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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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So I’ve got PE#6 going with some questions
#28073619 - 11/28/22 07:42 PM (1 year, 1 month ago) |
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I’ve read that it was never stabilized. Now from what I’ve read, genetics “reset” somewhat when coming from spore. But I’ve also had someone tell me generational learning is a thing.
I know they were introduced on agar with snake venom, im not so good as to understand the mechanics behind that. Tho I am interested if a good source explaining the mechanics are around.
My question I guess is do I have to clone? Such as find the mushrooms that have more of the PE genetics’ visible or can I collect spores from them?
Right now I can dump spawn to substrate and get fruits in a week. However they don’t grow tall, not really. I know later flushes increase size. And I plan to collect prints from each flush. I’m just wondering if I could better influence the direction of genetic material.
Would I be better off getting a microscope and doing slides? Introducing individual spores and isolating that way?
My gut feeling is that I’m going to be told to clone fruits.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: So I’ve got PE#6 going with some questions [Re: mushroomboy]
#28073645 - 11/28/22 07:54 PM (1 year, 1 month ago) |
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Do both. That way you keep the bare minimum growing (the clones) and can attempt to further stabilize the genetics (the spores).
Don't expect success, but if you don't give up and keep propagating the spores from the fruits you like the most, eventually they will either fully revert and you will not see any of the desired phenotypes or you will have a stable presentation that you are satisfied with and can call your own.
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Re: So I’ve got PE#6 going with some questions [Re: cooleko]
#28073771 - 11/28/22 09:50 PM (1 year, 1 month ago) |
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Thanks that’s super helpful. Next flush I’ll start clones, I’ll clone the stem in one box and do spores in the other. I know I’ll have to chase contamination with cloning. But that’s the game. This is super helpful!!!
I’m glad I don’t have to get into slides yet. I don’t have the equipment, it’s the one last thing I need. I’m working on an experimental flow hood. If that pans, next is a good microscope. Eventually I plan on a full diy lab.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 6 hours, 18 minutes
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Re: So I’ve got PE#6 going with some questions [Re: mushroomboy]
#28073836 - 11/28/22 11:07 PM (1 year, 1 month ago) |
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Quote:
mushroomboy said: Thanks that’s super helpful. Next flush I’ll start clones, I’ll clone the stem in one box and do spores in the other. I know I’ll have to chase contamination with cloning. But that’s the game. This is super helpful!!!
I’m glad I don’t have to get into slides yet. I don’t have the equipment, it’s the one last thing I need. I’m working on an experimental flow hood. If that pans, next is a good microscope. Eventually I plan on a full diy lab.
Actually clone material is far cleaner than anything else if you are using sterile processes. I have on many occasions taken a clone plate to grain.
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Re: So I’ve got PE#6 going with some questions [Re: DERRAYLD]
#28073848 - 11/28/22 11:27 PM (1 year, 1 month ago) |
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So I should start focusing on cloning and cleaning that up instead of prints? I’ve got PE and hopefully can clean up the jacks but I’d love to stabilize the PE#6s just for the shits.
Ugh ok, I’ll try and focus on cloning next flush.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 6 hours, 18 minutes
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Re: So I’ve got PE#6 going with some questions [Re: mushroomboy]
#28073855 - 11/28/22 11:34 PM (1 year, 1 month ago) |
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I don't really take spore prints unless I'm looking to go back to genetics to look for something new, I prefer cloning if I'm happy with the genetics presented.
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Re: So I’ve got PE#6 going with some questions [Re: DERRAYLD]
#28076707 - 11/30/22 06:01 PM (1 year, 1 month ago) |
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I’ll keep that in mind, things to think about.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
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