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OfflineVisigoth
enthusiast

Registered: 11/28/01
Posts: 481
Last seen: 11 years, 3 months
Innoculation with agar wedge
    #587773 - 03/24/02 12:41 AM (22 years, 2 months ago)

When innoculating with a wedge of colonized agar, it isn't the agar wedge that make colonization occur so fast in birdseed or grain jars, it's the fact that u can shake them and distribute the mycelium and the extra air exchange w. the polyfill or filter disc lids, is this correct? If so, how long (on average) would u expect a pint jar of BRF or Rye to colonize if kept at ideal temps and innoculated w. agar wedge?? thanks!!!

Vis


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"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

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OfflineVisigoth
enthusiast

Registered: 11/28/01
Posts: 481
Last seen: 11 years, 3 months
Re: Innoculation with agar wedge [Re: Visigoth]
    #588933 - 03/25/02 11:21 AM (22 years, 1 month ago)

Bump Bump!!!!


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"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 3 months, 9 days
Re: Innoculation with agar wedge [Re: Visigoth]
    #589033 - 03/25/02 02:07 PM (22 years, 1 month ago)

Part of why colonization is faster from agar is because you don't have to wait around for germination of the spores. My friend can get four cups of rye berries to fully colonize from agar transfer in under two weeks at 83F in total darkness. Four cups, under the same conditions, will fully colonize in under one week with the addition of one cup of spawn rather than an agar wedge.

No idea on timeframes for pint jars. I wouldn't use pint jars or BRF with agar transfer. The jars are too small to bother with and the BRF is sticky and hard to shake up.


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Just another spore in the wind.

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InvisibleNighted
Ghost

Registered: 11/09/01
Posts: 698
Loc: Funkytown
Re: Innoculation with agar wedge [Re: Visigoth]
    #589109 - 03/25/02 04:03 PM (22 years, 1 month ago)

No, it's because you are starting with live, healthy tissue rather than from dormant spores that must have time to germinate.


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InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Innoculation with agar wedge [Re: Nighted]
    #589591 - 03/26/02 12:52 AM (22 years, 1 month ago)

Nighted's right - and your also (hopefully) working with an isolated strain


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OfflineTieuChiCong69420
Stranger
Registered: 12/04/22
Posts: 1
Last seen: 1 year, 3 months
Re: inoculation with agar wedge [Re: Nighted]
    #28141207 - 01/15/23 12:47 PM (1 year, 4 months ago)

Saving for later hehe

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OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,340
Last seen: 2 months, 13 days
Re: inoculation with agar wedge [Re: TieuChiCong69420] * 1
    #28141220 - 01/15/23 12:59 PM (1 year, 4 months ago)

Trolling already huh?

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