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OfflineNoobShroob
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Can you go straight to substrate with agar?
    #28173983 - 02/05/23 08:45 PM (1 year, 11 months ago)

Title says all.
I understand an agar tray is small but if you made a full sized plate or something of that sort could you just put it straight to substrate after it has clean myc growth?

And that also brungs the question how much is a safe amount to spawn. If grain is just for surface area maybe splitting two agar plates throughout would have similar effects.

Also i just wondered, if i cloned two different fruits, or the same fruit, and tried to put them in a substrate would they fight or grow together.


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OfflineExcess Taters
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Re: Can you go straight to substrate with agar? [Re: NoobShroob]
    #28174014 - 02/05/23 08:59 PM (1 year, 11 months ago)

Grain = nutrition.
Sub = water retention

We use coir for it's non nutritious properties, it prevents contamination, because contamination cannot grow without a source of nutrition.

Grain food.  Sub water.

Edit

A plate of agar has almost no nutrition, and cannot lead to a healthy flush of mushrooms.  It's only slightly nutritious, if you fucked with the ratio could you grow from an agar plates?  Yep.


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If you've never grown mushrooms before, here's how you start.  First, follow the Updated PF Tek, put 4 holes in jar lids not 1, and use a water tub! 
The next move is Shoebox Tek. After that you move onto grains, agar, monotubs.  Agar is easy, just do it.
Other useful links - Picture guide for how things should look and proper surface conditions guide
Growing APE or PE?  P9 pseudo casing tek

Edited by Excess Taters (02/05/23 09:01 PM)

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OfflineNoobShroob
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Registered: 10/19/16
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Re: Can you go straight to substrate with agar? [Re: Excess Taters]
    #28174017 - 02/05/23 09:00 PM (1 year, 11 months ago)

Isnt agar nutrition? How does it grow on agar then
Or what if you used a manure based substrate instead of coir, would that have the nutrition?


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Edited by NoobShroob (02/05/23 09:03 PM)

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OfflineOldManRiver
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Re: Can you go straight to substrate with agar? [Re: NoobShroob] * 2
    #28174036 - 02/05/23 09:07 PM (1 year, 11 months ago)

Quote:

NoobShroob said:
Isnt agar nutrition? How does it grow on agar then




Agar itself is non-nutritious. The plates we use are about 2 to 4% some sort of carbohydrate, which is food.  Agar plates for fungi are actually designed to be low nutrition, to encourage the myc to spread.

Grain jars are intended to be high nutrition, which is why in contaminates easily.  The energy available to create fruits comes exclusively from the grain.

Substrate material provides water for the mycelium to use to produce fruits, which are done through water expansion into primordia cells. As mycelium  colonizes into the substrate, it's extending itself at an energy cost, which comes from the grain. 

In the scenario you describe, there is no energy available to do any of this.

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OfflineExcess Taters
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Re: Can you go straight to substrate with agar? [Re: OldManRiver]
    #28174038 - 02/05/23 09:08 PM (1 year, 11 months ago)

I was going to reply again but you've outclassed me with a wonderful explanation.  Well done!


--------------------
If you've never grown mushrooms before, here's how you start.  First, follow the Updated PF Tek, put 4 holes in jar lids not 1, and use a water tub! 
The next move is Shoebox Tek. After that you move onto grains, agar, monotubs.  Agar is easy, just do it.
Other useful links - Picture guide for how things should look and proper surface conditions guide
Growing APE or PE?  P9 pseudo casing tek

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OfflineNoobShroob
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Registered: 10/19/16
Posts: 504
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Last seen: 9 days, 5 hours
Re: Can you go straight to substrate with agar? [Re: OldManRiver]
    #28174087 - 02/05/23 09:25 PM (1 year, 11 months ago)

Makes sense, thank you both!


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Invisibledowodenum
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Re: Can you go straight to substrate with agar? [Re: NoobShroob]
    #28174308 - 02/06/23 12:38 AM (1 year, 11 months ago)

Check out MudaFuka's bottle tek where he preps substrates full of nutrients and directly inoculates them.


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InvisibleFishlegs
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Registered: 12/29/19
Posts: 51
Re: Can you go straight to substrate with agar? [Re: NoobShroob]
    #28174310 - 02/06/23 12:38 AM (1 year, 11 months ago)

if you want to try agar to 'substrate' you could mix substrate with some grain and sterilize in jars and drop agar in after and see how that goes :shrug:

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