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BeefSupremeJr
Detritivore



Registered: 11/02/11
Posts: 8,678
Loc: 29.9792° N, 31.1342° E
Last seen: 3 minutes, 55 seconds
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shroob good job asking question. Now you see why i was hesistant to answer in the dms. i never say things im not sure on and this one ive never seen.
I think you just cut your losses and start some new tubs. maybe forget that whole culture and go back to mss on agar or even switch varieties.
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NoobShroob
Mushroom Connoisseur



Registered: 10/19/16
Posts: 504
Loc: I dont even know
Last seen: 9 days, 5 hours
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They seem to grow out as stems, from the top of the caps, slowly as the mushroom gets bigger and older it starts to grow the mycelium looking hats you can see in the pictures, and then there is the obvious weird wet standouts which are super weird. i see people having one or 2 but mine was like 70-90% and maybe surprisingly they all continued to grow and usually the hats with them but all seemed to live a full healthy life. The wet ones idk i got them out cuz wtf lol.
Also rosecomb to my knowledge is a mutation, is the oozing a mutation as well because i still dont know what has caused that then, the metabolites turned nastier after the flush from excess water or what causes metabolites to react that way that they multiplied 100x in 2 hours. seemingly mostly on the top layer as well. It also seems to me that it leaked from the mushroom itself and thats what caused the ooze on the sub so im still tryna figure out what caused all that.
so in your opinion none of this looks like contam then? just mutated mushrooms and a abnormal amount of metabolites lol
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Edited by NoobShroob (02/01/23 08:29 PM)
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NoobShroob
Mushroom Connoisseur



Registered: 10/19/16
Posts: 504
Loc: I dont even know
Last seen: 9 days, 5 hours
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Quote:
BeefSupremeJr said: shroob good job asking question. Now you see why i was hesistant to answer in the dms. i never say things im not sure on and this one ive never seen.
I think you just cut your losses and start some new tubs. maybe forget that whole culture and go back to mss on agar or even switch varieties.
For sure the more i learn the more it helps me and helps others. I tossed it after i saw it after i tried soaking it, looked too nasty and figured it was done for wanted it out of my area before it got worse, i want to try something other than b+ but am not sure what though.
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BeefSupremeJr
Detritivore



Registered: 11/02/11
Posts: 8,678
Loc: 29.9792° N, 31.1342° E
Last seen: 3 minutes, 55 seconds
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Re: Mushroom hat yellow and oozing? [Re: NoobShroob]
#28168135 - 02/01/23 08:38 PM (1 year, 11 months ago) |
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Golden teacher is a classic. i do well with hulualta, mazatapec and cambodian but i havent done cambo in a while.
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IllicitMango
The Forbidden Fruit



Registered: 10/26/22
Posts: 153
Last seen: 1 year, 5 months
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Im currently running Golden Teachers I love them, although they're the only kind I've grown so far.
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ruawakeyet

Registered: 05/03/21
Posts: 1,862
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Re: Mushroom hat yellow and oozing? [Re: NoobShroob]
#28168188 - 02/01/23 09:19 PM (1 year, 11 months ago) |
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Noob, when you talk about "flushing" it, what exactly are you doing?
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NoobShroob
Mushroom Connoisseur



Registered: 10/19/16
Posts: 504
Loc: I dont even know
Last seen: 9 days, 5 hours
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Re: Mushroom hat yellow and oozing? [Re: ruawakeyet] 2
#28168204 - 02/01/23 09:40 PM (1 year, 11 months ago) |
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Well i had a lot of aborts so i assumed it was from fluctuation in moisture/FAE issues. Maybe one or the other as well. Regardless i managed to get 3 "flushes" groups they came in, but i heard once it dries out and stops growing you can soak it for a bit to try to bring it back, growing had slowed down to nearly a stop with the exception of the last flush that was going and all the aborts i figured i may as well try as it seemed like the end anyways. Then the weird oozy balls threw me off track lol
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BeefSupremeJr
Detritivore



Registered: 11/02/11
Posts: 8,678
Loc: 29.9792° N, 31.1342° E
Last seen: 3 minutes, 55 seconds
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Re: Mushroom hat yellow and oozing? [Re: NoobShroob]
#28168700 - 02/02/23 08:36 AM (1 year, 11 months ago) |
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that sub doesnt owe you anything.
you might even try going back to mss on agar and get something different.
without seeing your tub its hard to know what wrong but id consider using a regukar tub like shoebox or a mono.
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the_chosen_one
So Called Sage


Registered: 09/11/06
Posts: 3,950
Loc: 1984
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Re: Mushroom hat yellow and oozing? [Re: NoobShroob] 1
#28168945 - 02/02/23 11:18 AM (1 year, 11 months ago) |
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Quote:
NoobShroob said: They seem to grow out as stems, from the top of the caps, slowly as the mushroom gets bigger and older it starts to grow the mycelium looking hats you can see in the pictures, and then there is the obvious weird wet standouts which are super weird. i see people having one or 2 but mine was like 70-90% and maybe surprisingly they all continued to grow and usually the hats with them but all seemed to live a full healthy life. The wet ones idk i got them out cuz wtf lol.
Also rosecomb to my knowledge is a mutation, is the oozing a mutation as well because i still dont know what has caused that then, the metabolites turned nastier after the flush from excess water or what causes metabolites to react that way that they multiplied 100x in 2 hours. seemingly mostly on the top layer as well. It also seems to me that it leaked from the mushroom itself and thats what caused the ooze on the sub so im still tryna figure out what caused all that.
so in your opinion none of this looks like contam then? just mutated mushrooms and a abnormal amount of metabolites lol
I can see things better now.. bigger screen today. Yeah, I still say Rosecomb, but something bacterial maybe as well. Rosecomb is typically caused by cleaning chemicals that contain some form of petroleum. The whole tub looks off though and I have never seen both at once.
My next theory would be a viral infection. Many attack the plant or mushroom RNA to weaken the immune system. Would explain the aborts (if not conditions like FAE) and the over all sickly look. An attack could also damage the RNA and manifest as something that looks like Rosecomb being that the mutations could be similar.
It's a tough call being that both can share the same results and we can't culture anything out to verify. Either way I wouldn't use culture or spores from them and would re-evaluate anything they may have come from. The more I look at it the more I lean towards viral.
As for using them.. people consume aborts all the time. Vert is safe, so is Rosecomb. Most of what we deal with is the same. I'd still recommend drying and making tea rather than eating them though. Better safe than sorry. Now there is always a chance of something we've never seen be it maybe slim. There's more cultivation than ever.. the world is changing and adapting all the time. I've never seen anyone have troubles ingesting questionable mushrooms in decades and I've been around, but eventually, someone is bound to be the first of The Last of Us.
-------------------- "Luck favors the observant." - Workman
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NoobShroob
Mushroom Connoisseur



Registered: 10/19/16
Posts: 504
Loc: I dont even know
Last seen: 9 days, 5 hours
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Quote:
the_chosen_one said: I can see things better now.. bigger screen today. Yeah, I still say Rosecomb, but something bacterial maybe as well. Rosecomb is typically caused by cleaning chemicals that contain some form of petroleum. The whole tub looks off though and I have never seen both at once.
te]I thoroughly cleaned everything, i suppose the grains were purchases as well as the substrate but i mean why would there be petroleum in either. The only cleaning ive used is 70% iso.
Quote:
the_chosen_one said: My next theory would be a viral infection. Many attack the plant or mushroom RNA to weaken the immune system. Would explain the aborts (if not conditions like FAE) and the over all sickly look. An attack could also damage the RNA and manifest as something that looks like Rosecomb being that the mutations could be similar.
It's a tough call being that both can share the same results and we can't culture anything out to verify. Either way I wouldn't use culture or spores from them and would re-evaluate anything they may have come from. The more I look at it the more I lean towards viral.
So how does viral differ from mold or standard bacteria, and it would affect its genetics too as in spores shouldnt be used even?
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lizardbrains
Registered: 07/20/24
Posts: 2
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Re: Mushroom hat yellow and oozing? [Re: NoobShroob]
#29077158 - 01/04/25 01:43 PM (12 days, 1 hour ago) |
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Thanks for posting this Shroob. I’m seeing exactly the same in a tub of Tidal Wave… ‘fuzzy ball tophat’, viscous fluid emerging from cap and stem. Removal of fruit and cutting stipe lengthwise huge quantities of translucent fluid from the hollow middle. Interior is discolored grey and looks to be breaking down. H2O2 spritz on the liquid inside foams like crazy.
This is the second set of monotubs that have exhibited this, sourced from multiple jars of LC inoculated from the same MS syringe. The 1st time it eventually hit most of the (very plentiful) fruit. Composted the lot. This too was a bumper first flush. Oh well. Just starting the agar journey and will attempt a biopsy transfer from a clean looking fruit and see what happens. Again thanks for posting.
@the_chosen_one: if this is viral should I even bother with plating or am I taking a risk of further spread? So far its only this LC source that’s shown it… Thanks!
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the_chosen_one
So Called Sage


Registered: 09/11/06
Posts: 3,950
Loc: 1984
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Re: Mushroom hat yellow and oozing? [Re: NoobShroob]
#29077267 - 01/04/25 03:32 PM (12 days, 5 minutes ago) |
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Quote:
NoobShroob said:
Quote:
the_chosen_one said: I can see things better now.. bigger screen today. Yeah, I still say Rosecomb, but something bacterial maybe as well. Rosecomb is typically caused by cleaning chemicals that contain some form of petroleum. The whole tub looks off though and I have never seen both at once.
te]I thoroughly cleaned everything, i suppose the grains were purchases as well as the substrate but i mean why would there be petroleum in either. The only cleaning ive used is 70% iso.
Quote:
the_chosen_one said: My next theory would be a viral infection. Many attack the plant or mushroom RNA to weaken the immune system. Would explain the aborts (if not conditions like FAE) and the over all sickly look. An attack could also damage the RNA and manifest as something that looks like Rosecomb being that the mutations could be similar.
It's a tough call being that both can share the same results and we can't culture anything out to verify. Either way I wouldn't use culture or spores from them and would re-evaluate anything they may have come from. The more I look at it the more I lean towards viral.
So how does viral differ from mold or standard bacteria, and it would affect its genetics too as in spores shouldnt be used even?
Viral sets up the rest so it's really hard to distinguish. I was going off mostly gut feel so take it with a grain of salt, but it's something to be aware of. Yes, many virus's carry through spores as well as horizontally. First I would keep growing it if it has personal value.. see if it transfers through spores. Keep it fairly isolated from everything else for a while to be safe. If it transfers horizontally then we pretty much caught it in the act. Same would apply to the spores. Not sure how I missed that post.. sorry about that.
-------------------- "Luck favors the observant." - Workman
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the_chosen_one
So Called Sage


Registered: 09/11/06
Posts: 3,950
Loc: 1984
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Re: Mushroom hat yellow and oozing? [Re: lizardbrains]
#29077281 - 01/04/25 03:39 PM (11 days, 23 hours ago) |
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Again, further observation would be needed. The main thing is don't share a suspect culture or spores. I would consider any expansion of said culture or spores to be experimental.
Sounds like you have at least a bacterial infection, but that could be a secondary infection caused by a potential virus.
-------------------- "Luck favors the observant." - Workman
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lizardbrains
Registered: 07/20/24
Posts: 2
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Appreciated. Yeah the bacterial infection could just be opportunistic. Going to dump the worst mono and grab the best fruit from the other for a horizontal on a few plates. I’ll keep an eye on the remaining mono for some spore but if the oozy/weepy takes off before any drops, bye-bye! Really don’t want this migrating into my very productive treasure coast or the PE I’ve been working on! Thanks again.
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the_chosen_one
So Called Sage


Registered: 09/11/06
Posts: 3,950
Loc: 1984
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Re: Mushroom hat yellow and oozing? [Re: lizardbrains]
#29077320 - 01/04/25 04:23 PM (11 days, 23 hours ago) |
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Yeah always better safe than sorry. 
I was so confused when I saw this again. Didn't realize it was over a year ago already and I could hardly remember my ramble.
-------------------- "Luck favors the observant." - Workman
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