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NoobShroob
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First time oat jars; inoculation questions.
#28137425 - 01/13/23 01:37 AM (2 years, 4 days ago) |
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First time doing oat jars theyre in the pc now. this thing gets loud, coin trick from bod and its getting up there, but its worth the process, hopefully. My pc doesnt have a pressure gate but if its loud with the coins im assuming im in safe territory(15 weight plus 2 quarters). Im curious what the minimum time is. Its been vented. Also of course agar would be the best method but what would be the best for a spore syringe? Just open the lid carefully and shoot it in and lock it back up? Or inject it into it with the lid off. If i were to put a hole in the lid i would need something very sterile which at that point i feel i should use the syringe and forget a hole, opinions?
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Edited by NoobShroob (01/13/23 01:52 AM)
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28137453 - 01/13/23 02:50 AM (2 years, 4 days ago) |
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Hour n a half later its off the stove maybe an hour 40, also 10 minutes of venting. Im just gonna let the pressure slowly release and take them out tomorrow, let it get all the time it can in lol
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DERRAYLD
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28137463 - 01/13/23 03:11 AM (2 years, 4 days ago) |
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Are you doing oat jars with unmodded lids? Are you keeping them loose or tightening them?
Edited by DERRAYLD (01/13/23 03:26 AM)
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: DERRAYLD]
#28137650 - 01/13/23 08:42 AM (2 years, 4 days ago) |
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They are loose with foil over top and unmodified
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ruawakeyet


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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 2
#28137663 - 01/13/23 08:52 AM (2 years, 4 days ago) |
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The real question is why are you shooting spores into grains? That's just really bad practice. Not having modded lids for that already bad practice only adds to it.
You should be using PF Tek if you want to inoculate using a spore syringe. You need to be using agar if you want to use any grain tek.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: ruawakeyet]
#28137673 - 01/13/23 09:00 AM (2 years, 4 days ago) |
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Im well aware i already said in my post that this is my available options at the moment, i know it isnt ideal but its what we got and will change asap. So anyways mind answering which way would be best? since i have to remove a lid regardless i can add the hole now and sanitize as i have the special tape it is possible for me to cover it up after. Or i can just shoot it on top, inject it, and/or leave the lid loose or of course add holes and inject through that ext.
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Edited by NoobShroob (01/13/23 09:02 AM)
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ruawakeyet


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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 2
#28137677 - 01/13/23 09:04 AM (2 years, 4 days ago) |
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Sorry, it's too late to add a hole to the lid. I'd give it a 99.9% chance it will contaminate. If you're going to shoot spores into grains, the best chance is to barely lift the lid in a SAB, and inject some drops of solution into it. The less solution you use, the better the chances of not getting contaminants.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: ruawakeyet] 1
#28137685 - 01/13/23 09:09 AM (2 years, 4 days ago) |
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Now this im sure is more bad practice, however ive already used this syringe and its worked beautifully so unless its go contam from sitting my my drawer i know the syringe is clean so ideally it should still be.
Im going to do two, maybe three just to be sure.
I will inject one using the method we talked about in a sab, im also gonna make a modified lid, sanitize the hell out of it, and swap it with another lid real quick so ideally nothing should have an effect.
Id like a control but i guess that isnt an option. To be honest my first time using a pressure cooker and it has no gauge so im praying the oats are even right😭
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Edited by NoobShroob (01/13/23 06:48 PM)
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ruawakeyet


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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 1
#28137693 - 01/13/23 09:17 AM (2 years, 4 days ago) |
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Are you using the 16 qt. Presto with the adjustable jiggle weight? (5/10/15 psi) I don't trust that thing beyond 15 psi. That's what I have, and the bottom is already pushed out to the point it's visibly convex shaped, and rocks on my flat-top range unless I shim it with a few razor blades. I've never added weight to the jiggle weight and it still has pushed out like that.
Keep us updated on how everything works, it could help others in the future.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: ruawakeyet] 1
#28137716 - 01/13/23 09:28 AM (2 years, 4 days ago) |
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I am, i used it for the first time yesterday but no signs of warping, yet lol.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 1
#28137743 - 01/13/23 10:03 AM (2 years, 4 days ago) |
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So the grain looked good, perfect humidity id say as far as i can tell. I went ahead and made 2-3 modified lids and 2-3 regular unmodified style. I have three i ran out of spore solution for so i guess they will sit to the side for now, tape covering the holes and now we sit and wait to see what, if anything, happens. Wish me luck. It was all id say a clean process, my idea should i have issues was that the pressure didnt get quite right more so than i think contam coming from the lid swap. If they all fail it would clearly be because of that, or bad spore syringe but i believe ive taken great care of that as well. Time will tell
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28137746 - 01/13/23 10:07 AM (2 years, 4 days ago) |
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Box of grain

Close up of one with the tape and holes.
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Kevinarns



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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 1
#28138338 - 01/13/23 05:25 PM (2 years, 3 days ago) |
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The lid swap is definitely contaminated already. If it wasn’t cooked along with the rest, it’s dirty. Sorry. 😢 since you have a pc, why not do agar? It’s super easy and it works wonders.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: Kevinarns]
#28138372 - 01/13/23 05:47 PM (2 years, 3 days ago) |
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What makes you so sure that it is contaminated, the lid can be sterilized otherwise the entire point of the pc is sterilize the oats?
The glass and lid can be sterilized with alcohol or a million things. Unless im missing something? Also again im in the process of getting to agar i used what i had as a trial run. I used 2$ worth of oats so if it is contam oh well im just curious how you can be sure its contam from simply changing the lid with another sterile lid. Hell you have to remove the lid to put the agar in, the timeframe its exposed is almost identical.
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RoscoeReturns
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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 2
#28138398 - 01/13/23 05:59 PM (2 years, 3 days ago) |
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Alcohol is a sanitizer, not a sterilant. Very different.
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ruawakeyet


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Re: First time oat jars; inoculation questions. [Re: Kevinarns] 2
#28138415 - 01/13/23 06:09 PM (2 years, 3 days ago) |
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Noob, did you wrap the lids in foil, PC them, and then swap them in the SAB? Also, did you grab the lids using the lid bands in place, or only the lids with your fingers on the edge of them, then installed the lid bands?
If you only doused them in ISO and assumed they were sterile, then they weren't.
The one-piece plastic lids are easier to work with in terms of keeping fingers away from the rim of the jars and the edges of the lids.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: ruawakeyet]
#28138480 - 01/13/23 06:44 PM (2 years, 3 days ago) |
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Gloves, iso, sab, rim only. Now maybe it didnt 100% sterilize but the iso had to do a decent amount i feel like its got a fighting chance unless yall are 1000% confident its fucked. theres always at least one saying somethings contam on a perfect setup you know, im not saying yall are wrong but ive seen people say contam and bad methods ext that still work fine, what are my chances i guess is the best question. Its a pretty aggressive strain. The lid was inside of the band and i flipped it on top to minimize contact.
Maybe not sterile but the grain is whats going to be inoculated and its sterile along with the glass, the only exception is the lid it isnt touching aaaand its maybe not sterile but super clean. I feel as if thats got to mean something or some sort of fighting chance.
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ruawakeyet


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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 1
#28138502 - 01/13/23 06:50 PM (2 years, 3 days ago) |
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As long as you don't shake the jar, there's a chance. It might be a slim chance, but nothing is impossible.
I had assumed you PCed the lids when you meant said you were going to sterilize them.
ISO only sanitizes, so just remember that in the future.
Edited by ruawakeyet (01/13/23 07:00 PM)
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: ruawakeyet]
#28138515 - 01/13/23 06:53 PM (2 years, 3 days ago) |
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Honestly i knew that i just got my words mixed up, also i did shake them to mix in the spores too late now lolol. Sucks to suck i guess all i can do at this point is pray, i will keep yall updated
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28144821 - 01/17/23 05:18 PM (1 year, 11 months ago) |
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4 days in and i see nothing going on in any of them, could need more time but i see no bacteria, mold, myc, nothing so does tthis mean anything?
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ruawakeyet


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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28144843 - 01/17/23 05:28 PM (1 year, 11 months ago) |
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No, not really. Cube spores typically take 5 to 7 days to germinate visibly. Bacteria on grain is pretty much invisible, other than when mycelium responds to it. Mold spores can germinate quickly, but I've also seen it take as long as 10 days on agar after opening a lid before visible mold germination.
Unfortunately, all you can really do on them is wait for now.
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BeefSupremeJr
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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 1
#28144917 - 01/17/23 06:02 PM (1 year, 11 months ago) |
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ten days is typical to see something... you can tell people dont like to see this technique but im praying for you bud
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: BeefSupremeJr]
#28152859 - 01/22/23 09:02 PM (1 year, 11 months ago) |
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I try not to check everyday but its hard not to
One of them is im 99% sure contam and is growing, the rest show no signs of growth of anything.
Imo this could mean maybe the others have a chance? or just wont grow at all.
I forget the name but it looks like cobweb and i know that a go to sign of a certain bacteria the jars have mineral deposits(vinegar next time for sureeee lol) but if you look close you can see the difference in depth.

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Edited by NoobShroob (01/22/23 11:01 PM)
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28157639 - 01/25/23 08:32 PM (1 year, 11 months ago) |
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Alright update update, day 12 and its not looking great. The three that were swapped out after sterilization for sanitized lids have three different things, one shoes no sign of growth, and one shows a cobweb mold, the other a different type of mold. Let me know what yall think they are. The two that had their lids flipped after sterilization have different things happening, one is empty with nothing, the other with possible mycelium growth.
Lets start on the possible high note. To me, this looks like mycelium growth, i dont like the fact it took 12 days but if yall say 10 is average then thats not to far off, the lids were sterilized just flipped after pc'd.


Maybe ill get a jar out after all?
Now on to the bad This is the one that was posted two days ago, ive moved it away from the rest and put it in its own box soon to be properly exposed of. There is a lot of mineral stain on the glass but you can see cob web inside.


 Best picture of this imo is this one, you can see the difference in glass mineral deposit and web inside.

On to the other swapped lid this new and different mold is growing here.
  
So im assuming out of the two i have with zero signs of growth, its safe to assume the swapped lid will soon become mold, and the other MAY grow mycelium? or possible have no growth at all. Now should mycelium i have in this one jar continue to grow, i have the lid on not super tight but relatively tight, just i have it loose or keep it tight or somewhere in between, keep in mind the inner metal inside the ring is upside down, i remember hearing that allows air exchange but i didnt remember if it did on its own or still needed to be lose or tight,
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Edited by NoobShroob (01/25/23 08:36 PM)
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28157671 - 01/25/23 09:06 PM (1 year, 11 months ago) |
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Also just to clarify im not positive any of the above is mold or mycelium, just speculation, im open to opinions and of course hard answers if possible.
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johnukguy
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28157803 - 01/26/23 12:55 AM (1 year, 11 months ago) |
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If you take pics that are more in focus, we can help you more easily. It's hard to tell what's going on otherwise. Some of the pics, to me, look like mycelial growth but can't be sure without more clarity and probably a few more days.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: johnukguy]
#28157924 - 01/26/23 06:36 AM (1 year, 11 months ago) |
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Its completely in focus, the glass in general, especially the curvature of it distorts the imagine similar to looking under water, into a fish tank ect, the only way i can get better pics is lid off, which for the mold probably could but id rather not open the myc until its stalled or fully colonized.
Everything looks good similar irl except the cobweb it hard to see on its own as its thin and cobwebby but im pretty sure thats a common mold, at the very least def not myc. The biggest issue id say is the mineral deposits making it hard too see but its too late to change that now lol
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28160534 - 01/27/23 09:22 PM (1 year, 11 months ago) |
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Two weeks and ive seen no growth in two jars, should i just dump them or leave them be? one of the lid swapped ones has visible moisture in it.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28163154 - 01/29/23 04:07 PM (1 year, 11 months ago) |
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So 3 days since my previous pictures and i believe 2 weeks or so after inoculation. Growth looks good and steady with no visible signs (that i know of at least) of contam. My only thing is that it kind of looks dry? should it be is there anything i can do? maybe try to g2g it or should i just let it rock as its on the right path so far.
 
 
also zero growth in the other 2 so far, im starting to think i should just dump them
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Edited by NoobShroob (01/29/23 04:14 PM)
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ruawakeyet

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Re: First time oat jars; inoculation questions. [Re: NoobShroob] 1
#28163162 - 01/29/23 04:12 PM (1 year, 11 months ago) |
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I would leave them be since they are looking good.
The jars that still aren't doing anything could be dumped. I would say it's been long enough to give up on them. You might smell them before you dump them as long as there is no mold visible. If it's bacterial, you can learn what that smells like and still get as much experience from the process as possible.
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: ruawakeyet] 1
#28163167 - 01/29/23 04:16 PM (1 year, 11 months ago) |
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Thanks. i was thinking the same but its always nice to hear more opinions than just base off what i assume
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28180038 - 02/09/23 07:04 PM (1 year, 11 months ago) |
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the final picture is from earlier today. Right one is the sketchier one and its looking the healthiest! Do yall think it looks good it has a little black dot but hasnt gotten bigger or changed over the past few days. I think its ready to goto spawn as well probably tomorrow opinions?
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BeefSupremeJr
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Re: First time oat jars; inoculation questions. [Re: NoobShroob]
#28180049 - 02/09/23 07:09 PM (1 year, 11 months ago) |
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nah. let em sit a minute. couple more days
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NoobShroob
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Re: First time oat jars; inoculation questions. [Re: BeefSupremeJr]
#28180065 - 02/09/23 07:27 PM (1 year, 11 months ago) |
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