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esotericbound
Inquisitive Soul



Registered: 06/13/19
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: Hindsight]
#28170867 - 02/03/23 02:35 PM (1 year, 11 months ago) |
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Hindsight, on your agar plate that transfer piece is very round... is that something you take time to cut little circle out of dish it came from for some reason? My transfers are always just little triangular wedges.
-------------------- "...If you have any kind of intellectual filter at all, you quickly can satisfy yourself that our best efforts are nothing more than half completed stories told around the campfire; we don't actually know what our predicament is." -Terence McKenna
 
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AspectOfTheCreator
Mastering the Art of Success


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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: esotericbound]
#28171729 - 02/04/23 07:35 AM (1 year, 11 months ago) |
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esotericbound
Inquisitive Soul



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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: AspectOfTheCreator]
#28171865 - 02/04/23 10:11 AM (1 year, 11 months ago) |
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thanks for that - gorgeous agar work!
-------------------- "...If you have any kind of intellectual filter at all, you quickly can satisfy yourself that our best efforts are nothing more than half completed stories told around the campfire; we don't actually know what our predicament is." -Terence McKenna
 
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LookinOut
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: esotericbound]
#28172089 - 02/04/23 01:32 PM (1 year, 11 months ago) |
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That’s not pan mycelium. It is Ps. Mexicana var Jalisco. It even says so in writing on your plates. It looks like Mexicana mycelium too. You got confused about the genus that you inoculated.
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esotericbound
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: LookinOut] 1
#28172158 - 02/04/23 03:09 PM (1 year, 11 months ago) |
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No, "Panaeolus Cyanescens Jalisco Mexico" is the description on the website, the order confirmation, and receipt.
-------------------- "...If you have any kind of intellectual filter at all, you quickly can satisfy yourself that our best efforts are nothing more than half completed stories told around the campfire; we don't actually know what our predicament is." -Terence McKenna
 
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ruawakeyet

Registered: 05/03/21
Posts: 1,862
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: LookinOut]
#28172606 - 02/04/23 08:07 PM (1 year, 11 months ago) |
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Quote:
LookinOut said: That’s not pan mycelium. It is Ps. Mexicana var Jalisco. It even says so in writing on your plates. It looks like Mexicana mycelium too. You got confused about the genus that you inoculated.
That was my first suspicion, and I made the same mistake with a member talking about Natal Pan Cyan. Once I searched it, I actually found a spore vendor selling Pan Cyan spores from Natal.
Same kind of thing with Pan Cyan Jalisco; it has even been crossed with other pans.
To me, unfortunately, it just looks like mold, but I'm not very experienced with Mexicana on agar.
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esotericbound
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: esotericbound] 2
#28173295 - 02/05/23 11:17 AM (1 year, 11 months ago) |
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T1 Pan plates look promising - all parameters the same except different agar recipe (1000mL H2O : 8g ME : 4g potato flakes : .4g peptone)



-------------------- "...If you have any kind of intellectual filter at all, you quickly can satisfy yourself that our best efforts are nothing more than half completed stories told around the campfire; we don't actually know what our predicament is." -Terence McKenna
 
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ruawakeyet

Registered: 05/03/21
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: esotericbound]
#28173327 - 02/05/23 11:41 AM (1 year, 11 months ago) |
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Those are looking VERY promising!
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AspectOfTheCreator
Mastering the Art of Success


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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: esotericbound]
#28173469 - 02/05/23 01:39 PM (1 year, 11 months ago) |
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Those look more like what I got.

Distinctive Pan myc

I'd recommend atleast one more transfer before grains.
Edited by AspectOfTheCreator (02/05/23 01:41 PM)
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esotericbound
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: AspectOfTheCreator] 1
#28173509 - 02/05/23 02:16 PM (1 year, 11 months ago) |
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What is reasoning for another transfer? If it appears I don't have an isolate on these plates, I can understand need for another transfer. I thought I read somewhere here that too much mycelium expansion (i.e. more transfers) decreases the tenacity of the mycelium and because of that, transfers are to be minimized, is this not correct?
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Baba Yaga
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: esotericbound] 1
#28173527 - 02/05/23 02:29 PM (1 year, 11 months ago) |
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You can send this to grain if it still looks alright after it grew out a bit further. Just make sure you have a couple of plates as a backup you can go back to or take a transfer at the same you send this to grain, keep the rest of the plates around......something along these lines.
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AspectOfTheCreator
Mastering the Art of Success


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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: esotericbound]
#28173967 - 02/05/23 08:28 PM (1 year, 11 months ago) |
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It may or may not be necessary. I just like making atleast 2 transfers away from the last contaminated plate.
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ruawakeyet

Registered: 05/03/21
Posts: 1,862
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Re: Panaeolus Cyanescens multispore growth on agar appearance? [Re: AspectOfTheCreator]
#28174216 - 02/05/23 10:27 PM (1 year, 11 months ago) |
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Those first plates may have just been a funky agar recipe, it can make mycelium do some really strange things.
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