Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Offlinemushroomboy
Mushroom King
Other User Gallery
Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
So I’ve got PE#6 going with some questions
    #28073619 - 11/28/22 07:42 PM (1 year, 1 month ago)

I’ve read that it was never stabilized. Now from what I’ve read, genetics “reset” somewhat when coming from spore. But I’ve also had someone tell me generational learning is a thing.

I know they were introduced on agar with snake venom, im not so good as to understand the mechanics behind that. Tho I am interested if a good source explaining the mechanics are around.

My question I guess is do I have to clone? Such as find the mushrooms that have more of the PE genetics’ visible or can I collect spores from them?

Right now I can dump spawn to substrate and get fruits in a week. However they don’t grow tall, not really. I know later flushes increase size. And I plan to collect prints from each flush. I’m just wondering if I could better influence the direction of genetic material.

Would I be better off getting a microscope and doing slides? Introducing individual spores and isolating that way?

My gut feeling is that I’m going to be told to clone fruits.


--------------------
int getRandomNumber()
{
return 4; // Chosen by random dice roll
}; // guaranteed to be random. <3 GeoHot


Extras: Filter Print Post Top
Invisiblecooleko
Augmentum provocatus
 User Gallery
Registered: 08/15/22
Posts: 1,010
Loc: Seattle
Re: So I’ve got PE#6 going with some questions [Re: mushroomboy]
    #28073645 - 11/28/22 07:54 PM (1 year, 1 month ago)

Do both. That way you keep the bare minimum growing (the clones) and can attempt to further stabilize the genetics (the spores).

Don't expect success, but if you don't give up and keep propagating the spores from the fruits you like the most, eventually they will either fully revert and you will not see any of the desired phenotypes or you will have a stable presentation that you are satisfied with and can call your own.


Extras: Filter Print Post Top
Offlinemushroomboy
Mushroom King
Other User Gallery
Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
Re: So I’ve got PE#6 going with some questions [Re: cooleko]
    #28073771 - 11/28/22 09:50 PM (1 year, 1 month ago)

Thanks that’s super helpful. Next flush I’ll start clones, I’ll clone the stem in one box and do spores in the other. I know I’ll have to chase contamination with cloning. But that’s the game. This is super helpful!!!

I’m glad I don’t have to get into slides yet. I don’t have the equipment, it’s the one last thing I need. I’m working on an experimental flow hood. If that pans, next is a good microscope. Eventually I plan on a full diy lab.


--------------------
int getRandomNumber()
{
return 4; // Chosen by random dice roll
}; // guaranteed to be random. <3 GeoHot


Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 6 hours, 18 minutes
Trusted Cultivator
Re: So I’ve got PE#6 going with some questions [Re: mushroomboy]
    #28073836 - 11/28/22 11:07 PM (1 year, 1 month ago)

Quote:

mushroomboy said:
Thanks that’s super helpful. Next flush I’ll start clones, I’ll clone the stem in one box and do spores in the other. I know I’ll have to chase contamination with cloning. But that’s the game. This is super helpful!!!

I’m glad I don’t have to get into slides yet. I don’t have the equipment, it’s the one last thing I need. I’m working on an experimental flow hood. If that pans, next is a good microscope. Eventually I plan on a full diy lab.




Actually clone material is far cleaner than anything else if you are using sterile processes.
I have on many occasions taken a clone plate to grain.


Extras: Filter Print Post Top
Offlinemushroomboy
Mushroom King
Other User Gallery
Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
Re: So I’ve got PE#6 going with some questions [Re: DERRAYLD]
    #28073848 - 11/28/22 11:27 PM (1 year, 1 month ago)

So I should start focusing on cloning and cleaning that up instead of prints? I’ve got PE and hopefully can clean up the jacks but I’d love to stabilize the PE#6s just for the shits.

Ugh ok, I’ll try and focus on cloning next flush.


--------------------
int getRandomNumber()
{
return 4; // Chosen by random dice roll
}; // guaranteed to be random. <3 GeoHot


Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 6 hours, 18 minutes
Trusted Cultivator
Re: So I’ve got PE#6 going with some questions [Re: mushroomboy]
    #28073855 - 11/28/22 11:34 PM (1 year, 1 month ago)

I don't really take spore prints unless I'm looking to go back to genetics to look for something new, I prefer cloning if I'm happy with the genetics presented.


Extras: Filter Print Post Top
Offlinemushroomboy
Mushroom King
Other User Gallery
Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
Re: So I’ve got PE#6 going with some questions [Re: DERRAYLD]
    #28076707 - 11/30/22 06:01 PM (1 year, 1 month ago)

I’ll keep that in mind, things to think about.


--------------------
int getRandomNumber()
{
return 4; // Chosen by random dice roll
}; // guaranteed to be random. <3 GeoHot


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Simple Dessicant Question NugDumper 1,709 5 06/12/02 11:57 AM
by DinoMyc
* Re: Genetically Mutated Mushrooms
( 1 2 3 all )
mycofile 14,261 47 12/28/99 12:48 PM
by Anonymous
* HELP...Questions for Experianced Bulk Growers??? Angel_Magic79 2,125 8 05/25/02 10:53 AM
by strang
* Genetics of Mushrooms... angryshroom 2,827 17 06/02/02 03:17 AM
by r05c03
* Mycelium & Genetic Exchange Gawain 3,226 13 10/23/02 09:42 PM
by Anonymous
* Stabilization ? Ashana 411 1 07/07/03 04:10 PM
by micro
* spawing and genotype question... sluglee 1,097 6 11/11/02 03:05 PM
by tchyted
* PES confusion
( 1 2 all )
pleezr 5,690 20 03/07/02 09:36 PM
by mycofile

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
308 topic views. 1 members, 3 guests and 5 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.007 seconds on 14 queries.