Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1
OfflineGermanShaman
Stranger
 User Gallery
Registered: 01/05/18
Posts: 343
Last seen: 1 month, 13 days
Having issues with Agar work. Just can't seem to get any Myc!!!
    #28168737 - 02/02/23 09:12 AM (1 year, 1 month ago)

I am having some major issues with my agar work. I just cant seem to get anything to grow.

Here is my process:

Agar Receipes tried.

250ml Tap Water
4g Agar
7g LME

Sterilised at 15psi for 30 mins

Poured into glass /plastic petri dishes, in front of flow hood.
Plastic containers have been cleaned with isoprop, glass have been put in microwave for about 10 mins.

Take spore print and create spore syringe with sterilized distilleld water.

Add several drops from spore syringe to cooled down agar plates.
Seal up edges of glass dish with sarange wrap, the plastic ones just have their tops placed on.
Leave in room at normal room temp (about 21 Deg C) and wait.


I dont have issues with Trich any more since I built the Flow hood.

I just dont seem to get any growth at all even after a month of waiting. I have had some success with Agar work before but now nothing seems to be growing.

I'm also trying the following Agar recipes to see if that helps.

250ml Potato solution (made by boiling some potato peelings)
4g Agar
3g LME

Also trying:

250ml Potato solution
4g Agar
4g dextrose

Would welcome any advices on the matter.

Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 7,043
Loc: Rent free in your head
Last seen: 5 hours, 43 minutes
Trusted Cultivator
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman]
    #28168743 - 02/02/23 09:15 AM (1 year, 1 month ago)

Can we see some pictures?


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

Extras: Filter Print Post Top
OfflineGermanShaman
Stranger
 User Gallery
Registered: 01/05/18
Posts: 343
Last seen: 1 month, 13 days
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: SirPsycho]
    #28168759 - 02/02/23 09:25 AM (1 year, 1 month ago)

Here are some pics. Pics 1 - 7 from left to right.



Extras: Filter Print Post Top
InvisibleSimulatedMatrix33
Inter-dimensional Being
Other


Registered: 04/09/22
Posts: 529
Loc: Planet Kai. Hacker of the Matr...
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman]
    #28168767 - 02/02/23 09:28 AM (1 year, 1 month ago)

How old are your prints you are trying to germinate.


--------------------
My L.A.G.M 2023 grow log
Everything you’ve learned and been told is a lie. You are living in a  simulated reality called the game 23. The obstacle is for you to escape this matrix. Your conscience is stored in the Black Saturn cube, you need to find a way to escape now as Judgement day is coming. I live through the conscience minds of those who seek enlightenment through way of psychedelic. I’m the messenger brought down to your realm


Extras: Filter Print Post Top
OfflineGermanShaman
Stranger
 User Gallery
Registered: 01/05/18
Posts: 343
Last seen: 1 month, 13 days
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: SimulatedMatrix33]
    #28168771 - 02/02/23 09:29 AM (1 year, 1 month ago)

The age of the prints varies from a a few months to about 2-4 years.

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 9,839
Loc: South Africa
Last seen: 6 hours, 42 minutes
Trusted Cultivator
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman] * 1
    #28168788 - 02/02/23 09:40 AM (1 year, 1 month ago)

Your plates are all contaminated with serious bacterial bloom of some sorts.
What made you think those plates were clean?

You also don't need to make a spore syringe to use the prints, just streak them to agar directly.

Not sure why you don't have growth but based on your plates I'm not sure your prints are okay to be honest.

Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 7,043
Loc: Rent free in your head
Last seen: 5 hours, 43 minutes
Trusted Cultivator
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman] * 2
    #28168799 - 02/02/23 09:48 AM (1 year, 1 month ago)

That's all contaminated and this is why:

Quote:

GermanShaman said:
Plastic containers have been cleaned with isoprop, glass have been put in microwave for about 10 mins.





Your containers are not sterile to start with. Isopropyl is a sanitizer, it doesn't sterilize and  empty plates in a microwave also don't sterilize because glass doesn't absorb the energy from microwaves and isn't getting heated up


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

Extras: Filter Print Post Top
OfflineGermanShaman
Stranger
 User Gallery
Registered: 01/05/18
Posts: 343
Last seen: 1 month, 13 days
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: DERRAYLD]
    #28168802 - 02/02/23 09:51 AM (1 year, 1 month ago)

I have thrown these plates away now and made some more.

I have tried to scrap the prints to agar directly, that did not really produce any more success. I went to syringe so that I could dilute the spores down into about 20ml of water and therefore make the spore print go further.

I have also managed to take the spore syringe and just inoculate some 1% honey solution LC and managed to get some growth that way.

I am also trying several different prints from several different sources to see if that is the issue.  It does seem to be a bit hit or miss!!!!

Extras: Filter Print Post Top
OfflineGermanShaman
Stranger
 User Gallery
Registered: 01/05/18
Posts: 343
Last seen: 1 month, 13 days
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman]
    #28168803 - 02/02/23 09:52 AM (1 year, 1 month ago)

The glass does get very hot in the microwave.

In any case, could I sterlise the glass in the oven at 200 Deg C for about 20 mins?

Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery

Registered: 02/17/17
Posts: 4,727
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman]
    #28168807 - 02/02/23 09:54 AM (1 year, 1 month ago)

Quote:

GermanShaman said:
The glass does get very hot in the microwave.

In any case, could I sterlise the glass in the oven at 200 Deg C for about 20 mins?




https://www.shroomery.org/forums/showflat.php/Number/28006384

Follow the no pour tek for glass petris.


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 9,839
Loc: South Africa
Last seen: 6 hours, 42 minutes
Trusted Cultivator
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman] * 1
    #28168811 - 02/02/23 09:57 AM (1 year, 1 month ago)

Quote:

GermanShaman said:
I have thrown these plates away now and made some more.

I have tried to scrap the prints to agar directly, that did not really produce any more success. I went to syringe so that I could dilute the spores down into about 20ml of water and therefore make the spore print go further.

I have also managed to take the spore syringe and just inoculate some 1% honey solution LC and managed to get some growth that way.

I am also trying several different prints from several different sources to see if that is the issue.  It does seem to be a bit hit or miss!!!!




You need the point of a pin worth of spores, actually a lot less.

No need to dilute anything to make the print go further.

Follow the no pour tek that JHOVA posted, it's your best bet.

I'm also doubting your flowhood to be honest.

Extras: Filter Print Post Top
Invisiblebakedbeings
orbiter of truth
 User Gallery

Registered: 09/01/20
Posts: 4,218
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: SirPsycho] * 2
    #28168813 - 02/02/23 09:58 AM (1 year, 1 month ago)

:whathesaid:
you are pouring agar into plates coated with bacteria

Quote:

I have also managed to take the spore syringe and just inoculate some 1% honey solution LC and managed to get some growth that way



you are squirting bacteria into a jar of bacteria food

Quote:

I am also trying several different prints from several different sources to see if that is the issue.  It does seem to be a bit hit or miss!!!!



its not the prints

slow down, stop experimenting, follow trusted teks. i agree that no pour is a good option

Edited by bakedbeings (02/02/23 09:59 AM)

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 9,839
Loc: South Africa
Last seen: 6 hours, 42 minutes
Trusted Cultivator
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: bakedbeings] * 1
    #28168816 - 02/02/23 09:59 AM (1 year, 1 month ago)

:whathesaid:
That lc will be trash as well unfortunately.

Extras: Filter Print Post Top
OfflineHindsight
Mad Scientist
 User Gallery

Registered: 01/24/21
Posts: 2,706
Last seen: 11 months, 2 days
Trusted Cultivator
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman]
    #28168820 - 02/02/23 10:03 AM (1 year, 1 month ago)

I wouldn't go spore to LC. Success rate will be low. Need to get your agar working.

200C for 20 mins is fine for sterilization but you have to remember that you can't just do that, take them out, and walk them over to your hood. You have to wrap them in foil so they don't get contaminated between the oven and your hood.

I'm not sure how you are scraping spores onto your plate, but I don't suggest holding the print over the plate and scraping in such a way to let the dislodged spores fall into the plate. Instead, take a wire loop, sterilize it red hot in front of your hood, let it cool in the hood airflow, then rub it on your print to pick up some spores onto it then streak it across your agar plate. Put the lid on. Wrap it with parafilm or cling wrap.

The issue with making spore syringes is that you are FAR more likely to pick up bacteria from the print with the water, and then it can multiply in the syringe, ensuring that any drop or squirt of liquid from that syringe ends up creating a big blotch of bacteria on your plate. Not to say spore syringes are bad, but if you are having bacterial contam issues now, stay away from syringe use.

To reiterate what SirPsycho said, ISO doesn't sterilize anything. It kills most bacteria, but not all, and it does absolutely nothing for mold spores other than spread them around. Anything you pour into must be completely sterile.

Extras: Filter Print Post Top
OfflineGermanShaman
Stranger
 User Gallery
Registered: 01/05/18
Posts: 343
Last seen: 1 month, 13 days
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: Hindsight]
    #28168975 - 02/02/23 11:38 AM (1 year, 1 month ago)

I've used a scalpel that is flame sterilized to scrape the spores into a small glass jar that I then squirt some sterilized distilled water into.

All this is done in front of the flow hood. The syringes and needs have all been sterilized in the PC at 15psi.

Maybe I should give the innoc loop a try.

Extras: Filter Print Post Top
Offlinejohnukguy
Learning
Male User Gallery


Registered: 06/23/22
Posts: 2,423
Loc: Colorado Flag
Last seen: 4 months, 21 days
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: GermanShaman]
    #28177710 - 02/08/23 01:34 PM (1 year, 1 month ago)

Can you post a pic of your flowhood and give us the specs please?


--------------------
How to post pictures to shroomery TEK

Shroomery Trusted Cultivator And Member YouTube Channels.

β€œEvey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


Extras: Filter Print Post Top
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 1,852
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: johnukguy]
    #28177799 - 02/08/23 02:46 PM (1 year, 1 month ago)

While all the comments above are correct, do keep your eyes open. I see potential myc on half those plates.



This is from the 4th one.

Extras: Filter Print Post Top
Invisibleruawakeyet
 User Gallery
Registered: 05/03/21
Posts: 1,862
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: LadysKnight] * 1
    #28177810 - 02/08/23 02:57 PM (1 year, 1 month ago)

You could also try BRF/Verm pucks, just make sure you get them sterilized beforehand. I've personally seen a BRF puck germinate spores that refused to germinate on agar.

Extras: Filter Print Post Top
Invisiblebakedbeings
orbiter of truth
 User Gallery

Registered: 09/01/20
Posts: 4,218
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: ruawakeyet] * 2
    #28177869 - 02/08/23 03:37 PM (1 year, 1 month ago)

Quote:

ruawakeyet said:
You could also try BRF/Verm pucks, just make sure you get them sterilized beforehand. I've personally seen a BRF puck germinate spores that refused to germinate on agar.



TEAM PUCK!!!!

Extras: Filter Print Post Top
Offlinefiddle_head
Male User Gallery

Registered: 08/05/08
Posts: 2,203
Last seen: 6 days, 2 hours
Re: Having issues with Agar work. Just can't seem to get any Myc!!! [Re: johnukguy] * 1
    #28178138 - 02/08/23 06:42 PM (1 year, 1 month ago)

Quote:

johnukguy said:
Can you post a pic of your flowhood and give us the specs please?



:whathesaid:

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Can agar be microwaved? iudexk 1,798 8 08/30/01 01:09 PM
by blackout
* what is myc. piss? blissedout 5,418 7 12/11/04 11:50 AM
by hyphae
* Contam in Agar alt. MushroomFriend 1,771 5 12/28/04 03:13 PM
by MushroomFriend
* test tubes agar fungophiliac 3,179 8 06/19/20 09:41 PM
by gorilliam
* AGAR ENVY! (Anything and All things agar!)
( 1 2 3 4 ... 1280 1281 )
TormatoMFacebookDiscordReddit 552,665 25,612 03/26/24 08:12 PM
by trippytimes
* Did I just throw out good agar plates Roksolud 383 5 02/08/23 05:54 PM
by Roksolud
* Agar recipe work well for germinating these five spores? KingCobWeb 10,043 8 02/24/03 11:48 PM
by Anno
* I extended my penis 6" using AGAR KingCobWeb 2,532 2 02/22/03 11:43 PM
by KingCobWeb

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
365 topic views. 31 members, 192 guests and 92 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.01 seconds on 14 queries.