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Invisiblemushroomexplorer

Registered: 04/05/10
Posts: 358
Loc: WA
Microscope stains
    #15892344 - 03/02/12 08:45 PM (11 years, 10 months ago)

I was wondering what stains people here like the best. Thanks for any input.


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InvisibleThe Lightning
Mycology Enthusiast
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Registered: 09/06/11
Posts: 3,889
Re: Microscope stains [Re: mushroomexplorer]
    #15893437 - 03/03/12 02:45 AM (11 years, 10 months ago)

1 drop of congo red with a few drops of 5% koh


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OfflineAmanita virosa
botanist by day
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Registered: 12/04/11
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Re: Microscope stains [Re: mushroomexplorer]
    #15893657 - 03/03/12 06:57 AM (11 years, 10 months ago)

Myc or spores?  if your staining mycelium, lactophenol blue works great.  Lugol's (iodine) also works for both spores and myc.


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Invisiblemushroomexplorer

Registered: 04/05/10
Posts: 358
Loc: WA
Re: Microscope stains [Re: Amanita virosa]
    #15893846 - 03/03/12 08:53 AM (11 years, 10 months ago)

For examining cystidia, I find I do not need stains for examining spores they are very visible regardless.


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OfflineAlan RockefellerM
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Re: Microscope stains [Re: mushroomexplorer]
    #15906433 - 03/05/12 09:56 PM (11 years, 10 months ago)

I have always wondered how well food coloring would work.


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InvisibleinskiM
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Re: Microscope stains [Re: Alan Rockefeller]
    #15906533 - 03/05/12 10:17 PM (11 years, 10 months ago)

I've tried it and as far as I can remember it was not so useful because the pigment was not absorbed by the cell walls, also, it needs to be diluted quite a bit as the pigment is extremely concentrated, it may be useful for some applications!


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InvisibleRigVedaLXVII.Soma
Bacteri/Phyc/Bry/Myc/ology
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Registered: 11/21/11
Posts: 380
Loc: Michigan
Re: Microscope stains [Re: inski]
    #15907589 - 03/06/12 04:45 AM (11 years, 10 months ago)

Quote:

Alan Rockefeller said:
I have always wondered how well food coloring would work.



Quote:

inski said:
I've tried it and as far as I can remember it was not so useful because the pigment was not absorbed by the cell walls, also, it needs to be diluted quite a bit as the pigment is extremely concentrated, it may be useful for some applications!





Yeah I remembering you mentioning this not too long ago Allan, in gsharpnolack's Microscopy notes thread, so I tried it and, forgot to report back the results.

One suggested stain I have seen for using Food Coloring was  using 1 drop of vinegar, 1 drop of alcohol, and 2 drops of red food colors, and 2 drops of blue coloring. (Or could just use one or the other, the reason for using both, gives more contrast.)

So I tried testing it on a Psilocybe cubensis gill section. It was very poor at absorbing into the specimen. It seemed to absorb into the middle more than the edges. So it refused to actually stain any of the basidia, or cystidia. Only very very lightly staining some, and some not stained at all.

But when it sat for maybe 20-30 minutes it stained it the inverted color for some reason. It would go on blue, and after sitting, would be pink. (and opposite).

I dont know. It might actually work if we could figure out a way to get the stain to absorb better. Or maybe add no alcohol, or no vinegar, etc. I am sure it could probably work, just needs some DIY tweaking.


But one thing that recipe is actually good for, is a Bacterial stain. Thats where I heard of it a long time ago. It works wonders at staining rod shaped and coccoid bacteria without staining its surroundings.


--------------------
"While clinging to the moronic belief that they constitute a "counterculture," they share our society's overriding urge for expediency. They make no attempt to learn about the organisms they eat and it always struck me as ironic that people with such a low level of consciousness should be seeking "higher consciousness." -David Arora

"Man is placed in the middle between two infinities - the infinitely great and the infinitely little - both of which are equally incomprehensible to him." -Pascal

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:peace: Solanaceae, Amanitaceae :peace:


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Invisiblehungrygoldfish
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Registered: 10/23/10
Posts: 1,165
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Re: Microscope stains [Re: Alan Rockefeller]
    #15907699 - 03/06/12 06:01 AM (11 years, 10 months ago)

Quote:

Alan Rockefeller said:
I have always wondered how well food coloring would work.




I tested some yellow food colouring last night and it showed up quite interesting but my microscope cut out due to a lose wire, I'm going to try and fix it and post some pictures later.

Can people try and post links to the stains that they buy so I can get an idea of what I should be looking out for.


--------------------
Come and visit my Photography Forum on the Shroomery!


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InvisibleRigVedaLXVII.Soma
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Registered: 11/21/11
Posts: 380
Loc: Michigan
Re: Microscope stains [Re: hungrygoldfish]
    #15914095 - 03/07/12 01:48 PM (11 years, 10 months ago)

Here is a pretty easily accessible, simple stain anyone can get.

Its called Malachite Green.

Its sold at any pet store, or ANY store that has a Fish section.
I actually just picked up a bottle at Miejer.

It is sold as a Ick and Protozoa Parasite Cure.
It was called "Quick Cure" under the brand name AP (Aquarium Products)

Ingredients: Formalin, Malachite Green.


Only cost 2.59, give it a try!


--------------------
"While clinging to the moronic belief that they constitute a "counterculture," they share our society's overriding urge for expediency. They make no attempt to learn about the organisms they eat and it always struck me as ironic that people with such a low level of consciousness should be seeking "higher consciousness." -David Arora

"Man is placed in the middle between two infinities - the infinitely great and the infinitely little - both of which are equally incomprehensible to him." -Pascal

MyPostImage Gallery  Trade List
Spores I Am Looking For  Seeds I am Looking For
:peace: Solanaceae, Amanitaceae :peace:


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InvisibleJavadog
Continuing along
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Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
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Re: Microscope stains [Re: RigVedaLXVII.Soma]
    #15915853 - 03/07/12 07:29 PM (11 years, 10 months ago)

Good stuff.  Thanks!


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OfflineRaptor333
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Registered: 12/20/22
Posts: 16
Last seen: 11 months, 3 days
Re: Microscope stains [Re: inski]
    #28108767 - 12/22/22 01:40 PM (1 year, 1 month ago)



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OfflineRaptor333
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Registered: 12/20/22
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Re: Microscope stains [Re: inski]
    #28109254 - 12/22/22 08:28 PM (1 year, 1 month ago)

Quote:

inski said:
I've tried it and as far as I can remember it was not so useful because the pigment was not absorbed by the cell walls, also, it needs to be diluted quite a bit as the pigment is extremely concentrated, it may be useful for some applications!




what strain is the first image of the set?


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OfflineAlan RockefellerM
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Registered: 03/10/07
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Re: Microscope stains [Re: Raptor333]
    #28112906 - 12/26/22 01:40 PM (1 year, 1 month ago)

Quote:

Raptor333 said:
Heita,

what strain is this?

https://www.shroomery.org/forums/thumbs/13-20/860559893-thumb_DSC_1250.jpg




Might be Psilocybe makarorae.


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