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InvisibleMercent
Troglodyte


Registered: 11/10/22
Posts: 69
Loc: North Korea Flag
Research summary of Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis * 1
    #28064224 - 11/22/22 01:13 AM (1 year, 2 months ago)

Stability of psilocybin and its four analogs in the biomass of the
psychotropic mushroom Psilocybe cubensis (Kuchar.M 2020).

Link: https://sci-hub.se/https://doi.org/10.1002/dta.2950

I don't know if this has been posted. I hope this will put an end to the debate of drying temperature and storage conditions (or maybe it wouldn't if you choose to nitpick the methodology).

Summary:

1. Conversion of Psilocybin into Psilocin at increasing temperature; Psilocin more prone to oxidative degradation. Drying at 25°C is the best; but is not not much the different than drying at 75°C; rapid degradation past 100°C.



"Degradation of tryptamines was observed at 25°C, 50°C, 75°C, 100°C, 125°C, and 150°C for 30 minutes according to the procedure attached in the supporting information...An exception to these results was psilocin, where the concentration increased at higher temperatures. The loss of phosphorylated analytes and at the same time the increased amount of psilocin may be explained by the dephosphorylation of tryptamines to their hydrolyzed forms, which are easily subjected to oxidative degradation."

2. Storing fresh mushroom in freezer (-20°C) is bad.

"When storing mushrooms in the freezer (-20°C, -80°C), significantly lower
concentrations of all of the analytes were detected except for psilocin. The most pronounced decay occurred at -80°C, where 94 % less psilocybin was measured than for samples stored in the dark at room temperature"

"For all of the samples stored in the freezer, the concentration of phosphorylated analytes (the main psilocybin) was reduced."

3. Dried mushroom powder loses potency rapidly; storing in the dark at low temp (20°C) is best. Storing in fridge (4°C) or freezer (-20°C) is bad. Light exposure is bad.



4. Stipe and cap has no significant difference in alkaloids

'There was approximately 50 % less baeocystin, psilocybin, and norbaeocystin in the stipes than in the caps. The stipes contained 32 % less aeruginascin and 85 % less psilocin than the caps. The total content of tryptamine alkaloids in the stipes was approximately 50 % less than in the caps....The parametric two-sample unpaired t-test with Welch correction in the R program found that the results were not statistically significant, as the p-value was 0.3756.'

5. Fresh mushroom is 90% water, less than 0.87% alkaloids

'In conclusion, the water content in the fresh mushrooms was approximately 90 %, which is in agreement with another mushroom study and the concentrations of tryptamines were as follows: 0.54 wt.% of psilocybin, 0.02 wt.% of
norbaeocystin, 0.01 wt.% of aeruginascin, 0.05 wt.% of baeocystin, and 0.25 wt.% of psilocin.'


Edited by Mercent (11/22/22 01:15 AM)


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Offlinetriptamine
Navagator

Registered: 02/06/02
Posts: 73
Loc: SW
Last seen: 1 day, 19 hours
Re: Research summary of Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cu... [Re: Mercent]
    #28073422 - 11/28/22 05:14 PM (1 year, 1 month ago)

Looks like good research, but I take some issue with the freezer results.  Many have kept dried mushrooms in the freezer for 5+ years with minimal potency loss.  That said, in this study, the mushrooms were powdered.  I wonder if they had open containers of powder and condensation/evaporation took place?


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OfflineDharmaForKarma
Tub monkey
Male

Registered: 04/24/20
Posts: 565
Last seen: 1 day, 6 hours
Re: Research summary of Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cu... [Re: triptamine]
    #28074091 - 11/29/22 07:01 AM (1 year, 1 month ago)

It’s a not a high quality study. Here is another link:

https://www.oregon.gov/oha/PH/PREVENTIONWELLNESS/Documents/Stability%20of%20Psilocybin%20and%20Analogs.pdf

I commented on it last year: 

https://www.shroomery.org/forums/showflat.php/Number/27596389#27596389

The summary seems to confirm what this community already knows. Dry quickly, dry thoroughly, store in the dark with low humidity, and eliminate air exchange. Dark is better than light (note for the sun and air driers reading). Powder degrades quickly in the presence of air.


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OfflineDharmaForKarma
Tub monkey
Male

Registered: 04/24/20
Posts: 565
Last seen: 1 day, 6 hours
Re: Research summary of Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom… [Re: Mercent] * 1
    #28074096 - 11/29/22 07:09 AM (1 year, 1 month ago)

Quote:

Mercent said:
I hope this will put an end to the debate of drying temperature and storage conditions…




Never lose hope. But the “debate” will never die because it depends on reading. The common tendency to baby our harvested fruits just as we baby spores-to-mycelium is regrettable. Descriptions of “drying gently”…. Right up there with fanning tubs and adding weird shit to substrates for “nutrition.”


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InvisibleMercent
Troglodyte


Registered: 11/10/22
Posts: 69
Loc: North Korea Flag
Re: Research summary of Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cu... [Re: DharmaForKarma]
    #28075510 - 11/30/22 06:00 AM (1 year, 1 month ago)

Indeed. I have been reading through a lot of post on proposed drying temperature. Many people claim that drying it fast under high temperature is better. But it would seem that drying it in low temperature is better, though not that much better.

As for the freezer result, i imagine ice crystals are to be blamed. Ice crystals absolutely poke holes in your sample and during thawing, i bet there are much loss of psilocybin thanks to the altered sample, especially if their test is not doing immediately after thawing.

I wonder if you leave your frozen shrooms on the counter for a few hours after thawing, exposed to the environment, will that affect your experience?


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