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OfflineBIG_Steve_01
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Fungi Imperfecti? * 1
    #28055477 - 11/16/22 11:14 PM (1 year, 2 months ago)

Hi guys! I recently harvested a batch of mushrooms (P.Cubensis), and noticed that there were not a lot of spores being discharged. I picked a smaller cap, cut the stem and stuck the cap to the inside of petri dish lid with double-sided and let whatever fell out of the gills land on agar. After letting the cap sit there for 2 hours, I viewed the agar under a dissecting microscope and noticed colorless, spore-looking things on the plate. I let it grow, and after a week, hyphae started to come out of the spore-looking things. 2 weeks in, the hyphae grew into what looked like very thin, mold-looking things. So I scraped some mycelium and dropped them in 5% Metol solution. Since metol reacts with psilocybin, if there's psilocybin in the mycelium the metol solution should turn purple pretty quickly, and it did. Then I made a slide out of it, and under the microscope, it seems like there are conidiophore like structure among the hyphae. This got me pretty confused, because as far as I know P.Cubensis don't reproduce asexually, I thought it must be something else and I found that the closest-looking thing to this are fungi in the Oedocephalum genus. Does anyone know what this might be? Is there an asexual state in P.Cubensis? are there any other compound that turns metol solutions purple? I'd like to hear what you guys think.


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Invisiblemurderlabz
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Re: Fungi Imperfecti? [Re: BIG_Steve_01]
    #28056940 - 11/17/22 07:17 PM (1 year, 2 months ago)

So strange kinda looks like Aspergillus sp.

https://wellcomecollection.org/works/hbcfz3zu/items


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OfflineBIG_Steve_01
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Re: Fungi Imperfecti? [Re: murderlabz]
    #28062394 - 11/20/22 08:26 PM (1 year, 2 months ago)

Yeah ikr, I thought of that too but this specimen lacks sterigma and conidia in chains.


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Invisiblemurderlabz
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Re: Fungi Imperfecti? [Re: BIG_Steve_01]
    #28062595 - 11/21/22 12:30 AM (1 year, 2 months ago)

Is the mycelium still growing out, are you able to take a look at another random area?


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InvisibleCreonAntigone
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Re: Fungi Imperfecti? [Re: murderlabz] * 1
    #28062630 - 11/21/22 01:29 AM (1 year, 2 months ago)

Yes psilocybin mushroom in the mycelial stage produce conidia and have conidiophores! I posted about this before and was surprised how few people knew.

But it's been scientifically documented.

The article 'Anamorphs in the Strophariaceae' by Grit Walther and Michael Weiss contains a picture and a description of the conidiophores and conidia of Psilocybe semilanceata. We may assume other psilocybe mushrooms are probably at least comparable.

Here is the picture; it does look similar to what you found in the OP.



Quote:

Psilocybe semilanceata (Fr.) P. Kumm.
MATERIAL STUDIED: GLM 46022, BAYER G844.
DESCRIPTION: (Fig. 17). Macroscopic, mat white to pale orangeochre, cottony to felted. Microscopic, only haploid mycelia investigated, haploid mycelia displacing dikaryotic mycelia in polysporic cultures; up to 4 ch condensing (number inferred for young conidiophores); conidia straight to curved, 2.0–8.0 mm " 1.1–2.0 mm, with one to several, minute to
medium-sized intracellular droplets, or without intracellular
droplets.




For further study I suggest you consult the article, "The systematic relevance of conidiogenesis modes in the gilled Agaricales" by Sigisfredo Garnica includes an in-depth study with drawings of the conidiophores in asexual modes of mushrooms in that group, including some psilocybe species (all of which are Agaricales).


Edited by CreonAntigone (11/21/22 01:53 AM)


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Invisiblemurderlabz
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Re: Fungi Imperfecti? [Re: CreonAntigone]
    #28063297 - 11/21/22 12:58 PM (1 year, 2 months ago)

Quote:

CreonAntigone said:
Yes psilocybin mushroom in the mycelial stage produce conidia and have conidiophores! I posted about this before and was surprised how few people knew.





Wunderbar! Thank you for dropping the knowledge.


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Offlinetrippleblack
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Re: Fungi Imperfecti? [Re: murderlabz]
    #28076676 - 11/30/22 05:49 PM (1 year, 1 month ago)

Thanks for sharing.  Looks like conidiospores to me..  I notice other things i have not found in the literalture too. such as the ability to produce and utilize fuctions such as anteridial and oogonium gamete structures. i can only imagine not a ton of people are spending hours and hours in front of a scope.


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