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InvisiblefahtsterM
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Re: The OFFICIAL Stasis Storage Thread [Re: Nextcontestant] * 4
    #28161534 - 01/28/23 03:33 PM (11 months, 23 days ago)

Lids for drying out colonized grains for dry stasis

Ppl have also just left some kernels in the bottom of their qts (after securing the lid back on) and let them dry out that way but I hear it can take quite a while. 

I’m going to doing a lot of drying of cultures out in the future and probably simplify my lid set up so it’s just a small SHIP and use only two layers of tyvek with a widemouth 1/2 pint.. putting that in a bag of desiccant (damprid) with the larger lid and less barriers should speed things up by another week I’m thinking.  Right now I’m at about 3 weeks from hydrated to dry where I can store as is or load into swab packs to take up less space


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Re: The OFFICIAL Stasis Storage Thread [Re: bakedbeings] * 2
    #28162509 - 01/29/23 08:32 AM (11 months, 22 days ago)

Quote:

fossilshark said:
This method is really interesting, so you think the mycelium goes into stasis despite the presence of oxygen? So basically it isn't exclusively no-oxygen no-nutrient but can also just be caused by a lack of water?




Well there’s no visible growth after many months.. if there is cell division, it’s happening at a speed that isn’t noticeable after a certain point of dryness.  I just revived this mak 118 jar that’s looked exactly like this since the end of February




I think if you remove any part of those three, oxygen, food, water, you can get stasis or at least a degree of it.

That mak 118 is just about start knotting/pinning so we’ll see how it does.  You’d think after being dried out for 10 months it’d slow it down or wear out.  We shall see.  Ime, it does not


I actually already revived the clone once this season using a separate dried out jar but I was trying to laymix in a bag… didn’t work out so well and I had to remove the block from the container so fruit out the side pins.


So I kept it normal this time around as far as sub construction and I think it’ll be even better

But I also revived this fp+ culture that was dried for 8 months

Before..


After revival..


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Re: The OFFICIAL Stasis Storage Thread [Re: fahtster] * 4
    #28182962 - 02/11/23 07:45 PM (11 months, 9 days ago)

Quote:

fahtster said:
That mak 118 is just about start knotting/pinning so we’ll see how it does.  You’d think after being dried out for 10 months it’d slow it down or wear out.  We shall see.  Ime, it does not






Bag did aight.



The question is if it’s able to go 10 months dried out at room temp and come back and do that ^^^ what’s stopping it from going 10 more, or even more than that? 

I have dried out cultures in jars that are over a year old.  I can revisit this thread in a couple years and give those cultures a try at reviving.. see ya then :lol:


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Re: The OFFICIAL Stasis Storage Thread [Re: Screwup] * 3
    #28486326 - 09/29/23 12:01 AM (3 months, 28 days ago)

Getting to be about that time of year for me.  Started reviving some dried out grain cultures 8 days ago.  I GLC the dry grains and inject them into fresh grains.  I can (and sometimes do) go straight to qts but I like to test the cultures first in small jars just to be sure. 

Claketi (dried out April 2023)



PE x RW f7 clone (dried out March 2023)



Mak TP (this one is from March 2021)



Fp+ f8 clone (dried out April 2023)



Shit amazes me every time.  These new jars will be used to glc to qts for tubs.  When I made these new jars, I made a second one of each that will be dried out again for further storage


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