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vanceen
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Flow hood - how long to run before use?
#28143164 - 01/16/23 03:55 PM (1 year, 2 months ago) |
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I tried to post this before but it never appeared. Sincere apologies if this is a duplicate.
To make a long story short, I've been trying to cultivate for a little over six months, with a dismal success rate.
I've had a little success (2 jars out of 16) with PF tek (both from spores and LC), but a 0% success rate with grain in jars or bags. Typically, the mycellium starts to grow and seems to do fine for a week or two, then growth stalls and everything goes green. (Presumably trichoderma.)
I've done OK with agar dishes, whether from spores or LC. Maybe about 80% success rate, with bacteria, trichoderma, or cobweb mold knocking out about 20%.
I've really put a lot into trying to create and work in a sterile environment. I've got a 10'x10' room, a room filter running 24/7, I fog the room with disinfectant spray before working, and my workspace and everything that goes into it is sprayed and/or wiped with 70% isopropyl. Syringe needles and scalpels are heated to red flame before use. I'm working in front of a small (12") flow hood, the one that Midwest sells with the acrylic extensions coming forward from the sides.
Reading around, I've found that it's recommended to start up the vent hood an hour or two before working. This I have not done, I always start it up right before use.
Here's the question: Would not running the flow hood an hour or two before working be expected to lead to very high contamination rates?
I even saw a 2mm worm living inside one of my agar dishes a couple of days after I had inoculated it! The only thing I can think of is that the flow hood is blowing crap, including worm eggs, during the first hour or two. Does this make sense to you all?
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Screwup
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Re: Flow hood - how long to run before use? [Re: vanceen]
#28143169 - 01/16/23 03:57 PM (1 year, 2 months ago) |
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How long are you PCIN everything?
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vanceen
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Re: Flow hood - how long to run before use? [Re: Screwup]
#28143252 - 01/16/23 04:42 PM (1 year, 2 months ago) |
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Quote:
Screwup said: How long are you PCIN everything?
LC and water I PC at 15 psi for 20 min.
Grain jars I PC at 15 psi for 90 min.
Agar plates I buy. I did make one batch (PC'd 15 min at 15 psi), but it turned out lumpy and was a pain to pour. I also bought the BRF jars for PC tek.
I've never seen any contamination in grain jars that I haven't inoculated.
Edited by vanceen (01/16/23 04:42 PM)
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Screwup
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Re: Flow hood - how long to run before use? [Re: vanceen]
#28143257 - 01/16/23 04:45 PM (1 year, 2 months ago) |
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Is it a real flow hood or one you made? Is it an old filter unit? Other people will be better able to probe what the issue is.
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BeefSupremeJr
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Re: Flow hood - how long to run before use? [Re: Screwup]
#28143261 - 01/16/23 04:49 PM (1 year, 2 months ago) |
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i dont have a hood but if a fucking worm egg got into a plate, theres something way wrong.
is this one of these boxfan/furnace filter jobs?
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vanceen
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Re: Flow hood - how long to run before use? [Re: BeefSupremeJr]
#28143277 - 01/16/23 05:00 PM (1 year, 2 months ago) |
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The flow hood is a Bonsai unit, sold by Midwest. It's only a few months old.
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aintlifegrand
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Re: Flow hood - how long to run before use? [Re: vanceen]
#28143282 - 01/16/23 05:02 PM (1 year, 2 months ago) |
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Sounds like a flow hood issue. Do you go digging in your garden then use really bad technique?
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Guerrilla
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Re: Flow hood - how long to run before use? [Re: aintlifegrand]
#28143290 - 01/16/23 05:05 PM (1 year, 2 months ago) |
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Scrubbing air is outdated and false information.
Switch on your hood, gather your supplies and it'll be good to go by time you start working.
Literally just a few minutes is plenty of time to get to work.
-------------------- Being pissed on does not make you a real man.
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Mycolorado
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Re: Flow hood - how long to run before use? [Re: vanceen]
#28143293 - 01/16/23 05:07 PM (1 year, 2 months ago) |
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How are you inoculating the agar and grain jars?
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vanceen
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Re: Flow hood - how long to run before use? [Re: Mycolorado]
#28144314 - 01/17/23 11:35 AM (1 year, 2 months ago) |
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Quote:
Mycolorado said: How are you inoculating the agar and grain jars?
The agar is inoculated by spores, LC, and by agar to agar transfer.
The jars are mostly LC (after testing the LC on agar), sometimes agar to grain.
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vanceen
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Re: Flow hood - how long to run before use? [Re: aintlifegrand]
#28144316 - 01/17/23 11:38 AM (1 year, 2 months ago) |
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Quote:
aintlifegrand said: Sounds like a flow hood issue. Do you go digging in your garden then use really bad technique?
No gardening.
I think I'm using fairly good technique. Of course, this thread is in hopes of finding ways to improve.
I described my technique in my original post. The only thing I left out (I think) is that I'm wearing nitrile gloves sprayed with isopropyl and also spraying my forearms.
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vanceen
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Re: Flow hood - how long to run before use? [Re: Guerrilla]
#28144319 - 01/17/23 11:39 AM (1 year, 2 months ago) |
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Quote:
Guerrilla said: Scrubbing air is outdated and false information.
Switch on your hood, gather your supplies and it'll be good to go by time you start working.
Literally just a few minutes is plenty of time to get to work.
If so, that's bad news for me. I would have no idea how to proceed except to go back to the SAB, which didn't give me great results either.
EDIT: I was thinking that running the hood before working might be effective not so much because it scrubs the air (I have a freestanding filter on all the time anyway). I was wondering whether it was blowing out crap that had settled on my filter between sessions.
Edited by vanceen (01/17/23 11:41 AM)
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donrjuan
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Re: Flow hood - how long to run before use? [Re: vanceen]
#28144392 - 01/17/23 12:30 PM (1 year, 2 months ago) |
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If you have been struggling with trich, it may be time for some heavy duty cleaning. Iso will not get rid of trich spores. Use a 10% bleach solution and scrub everything down, trays/tubs, work surfaces, tent if using, etc. Once you have had this many issues, you likely have spores one everything and they are STICKY!
Best of luck!
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vanceen
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Re: Flow hood - how long to run before use? [Re: donrjuan]
#28144430 - 01/17/23 01:10 PM (1 year, 2 months ago) |
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Quote:
donrjuan said: If you have been struggling with trich, it may be time for some heavy duty cleaning. Iso will not get rid of trich spores. Use a 10% bleach solution and scrub everything down, trays/tubs, work surfaces, tent if using, etc. Once you have had this many issues, you likely have spores one everything and they are STICKY!
Best of luck!
Thanks, that's helpful.
That's the first I've heard that isopropyl alcohol won't stop trichoderma. Good to know!
Edited by vanceen (01/17/23 01:12 PM)
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JHOVA
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Re: Flow hood - how long to run before use? [Re: vanceen] 1
#28144457 - 01/17/23 01:32 PM (1 year, 2 months ago) |
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Wait a minute why is your pf tek success so low? Even a dirty syringe should work most times.
Are you venting your pc? How long?
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vanceen
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Re: Flow hood - how long to run before use? [Re: JHOVA]
#28144482 - 01/17/23 02:00 PM (1 year, 2 months ago) |
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Quote:
JHOVA said: Wait a minute why is your pf tek success so low? Even a dirty syringe should work most times.
Are you venting your pc? How long?
My PF Tek jars were all purchased, pre-sterilized from Midwest.
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vanceen
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Re: Flow hood - how long to run before use? [Re: vanceen]
#28144494 - 01/17/23 02:07 PM (1 year, 2 months ago) |
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Log in to view attachment
I found a technical paper about the effectiveness of different disinfectants on trichoderma spores and mycellium.
According to this, bleach is good on spores but needs a 1:5 dilution to work fast.
The best on spores was a product called Disolite in the UK, which is a mixture of 2-phenyphenol, benzyl-chlorophenol and propan-2-ol (isopropyl alcohol).
Peracetic acid was not very effective.
[url=http://microbiotech.co.uk/Green_MouldA.pdf]
Edited by vanceen (01/17/23 02:11 PM)
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Thugnar
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Re: Flow hood - how long to run before use? [Re: vanceen]
#28144495 - 01/17/23 02:08 PM (1 year, 2 months ago) |
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Quote:
vanceen said:
Quote:
JHOVA said: Wait a minute why is your pf tek success so low? Even a dirty syringe should work most times.
Are you venting your pc? How long?
My PF Tek jars were all purchased, pre-sterilized from Midwest.
Is this really a thing? Im new to this site and i feel like i could make lots of money by sterilizing rye berries for my countrymen. I wouldnt do though that because its stupid... Sorry but you really should start doing things yourself. That way you have atleast some way of knowing where you went wrong when you grow trich. Buying sterilized grain from a vendor is like not using a condom after two nights of cocaine & ketamine. Pretty wild.
-------------------- Seems like 99% of people in shroomery mushcult use the Bucket Tek. I do not and for saying this they call me arrogant cause i dont have pictures of my own mushrooms here. The mycoforum of my country just got their proof of shroomerys hostility. I do like it here though becaus of the agar. The archives are valuable.
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Screwup
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Re: Flow hood - how long to run before use? [Re: Thugnar] 1
#28144499 - 01/17/23 02:10 PM (1 year, 2 months ago) |
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Dang youโve been growing for this long and didnโt know you could buy premade substrate and spawn? Almost like youโve never grown before.
How was the subway shift honey?
Edited by Screwup (01/17/23 02:10 PM)
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vanceen
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Re: Flow hood - how long to run before use? [Re: Thugnar]
#28144503 - 01/17/23 02:13 PM (1 year, 2 months ago) |
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Quote:
Thugnar said:
Quote:
vanceen said:
Quote:
JHOVA said: Wait a minute why is your pf tek success so low? Even a dirty syringe should work most times.
Are you venting your pc? How long?
My PF Tek jars were all purchased, pre-sterilized from Midwest.
Is this really a thing? Im new to this site and i feel like i could make lots of money by sterilizing rye berries for my countrymen. I wouldnt do though that because its stupid... Sorry but you really should start doing things yourself. That way you have atleast some way of knowing where you went wrong when you grow trich. Buying sterilized grain from a vendor is like not using a condom after two nights of cocaine & ketamine. Pretty wild.
I make all my substrates now, except for agar dishes. It has not solved my problems.
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