Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Mono Tub Substrate   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
OfflineHindsight
Mad Scientist
 User Gallery

Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 3 days
Trusted Cultivator
Round objects inside mycelium when viewed under microscope * 3
    #28132117 - 01/09/23 12:00 PM (1 year, 18 days ago)

Was looking at a drop of colonized LC culture under a microscope today and I notice a lot of little round spots. They appear inside the confines of the mycelium and many of them are moving around. They do not look like bacteria to me so it isn't that, and they appear to be inside the walls of the mycelium. Does anyone know what these are?



Extras: Filter Print Post Top
Offlinefather_fungi
Mold Farmer
 User Gallery


Registered: 12/13/21
Posts: 245
Last seen: 1 month, 14 days
Re: Round objects inside mycelium when viewed under microscope [Re: Hindsight] * 1
    #28132201 - 01/09/23 01:09 PM (1 year, 18 days ago)

The little rounds spots are likely organelles of some type, probably nuclei.  Mycelia cells have septum where they connect to each other and cytoplasm, along with organelles, can flow between them.  That's likely what you're seeing.

Thanks for sharing!  What species are we looking at? Have you found any clamp connections yet?


--------------------
Agar and Liquid Culture Formulas


Equipment, Teks, Etc
FF Lid Tek


Edited by father_fungi (01/09/23 01:12 PM)


Extras: Filter Print Post Top
OfflineHindsight
Mad Scientist
 User Gallery

Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 3 days
Trusted Cultivator
Re: Round objects inside mycelium when viewed under microscope [Re: father_fungi] * 1
    #28132258 - 01/09/23 01:45 PM (1 year, 18 days ago)

Thanks for the reply! That makes sense.

Yes, I think I have seen clamp connections. Photo was taken at 1000x. It's a bit tough to ensure I'm seeing clamp connections but I'm pretty sure that is what they are based on comparing what I've seen online with what I'm seeing through my scope.

Species was either Cubensis or Subtropicalis as those were the two LC cultures I was looking at today but I forget which one I took this photo of. I've been using the scope to check for the presence of bacteria in my LCs - I have found that not all bacteria will make your LC go cloudy so using clarity alone is not a definitive test for bacterial contamination.


Extras: Filter Print Post Top
OfflineHindsight
Mad Scientist
 User Gallery

Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 3 days
Trusted Cultivator
Re: Round objects inside mycelium when viewed under microscope [Re: Hindsight] * 2
    #28133647 - 01/10/23 12:49 PM (1 year, 17 days ago)

Some clamp connections:





Extras: Filter Print Post Top
OfflineTwistedzombie66
Stoner
Male User Gallery

Registered: 10/17/21
Posts: 406
Last seen: 2 months, 10 days
Re: Round objects inside mycelium when viewed under microscope [Re: Hindsight]
    #28136212 - 01/12/23 04:48 AM (1 year, 15 days ago)

That's so cool!!

Twisted 🤘🤘


--------------------






Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Mono Tub Substrate   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Contam eating mycelium?
( 1 2 all )
Anonymous 4,990 35 08/06/03 09:41 AM
by micro
* Honey mycelium... wikedanjel 2,923 8 07/30/02 09:05 AM
by CaliChronic
* Microscopes EffedS 2,313 15 12/20/03 02:07 PM
by Effed
* Microscope recommendations anyone? armedia 2,456 6 04/24/02 05:20 AM
by zeronio
* recommend a microscope? Olgualion 1,017 4 02/23/03 09:52 PM
by zeronio
* Mycelium Culture Erik006 1,397 7 04/01/04 10:39 AM
by Anonymous
* AHH! possible mono-nuclear mycelium cloned! growin 1,651 5 01/10/03 02:45 PM
by deanofmean
* MIcroscope use! PooPs 1,644 8 05/16/03 03:44 PM
by micro

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
485 topic views. 1 members, 3 guests and 4 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.004 seconds on 12 queries.