Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
OfflineFunnyFungiName
Ninja Space Pirate
Male User Gallery

Registered: 01/15/18
Posts: 342
Last seen: 15 minutes, 59 seconds
Unused grain jars contam, but agar ok?
    #28135656 - 01/11/23 05:10 PM (2 years, 5 days ago)

In order to track down the source of my spawn comtams I left sitting for a month 6 jars of wbs quarts and 15 no pour agars in 1/2 pint mason jars. All wide mouth, some plastic, some metal, all polyfil filters.

After one month all agar still look good as new, but 3 of my wbs jars have contams. 2 have a dirty grainy residue that the wbs leaves on the glass, one has a black mold.

Any idea why some of my wbs jars are going bad while agar and LCs in the same jars/lids/filters are fine?

Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName]
    #28135662 - 01/11/23 05:16 PM (2 years, 5 days ago)

it isnt the jar.  id bet my life savings on that.  its the wbs.  bodhi always said theres no such thing as sterile grain and as time goes on, i realize the wisdom of that grouchy sonofabitch.

Extras: Filter Print Post Top
OfflineFunnyFungiName
Ninja Space Pirate
Male User Gallery

Registered: 01/15/18
Posts: 342
Last seen: 15 minutes, 59 seconds
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr]
    #28135680 - 01/11/23 05:31 PM (2 years, 5 days ago)

Quote:

BeefSupremeJr said:
it isnt the jar.  id bet my life savings on that.  its the wbs.  bodhi always said theres no such thing as sterile grain and as time goes on, i realize the wisdom of that grouchy sonofabitch.




I usually make my grains days or a week before I use them so maybe that is the issue.

Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName]
    #28135684 - 01/11/23 05:34 PM (2 years, 5 days ago)

probably not.  ive had jars sit around for a weeks.
whats your prep procedure?  how long is your pc cycle? what pressure?

Extras: Filter Print Post Top
OfflineFunnyFungiName
Ninja Space Pirate
Male User Gallery

Registered: 01/15/18
Posts: 342
Last seen: 15 minutes, 59 seconds
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr]
    #28135699 - 01/11/23 05:48 PM (2 years, 5 days ago)

I bring my wbs to a boil, simmer 5 mins, drain for 45, pc 2 hours at 15 psi.

Edited by FunnyFungiName (01/11/23 05:48 PM)

Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName] * 1
    #28135707 - 01/11/23 05:51 PM (2 years, 5 days ago)

hmm.  i dunno.  i dont use wbs but id get in the habit of using it pretty readily once its prepped.  a month is way too long.  as ive said theres no such thing as sterile grain so its a foot race between our culture and the dormant endospores latent in the grain after its pc.

thats also why spores to grain is invariably a recipe for contam..

Extras: Filter Print Post Top
InvisibleSoutherner
Stranger

Registered: 03/25/21
Posts: 611
Loc: South
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 2
    #28135745 - 01/11/23 06:13 PM (2 years, 5 days ago)

Mold wouldn’t survive the pc cycle. You may need to inspect your lids/filters and make sure everything is properly done and you’re getting a seal with your lids. May even try wrapping around them a couple times with cling or something to help block out contaminants.

Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E Flag
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: Southerner]
    #28135755 - 01/11/23 06:19 PM (2 years, 5 days ago)

mold *spores* could totally survive a pc cycle in grain.  it makes sense that you wouldnt see it do anything for a month.

whats your lid situation?

Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery

Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr]
    #28135783 - 01/11/23 06:44 PM (2 years, 5 days ago)

Try prepping it without the boil for 5 minutes.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
InvisibleCreonAntigone
Stranger

Registered: 05/30/21
Posts: 3,374
Re: Unused grain jars contam, but agar ok? [Re: JHOVA] * 2
    #28135800 - 01/11/23 06:53 PM (2 years, 5 days ago)

Agar and LC are simply easier for the heat to penetrate. That is why grains are cooked at 2 hours typically while agar and LC one can even get away with 15-30 minutes.

Extras: Filter Print Post Top
OfflineFlawdaKraka
FNG
 User Gallery


Registered: 08/16/22
Posts: 588
Loc: Gulf Coast
Last seen: 1 month, 4 days
Re: Unused grain jars contam, but agar ok? [Re: FunnyFungiName] * 1
    #28135803 - 01/11/23 06:55 PM (2 years, 5 days ago)

Do you vent your PC? I was losing most of my jars to mold weeks after prep until I started venting for 15-20 mins.

Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E Flag
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: FlawdaKraka]
    #28135812 - 01/11/23 07:02 PM (2 years, 5 days ago)

do what? venting?

Extras: Filter Print Post Top
OfflineFlawdaKraka
FNG
 User Gallery


Registered: 08/16/22
Posts: 588
Loc: Gulf Coast
Last seen: 1 month, 4 days
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 2
    #28135839 - 01/11/23 07:17 PM (2 years, 5 days ago)

It's the only thing that changed in my prep. 15-20 mins of venting before bringing it up to pressure.

Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E Flag
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: FlawdaKraka]
    #28135844 - 01/11/23 07:19 PM (2 years, 5 days ago)

with all due respect, that isnt a thing

Extras: Filter Print Post Top
InvisibleMojo
Stranger

Registered: 07/12/07
Posts: 1,689
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 1
    #28135847 - 01/11/23 07:21 PM (2 years, 5 days ago)

I had a forgotten wbs grain jar sit for about 6 months. No contams. The grain had begun drying out though.

The jar had a plain metal lid and a single hole with poly-fil filter.

I don’t think it’s the prep.  Either the sterilization is bad, the handling is bad, the filter is bad, or the seal on the jar is bad.

What is the filter material?
What kind of lids are you using?
Are you running injection ports? Are they lose or deteriorating?

Does your pc have a gauge or are you assuming 15psi?
What is the elevation?

Extras: Filter Print Post Top
OfflineFlawdaKraka
FNG
 User Gallery


Registered: 08/16/22
Posts: 588
Loc: Gulf Coast
Last seen: 1 month, 4 days
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 3
    #28135848 - 01/11/23 07:22 PM (2 years, 5 days ago)


Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: Mojo]
    #28135860 - 01/11/23 07:28 PM (2 years, 5 days ago)

Same.  I have had jars of wheat sit for months without contaminating, but as a rule, I never assume grains to be sterile. 

Anyway, I concur.  There's something wrong with either the hardware or the process.  And pictures would really help.

Extras: Filter Print Post Top
OfflineFunnyFungiName
Ninja Space Pirate
Male User Gallery

Registered: 01/15/18
Posts: 342
Last seen: 15 minutes, 59 seconds
Re: Unused grain jars contam, but agar ok? [Re: JHOVA] * 1
    #28135865 - 01/11/23 07:29 PM (2 years, 5 days ago)

Lids are a mix some plastic some metal.
Quote:

JHOVA said:
Try prepping it without the boil for 5 minutes.




Im going to stop the boiling and try a 12-24 hour soak.

Extras: Filter Print Post Top
InvisibleMojo
Stranger

Registered: 07/12/07
Posts: 1,689
Re: Unused grain jars contam, but agar ok? [Re: BeefSupremeJr] * 2
    #28135866 - 01/11/23 07:29 PM (2 years, 5 days ago)

Quote:

BeefSupremeJr said:
with all due respect, that isnt a thing




It is a thing. The USDA recommends it for all canning and it is in the instruction manual for All American sterilizers.

Many vintage canners were sealed similar to AA sterilizers, so perhaps there was a case to be made for displacing the “dry” air trapped inside the canner with steam. 

These days no canners on the market are sealed and all of them vent to maintain pressure so it hardly seems necessary if you are running a shake weight…

Extras: Filter Print Post Top
OnlineBeefSupremeJr
Detritivore
Male User Gallery


Registered: 11/02/11
Posts: 8,680
Loc: 29.9792° N, 31.1342° E
Last seen: 4 seconds
Trusted Cultivator
Re: Unused grain jars contam, but agar ok? [Re: FlawdaKraka] * 3
    #28135868 - 01/11/23 07:31 PM (2 years, 5 days ago)

Quote:

FlawdaKraka said:
OK

The Importance of Venting a Pressure Cooker or Autoclave




huh! well, I'll be damned.  I will have to read this. I have never done this. I stand corrected, sir..
I've never seen it in the instruction manual for my unit but maybe I better read it again

Edited by BeefSupremeJr (01/11/23 07:32 PM)

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* When to case grain jars shianggu 3,414 1 04/30/02 07:23 AM
by humplok4201
* whole grain jars last?.................... Shadowfoot 1,536 1 10/29/02 04:17 PM
by SixTango
* Foamy yellow liquid in Rye Grain jar Visigoth 3,344 3 10/14/02 08:45 AM
by MAIA
* Noob here - myc or contam? (agar + grain) FryAndBones 279 6 03/13/23 02:15 PM
by Way
* Noob Here - Suspicious of Grain Jar, should I case in jar? BigSquid 342 8 02/13/23 11:36 AM
by BigSquid
* PF jar contams twice (now 3) in a row. upupup 5,945 18 05/30/19 02:12 PM
by Yowtzie
* About the lids on grain jars when sterilizing gonnadoit 2,793 7 09/29/02 05:09 PM
by mickey_rourke
* Re: inocculating old but unused sealed jars 3Mshroom 671 2 04/11/00 09:46 AM
by oOjonahOo

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
673 topic views. 53 members, 182 guests and 46 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.009 seconds on 14 queries.