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OnlineBeefSupremeJr
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Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr]
    #28135888 - 01/11/23 07:41 PM (2 years, 5 days ago)

so prestos with jiggleweights vent themselves.  So the question is, OP, what kind of pc you using?

jiggle or toggle?

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 1
    #28135901 - 01/11/23 07:51 PM (2 years, 5 days ago)

Mine has a jiggle weight and pressure gauge. I usually vent about 15 mins.

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OnlineBeefSupremeJr
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Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName] * 1
    #28135914 - 01/11/23 07:57 PM (2 years, 5 days ago)

lid pics?

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OfflineFlawdaKraka
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Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName] * 1
    #28135918 - 01/11/23 07:59 PM (2 years, 5 days ago)

Check your lids for damage, check/replace the poly as needed.

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InvisibleLucifer944
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Re: Unused grain jars contam, but agar ok? [Re: FlawdaKraka] * 3
    #28135929 - 01/11/23 08:05 PM (2 years, 5 days ago)

Venting is definitely a thing. Yes, vented pressure cookers will naturally vent themselves but you wouldn't really know when exactly it has been fully vented. You could technically under sterilize in this case if you followed typical times.

If the jars were never opened after pressure cooking I would say you either aren't getting full sterilization or something is screwy with your lids.

What elevation are you at?

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: Lucifer944] * 1
    #28135983 - 01/11/23 08:39 PM (2 years, 5 days ago)

I believe I am at high elevation. Lids are just metal or plastics stuffed tight with polyfil.

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OnlineBeefSupremeJr
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Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName]
    #28135991 - 01/11/23 08:45 PM (2 years, 5 days ago)

im not sure i can help you anymore here but i feel like wbs seems to contam more than other grains.  i like wheat

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OfflineFlawdaKraka
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Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName] * 1
    #28135999 - 01/11/23 08:51 PM (2 years, 5 days ago)

Look around for time adjustments for higher elevations, idk what they are, but you'll need to increase times from what they are at sea level.

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: FlawdaKraka]
    #28136005 - 01/11/23 08:53 PM (2 years, 5 days ago)

Im goin to give the no cook prep a go and see what happens. My wbs prep before going in the pc seems to be the odd ball out in comparison to the technique of others.

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OfflineClusterB
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Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName] * 1
    #28136157 - 01/12/23 12:56 AM (2 years, 5 days ago)

I've been using Hamloaf's WBS prep and it works really well for me so far.
https://www.shroomery.org/forums/showflat.php/Number/24952939/fpart/1/vc/1

My last set of WBS jars, I Used FooMans prep method.
https://www.shroomery.org/forums/showflat.php/Number/8508523

The jars made it to their shoeboxes and are now colonizing. They looked great when finished but were slower. I'm convinced it was due to cooler temps, or could have been on the drier side of ideal. Maybe it was due to the genetics of what I am running?

I'm favoring the Hamloaf WBS tek, but as a noob, I'm just happy to be getting shit to the finish line.


--------------------
I used to do drugs. I still do, but I used to too.
-Mitch Hedberg

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: ClusterB]
    #28136280 - 01/12/23 07:00 AM (2 years, 5 days ago)

Quote:

ClusterB said:
I've been using Hamloaf's WBS prep and it works really well for me so far.
https://www.shroomery.org/forums/showflat.php/Number/24952939/fpart/1/vc/1

My last set of WBS jars, I Used FooMans prep method.
https://www.shroomery.org/forums/showflat.php/Number/8508523

The jars made it to their shoeboxes and are now colonizing. They looked great when finished but were slower. I'm convinced it was due to cooler temps, or could have been on the drier side of ideal. Maybe it was due to the genetics of what I am running?

I'm favoring the Hamloaf WBS tek, but as a noob, I'm just happy to be getting shit to the finish line.




Yeah Im following Hamloaf’s tek right now, but im considering trying a method without the boil such as SpitballJedi where it soaks 12-24 hours then gets drained before loading up the jars.

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 1
    #28136363 - 01/12/23 08:42 AM (2 years, 5 days ago)

Quote:

BeefSupremeJr said:
lid pics?



Lids are the same on my wbs as they are on my agar jars. I have zero problems with contams on agar.


Edited by FunnyFungiName (01/12/23 08:42 AM)

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OfflineFlawdaKraka
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Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName] * 2
    #28136421 - 01/12/23 09:37 AM (2 years, 5 days ago)

Here's a chart from AA for pressure to sterilize at elevation, you never did say what your elevation is. But these are the PSI to reach sterilization at normal times.



If you can't reach this pressure for your elevation, you'll need to increase time. Here's a good link for info.

PCing at altitude

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Offlinedonrjuan
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Re: Unused grain jars contam, but agar ok? [Re: FlawdaKraka] * 2
    #28136440 - 01/12/23 09:57 AM (2 years, 5 days ago)

That link is a fantastic resource. At nearly 6K' I have always just increased time since all I have are prestos

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: FlawdaKraka] * 2
    #28136522 - 01/12/23 11:11 AM (2 years, 5 days ago)

Quote:

FlawdaKraka said:
Here's a chart from AA for pressure to sterilize at elevation, you never did say what your elevation is. But these are the PSI to reach sterilization at normal times.



If you can't reach this pressure for your elevation, you'll need to increase time. Here's a good link for info.

PCing at altitude




Thank you that is very useful. I am at about 1800 above sea level, but I am usually over 15psi for most of a cycle.

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OnlineB Traven
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Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 1
    #28136567 - 01/12/23 11:56 AM (2 years, 5 days ago)

Quote:

BeefSupremeJr said:
so prestos with jiggleweights vent themselves.




Not sure I'm following you there.

This is the removable weight, right?

You gotta take it off until a steady flow of steam emerges from the stem, which seems like the opposite of self-venting to me.

The extra-sure version is just letting that process go on for 15 minutes before putting the weight on. I find that it doesn't even add any extra time to the cycle.


--------------------
Beware of advice- even this.

Edited by B Traven (01/12/23 11:58 AM)

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InvisibleLucifer944
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Re: Unused grain jars contam, but agar ok? [Re: B Traven] * 1
    #28136584 - 01/12/23 12:11 PM (2 years, 5 days ago)

Quote:

B Traven said:
Quote:

BeefSupremeJr said:
so prestos with jiggleweights vent themselves.




Not sure I'm following you there.

This is the removable weight, right?

You gotta take it off until a steady flow of steam emerges from the stem, which seems like the opposite of self-venting to me.

The extra-sure version is just letting that process go on for 15 minutes before putting the weight on. I find that it doesn't even add any extra time to the cycle.




You're correct, you want leave it off and let it fully vent. Who knows how long it would take to actually self vent, could take hours in which case you wouldn't achieve the highest temperature.

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OnlineB Traven
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Re: Unused grain jars contam, but agar ok? [Re: B Traven] * 1
    #28136586 - 01/12/23 12:13 PM (2 years, 5 days ago)

I vote for the grain not being fully sterilized, as well.

If it were an issue with the lids, I'd also expect the contamination to be clustered at the top of the grain. And take on a specific and obvious character, like for instance little tufts of green trich right out the starting gate.

I also subscribe to the belief that no grain is truly fully sterile.

I try to use my jars as soon as possible after PC'ing, ideally the very next day. I think there are a number of cases where some spores in the jar might germinate and take over if given enough time, but get nowhere once the myc has taken hold.

My contamination rates definitely dropped a bit once I stopped letting jars sit around before inoculation.


--------------------
Beware of advice- even this.

Edited by B Traven (01/12/23 12:14 PM)

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: B Traven] * 1
    #28136596 - 01/12/23 12:25 PM (2 years, 5 days ago)

Quote:

B Traven said:
I vote for the grain not being fully sterilized, as well.

If it were an issue with the lids, I'd also expect the contamination to be clustered at the top of the grain. And take on a specific and obvious character, like for instance little tufts of green trich right out the starting gate.

I also subscribe to the belief that no grain is truly fully sterile.

I try to use my jars as soon as possible after PC'ing, ideally the very next day. I think there are a number of cases where some spores in the jar might germinate and take over if given enough time, but get nowhere once the myc has taken hold.

My contamination rates definitely dropped a bit once I stopped letting jars sit around before inoculation.




Im almost positive that the jars sitting too long is my issue.

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OfflineFunnyFungiName
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Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName]
    #28187042 - 02/14/23 11:45 AM (1 year, 10 months ago)

Quote:

FunnyFungiName said:
I bring my wbs to a boil, simmer 5 mins, drain for 45, pc 2 hours at 15 psi.




After reading a ton of wbs teks and oat teks it seems maybe my wbs jars were getting contams because they weren’t hydrated well enough.

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