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InvisibleOlgualion
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Agar technique question?
    #2811566 - 06/20/04 07:04 PM (12 years, 5 months ago)

When using peroxidated agar, it has been concluded that seemingly pure/clean cultures are harboring nasties. Transfers to grain sometimes turn green. Transfers to agar sometimes do the same. Given the amount of time it takes for the mold to envelop the plate, I would surmise that the contaminant must be from spores.

With a quick dip into a shotglass of diluted H2O2 to kill the mold spores, a previously infected wedge could probably now be assumed clean, right?

If so, how many dips could the shotglass of H2O2 take before it's desirable effects are lost?

Any thoughts whatsoever on this... contam'd h2o2 plates.... H2O2 in grain jars...


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InvisibleATWAR
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Re: Agar technique question? [Re: Olgualion]
    #2812148 - 06/20/04 11:19 PM (12 years, 5 months ago)

I dip all my transfers, regardless of source or media. Everything gets dipped into peroxide solution before it hits grain or agar. Sometimes it really isn't necessary for me to do so, but I do it anyway just in case.

Quote:

If so, how many dips could the shotglass of H2O2 take before it's desirable effects are lost?




You should change the peroxide solution between each transfer, for safety's sake. Peroxide is not effective againsed all bacteria, nor will it help with living mold mycelia. If you do not change the solution, then a contaminated wedge can cross-contaminate all subsequent transfers. It is possible for a H2O2 plate to easily become contaminated with the above mentioned sources just as a regular plate would. And of course it is possible for a fully colonized plate to harbor contaminants, despite peroxide in the mix...

Minimize exposure, maximize precautionary measures...


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InvisibleOlgualion
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Re: Agar technique question? [Re: ATWAR]
    #2813415 - 06/21/04 10:31 AM (12 years, 5 months ago)

Thanks for the reply, it helps...


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Invisiblepsyphon
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Re: Agar technique question? [Re: ATWAR]
    #2831684 - 06/26/04 10:03 PM (12 years, 5 months ago)

What concentration of peroxide do you use for dipping?

Thanks


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InvisibleATWAR
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Re: Agar technique question? [Re: psyphon]
    #2831954 - 06/27/04 12:24 AM (12 years, 5 months ago)

5-10% Hydrogen Peroxide to 90-95% Distilled and sterilized water.


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OfflineMike Elium
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Re: Agar technique question? [Re: ATWAR]
    #2834826 - 06/27/04 11:51 PM (12 years, 5 months ago)

Atwar,

Could you further clarify your last statement......is that taking standard 3%H2O2 and diluting it 95 to 5 (or 90 to 10)..... or truly a 5% (or 10%) H2O2 solution ? Thanks


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InvisibleATWAR
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Re: Agar technique question? [Re: Mike Elium]
    #2834871 - 06/28/04 12:11 AM (12 years, 5 months ago)

5-10% Pharmaceutical grade 3% Hydrogen Peroxide to 90-95% Distilled and sterilized water or 5-10ml 3% H2O2 to 90-95ml water...

I have accidentally used full strength peroxide with cubensis, and it pulled through.


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