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side-eye
potate


Registered: 09/01/22
Posts: 55
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Re: The OFFICIAL Stasis Storage Thread [Re: VietboyX]
#28161281 - 01/28/23 12:16 PM (11 months, 23 days ago) |
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Great write up, been waiting for this!
What are you sterilizing the strips in after soaking, just any pp5 container?
Would using wood skewers still use the same process... soak to hydrate, pc, lay on a colonized plate, then into sterile distilled water?
-------------------- LAGM 2023
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side-eye
potate


Registered: 09/01/22
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Re: The OFFICIAL Stasis Storage Thread [Re: bakedbeings] 4
#28181951 - 02/11/23 08:50 AM (11 months, 9 days ago) |
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I dig it so I tried it.
I got some 5mL tubes with a rack, and wood stir sticks. Each one breaks into three or four pieces that fit in the tubes. Then I filled a bunch of the bottles with distilled water.


I soaked the stir sticks in the bottle for about 30 minutes, figured they needed a little more time than paper. Poured water out of the stir stick bottles, then PC'd everything in my IP for 45 minutes. Stir sticks onto a few plates. These pictures were taken two days apart, myc is already starting to get into the wood. Be sure all the broken pieces are ripped apart from each other, you can see how the Reishi plate has a big V shape that I didn't want to risk pulling apart.
 
Once this grows into the wood I'll drop em into the distilled water. I'll report back in 30 years to check if your tek actually works.
-------------------- LAGM 2023
Edited by side-eye (02/11/23 08:51 AM)
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side-eye
potate


Registered: 09/01/22
Posts: 55
Last seen: 1 month, 23 days
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Re: The OFFICIAL Stasis Storage Thread [Re: fahtster] 1
#28190459 - 02/16/23 04:09 PM (11 months, 4 days ago) |
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1
Alright so I got two plates' sticks in water.

I ran into a few problems on this one. First was I let the plates colonize the sticks a little too much, especially on the Reishi plate. I tried picking it up with tweezers, but it was so stuck to the plate that I lifted up the whole puck of agar out of the dish. I tried cutting it out but it still grabbed any agar underneath the stick. Reishi was by far the worst, but all of the sticks lifted a bunch of myc off the plate. Here's how colonized the plates were:

Second, the wood sticks float. These tubes are 5mL so pretty short, and when I let go of the stick it floated about halfway out of the tube. I used the lid to push them down as I capped em. Longer tubes/sticks may not be as bad, and paper may not be as bad either. I'm worried they picked up some bac hitting the rim of the tube, so I will give this another go soon with paper vs sticks.
Lastly, my fault, I dropped some sticks in the wrong tube of water. Not sterile so I had to toss those. Don't work high, kids.
-------------------- LAGM 2023
Edited by side-eye (02/16/23 04:10 PM)
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side-eye
potate


Registered: 09/01/22
Posts: 55
Last seen: 1 month, 23 days
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Re: The OFFICIAL Stasis Storage Thread [Re: SoFlown99] 1
#28299273 - 04/28/23 07:39 PM (8 months, 26 days ago) |
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Put a few more cultures into stasis today. PC'd 18 of these tubes, cut some long wedges, and dropped em in. Wrap, label, into the rack.

-------------------- LAGM 2023
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side-eye
potate


Registered: 09/01/22
Posts: 55
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Re: The OFFICIAL Stasis Storage Thread [Re: bongoman]
#28419032 - 08/03/23 04:48 PM (5 months, 22 days ago) |
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Quote:
bongoman said: Let's say I have some spore prints on hand that I don't intend to grow out in the new future.
Would it make sense to start up these spore prints on agar just to get some clean growth to then store using this method?
Would it better to preserve genetics using an agar wedge in water or as a spore print?
That's what I'm doing with all of my spore prints/swabs now. I don't plan on growing for a while, but I'm getting everything germed and put in stasis. Not only is it a great secondary backup, but I think it'll help to speed things up when I'm ready to grow again.
-------------------- LAGM 2023
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side-eye
potate


Registered: 09/01/22
Posts: 55
Last seen: 1 month, 23 days
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
#28419036 - 08/03/23 04:50 PM (5 months, 22 days ago) |
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Quote:
fossilshark said: Threw some Gymnopilus Purperatus and Gandalf in stasis today, decided to switch over to wood sticks as they are way easier to handle then paper.
I wonder how necessary the water even is?


How did you like this compared to paper, other than handling? Guess we won't know til revived. Any issue with the sticks floating when you drop em in?
-------------------- LAGM 2023
Edited by side-eye (08/03/23 04:57 PM)
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