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Offlinerumfor69
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Enmeshed Bacteria?
    #28107382 - 12/21/22 01:24 PM (1 year, 1 month ago)

Is it possible for bacteria to be enmeshed in mycelium and move along with it across the agar?

I know water agar is a tool to use to get mycelium away from bacteria and works well for many people.

Hypothetical: What if you had no pour dishes that barely slowly or even not at all grew bacteria even when spit swabbed? Because condensation in the no pour 1/2 pint jar setup collected across the surface, evaporating and recollecting on the surface while in the PC cycle, ever becoming less and less nutritious but then solidifies that way once cool. Creating a paper thin layer of WA on the surface with nutrients right below it the mycelium can dig into...

In this Hypothetical scenario could bacteria that fell in or was present in a transferred piece then some how be moved and enmeshed along with the mycelium across the dish as the mycelium digs in for nutrients and grows like normal but the dish not providing ample surface nutrients for the bacteria to multiply enough to show its face or the mycelium to react enough to it to become noticeable?

Hopefully you understand my question, thank you


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OfflineLand TroutM
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Re: Enmeshed Bacteria? [Re: rumfor69]
    #28107442 - 12/21/22 02:14 PM (1 year, 1 month ago)

I’m no microbiologist.  Ive taken a look at a few of my own cultures under the scope and found bacteria of all kinds, some motile and whatever the opposite of motile is, types that sit there and types that move.  And these cultures will still colonize a bag of grain and put out canopies of fruits and quarter pounds on first flushes.  My theory is these bacteria have different diets than the types that cause problems for us, and why grain based nutrients work from agar to spawn.  I really don’t know for sure though.  All I know is I’ve seen them, and I see how the cultures do so I make up ideas based on what I’ve observed.  For all I know these microbes may be keeping the fungi clean and healthy, or maybe I’m missing out on some yields.  I imagine if a microbe is going to effect how your fungi colonizes the grain you’ll see at least signs in it in agar if not colonies, and if it’s not multiplying on agar to the point it’s noticeable you might not notice a difference all the way to the end.  Also, I’ve had to look very hard for these microbes, and there not always where I expect them to be.


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Offlinerumfor69
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Re: Enmeshed Bacteria? [Re: Land Trout]
    #28107507 - 12/21/22 03:18 PM (1 year, 1 month ago)

Interesting stuff and I appreciate the info. Bacteria having a mobility capability is especially intriguing.

See over here we have no pours that either wont or take forever to show signs of bacterial contamination. I even spit swabbed one of mine, it's still clear 4 days later. We're both using the 1/2 pint wide mouth jars and have a theory going along with the hypothetical I explained above.

I'm curious if this bacteria that is bad for our work, can some how move around, be undetected, and enmeshed in this hypothetical jar no pour dish situation then end up in your grain jar where it shows up just before 100% colonization. And you're transferring it to new no pour dishes where it can continue on doing what it does, even getting better at it maybe.

You end up with dishes like in my sig that still contaminate grains, and go on to be more dishes just like it.


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