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Offlineemetheus
enthusiast
Registered: 10/26/00
Posts: 149
Last seen: 15 years, 7 months
Re: 2 weeks into colonization - but nobody's home!
    #281364 - 03/29/01 11:50 AM (15 years, 8 months ago)

the heat prolly killed them.


Visit http://mycotopia.yage.net


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InvisibleG-nome
member
Registered: 08/18/00
Posts: 73
Re: 2 weeks into colonization - but nobody's home! [Re: emetheus]
    #281421 - 03/29/01 11:54 AM (15 years, 8 months ago)

(crying) That just fucking sucks, man. I don't know what to do.



--------------------
"Getting an inch of snow is like winning ten cents in the lottery." - Calvin
The Free Spore Ring - the best damn thing since sliced bread?


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Offlineemetheus
enthusiast
Registered: 10/26/00
Posts: 149
Last seen: 15 years, 7 months
Re: 2 weeks into colonization - but nobody's home! [Re: G-nome]
    #281434 - 03/29/01 12:16 PM (15 years, 8 months ago)

ryche's pretty cool about these sorts of things.
why don't you email him explaining your problem ?
he just might replace yours.

Visit http://mycotopia.yage.net


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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: 2 weeks into colonization - but nobody's home! [Re: emetheus]
    #281461 - 03/29/01 01:48 PM (15 years, 8 months ago)

Sorry, G-dude, sounds like you baked `em. That one might make it. Better order some more syringes. Don`t feel to bad, few of us got it right the first time.



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Offlinebluhoney
Yes Im a realgirl ,geeesh
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Registered: 05/24/99
Posts: 936
Last seen: 9 years, 9 months
Re: 2 weeks into colonization - but nobody's home! [Re: G-nome]
    #281496 - 03/29/01 02:41 PM (15 years, 8 months ago)

You say you have that one jar with growth already present. My suggestion would be to use a glove box of some sorts and very Cleanly, remove a piece and innoculate another jar, then take another piece and innoculate another, until you use all the mycelia or run out of jars. That will knock out some of the time required to start innoculation. A homemade wire loop will do fine for this task or a pair of tweezers. Either way, make sure to work in a VERY clean enviroment.

Its two in the morning......Do you know where your paperbag and flashlight are?


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InvisibleG-nome
member
Registered: 08/18/00
Posts: 73
Re: 2 weeks into colonization - but nobody's home! [Re: bluhoney]
    #281502 - 03/29/01 02:49 PM (15 years, 8 months ago)
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Is there a way to combine this with the honey water tek or some thing along that line?



--------------------
"Getting an inch of snow is like winning ten cents in the lottery." - Calvin
The Free Spore Ring - the best damn thing since sliced bread?


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Offlinebluhoney
Yes Im a realgirl ,geeesh
Female User Gallery

Registered: 05/24/99
Posts: 936
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Re: 2 weeks into colonization - but nobody's home! [Re: G-nome]
    #281523 - 03/29/01 03:15 PM (15 years, 8 months ago)

My friend has not attempted to introduce already present mycelia in honey water only spores. It should work hypothetically. And then you can use the honey water/mycelia to innoculate other jars.

Its two in the morning......Do you know where your paperbag and flashlight are?


--------------------
Information listed here is for entertainment only and is neither real or proven


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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: 2 weeks into colonization - but nobody's home! [Re: G-nome]
    #281533 - 03/29/01 03:22 PM (15 years, 8 months ago)

Yes there is, although honey water can be contaminate prone. As bluhoney has said, you`ve got to be clean.



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InvisibleG-nome
member
Registered: 08/18/00
Posts: 73
Re: 2 weeks into colonization - but nobody's home! [Re: bluhoney]
    #281610 - 03/29/01 04:14 PM (15 years, 8 months ago)

Exactly how did your friend do that?



--------------------
"Getting an inch of snow is like winning ten cents in the lottery." - Calvin
The Free Spore Ring - the best damn thing since sliced bread?


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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: 2 weeks into colonization - but nobody's home! [Re: G-nome]
    #281671 - 03/29/01 05:26 PM (15 years, 8 months ago)

OK, 1 oz. honey per quart of water. Honey water goes into jar and is steamed for 1 hour. Let cool, with sterile knife scoop out a chunk of mycelia. Give mycelia a 30 second bath in pure hydrogen peroxide. Immediately transfer mycelia to jar of honey water. Shake the living shit out of it. After two days inject 1cc H2O2. At room temp. in 3 - 4 days honey water should be cloudy with mycelia. Good sterile practice and common sense make the difference between success and failure.
Glove box, flow hood, oven tek, good. Fingers, jar sitting around with lid off, too sweet honey water, bad.



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