agar cylinders. Sometimes they'll come up a little and lay tilted as I pull the punch out, but that just makes it even easier!
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San Pedro Girl
Shoebox Ninjaπ₯·




Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!π·
Last seen: 1 month, 28 days
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Re: Post Your New Tools Of The Trade. [Re: dowodenum] 1
#28174614 - 02/06/23 09:20 AM (1 year, 3 months ago) |
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Quote:
dowodenum said:
Quote:
San Pedro Girl said: How do you get the piece out after you punch?
Blow in it? 
Naw I've never had it stick in the punch, done probably 40 transfers with it so far. Cool it in a sacrificial plate, then pop pop pop, perfect agar cylinders. Sometimes they'll come up a little and lay tilted as I pull the punch out, but that just makes it even easier!
So do they not stay in the straw? You just cut the circle with the straw and then transfer with a scalpel? Iβm trying to picture it.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
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It doesnt stay in the straw most times.
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dowodenum
Noob Pope



Registered: 08/05/19
Posts: 1,268
Loc: π¨π¦
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Re: Post Your New Tools Of The Trade. [Re: JHOVA] 1
#28174639 - 02/06/23 09:34 AM (1 year, 3 months ago) |
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Yep SPG. D3 has a good unlisted video in his thread
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 28 days
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Quote:
San Pedro Girl said:
Quote:
dowodenum said:

Heated and bent this stainless straw (badly haha - stainless cools down so damn fast!!) to use as a punch with D3's tek. Repurposed the silicone mouthpiece for a heat-resistant handle, not that I really heat it often enough that heat becomes an issue.
The bend is about 45 degrees to keep my hand from being above the plate, and the 2 inch straight tip helps with deeper plates (jam jars, ziploc mini rounds).
How do you get the piece out after you punch?
I built a fancy one last year that has a metal plunger rod on a spring. Photo below shows the fancy one on bottom. Photo on top is just more of the thin-walled stainless steel tubing I used to create the plunger-version. After using the plunger version a number of times, I put it away and haven't used it since, opting instead to just use the plain old stainless tube. Couple reasons for that - first is that the solid plunger absorbs a lot of heat each time your sterilize it so you have to wait longer for it to cool down and you have to be really sure it's cool before you use it otherwise it will kill the mycelium on your plug. Second is that 9 out of 10 punches I make on a plate result in the plug staying put on the plate so you then need to lift it out with a scalpel, and of the 1 out of 10 that come away with the punch, it's very easy to pop it out of the punch with a scalpel.
Here is the tubing. Heats fast and cools in under 10 seconds due to how thin it is: https://www.mcmaster.com/catalog/129/183/6100K356
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dowodenum
Noob Pope



Registered: 08/05/19
Posts: 1,268
Loc: π¨π¦
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Re: Post Your New Tools Of The Trade. [Re: Hindsight] 2
#28174678 - 02/06/23 09:59 AM (1 year, 3 months ago) |
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Wow yeah that is fancy. Maybe you just need one that can suck as well as blow!
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,171
Loc: United Kingdom
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Re: Post Your New Tools Of The Trade. [Re: dowodenum] 11
#28174684 - 02/06/23 10:01 AM (1 year, 3 months ago) |
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Ya boy gots a stainless steel table on the way
Never did I think I'd be excited to get a fucking table
-------------------- Being pissed on does not make you a real man.
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 10,170
Loc: South Africa
Last seen: 6 hours, 13 minutes
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Re: Post Your New Tools Of The Trade. [Re: Guerrilla] 5
#28174700 - 02/06/23 10:19 AM (1 year, 3 months ago) |
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Not even the periodic table?
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San Pedro Girl
Shoebox Ninjaπ₯·




Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!π·
Last seen: 1 month, 28 days
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Re: Post Your New Tools Of The Trade. [Re: Guerrilla] 2
#28174711 - 02/06/23 10:33 AM (1 year, 3 months ago) |
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Quote:
Guerrilla said: Ya boy gots a stainless steel table on the way
Never did I think I'd be excited to get a fucking table
Iβm so jealous right now. I really want to get rid of my wood desk, but hard to justify because it works just fine. When yours arrives, post pics and tell us about all the amazing benefits so I can finish rationalizing the purchase to myself.
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,171
Loc: United Kingdom
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I'll try not to tempt you too much with my shiny, smooth surface of steel
-------------------- Being pissed on does not make you a real man.
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milkboy
Child



Registered: 05/01/21
Posts: 2,389
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Re: Post Your New Tools Of The Trade. [Re: Guerrilla] 5
#28174767 - 02/06/23 11:19 AM (1 year, 3 months ago) |
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San pedro girl dont listen to them they are lying, that pucks gona get stuck every time, its a trap. Its conspirators working against you Im convinced they have anterior motives, I believe big steel has infiltrated mush cult.
-------------------- Gummies by milkboy Milkboys Public Display of Affection When the student is ready, the teacher appears
  
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
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Re: Post Your New Tools Of The Trade. [Re: milkboy] 4
#28174798 - 02/06/23 11:41 AM (1 year, 3 months ago) |
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All about them anterior motives.
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San Pedro Girl
Shoebox Ninjaπ₯·




Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!π·
Last seen: 1 month, 28 days
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Re: Post Your New Tools Of The Trade. [Re: JHOVA] 5
#28174802 - 02/06/23 11:45 AM (1 year, 3 months ago) |
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Quote:
JHOVA said: All about them anterior motives.

And posterior too, judging by your profile pic.π
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milkboy
Child



Registered: 05/01/21
Posts: 2,389
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Re: Post Your New Tools Of The Trade. [Re: JHOVA] 3
#28174833 - 02/06/23 12:09 PM (1 year, 3 months ago) |
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Quote:
JHOVA said: All about them anterior motives.

 Im at work give me a break
-------------------- Gummies by milkboy Milkboys Public Display of Affection When the student is ready, the teacher appears
  
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