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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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New flow hood build
#28070666 - 11/26/22 07:26 PM (1 year, 2 months ago) |
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So I’ve got this flow hood setup I’ve been working on. It’s basically a tote with a basic pre-filter with a H14 in a clear tote. The fans are 89-120cfm, unsure of the static pressure of the filter but the fans can handle it.

I’ve herd a flame test works, should be 90 deg from the filter. Anyone experienced enough to say yes or no? I’ve used it once and those samples seem good.
If it looks too weak I can order new/stronger delta fans. If I do that I might get a new pre-filter, I have an H13 that would do well.
Thiughts?
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Edited by mushroomboy (11/26/22 07:27 PM)
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DCAE
Base


Registered: 02/21/20
Posts: 10,242
Loc: somewhere in eurasia
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Edited by DCAE (12/05/22 08:28 PM)
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 23 days, 18 hours
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You're using a plastic tote as the main box for the hood? Can we see more pics? I just see a lot of potential issues here...
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,218
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Re: New flow hood build [Re: Forrester] 3
#28071108 - 11/27/22 06:03 AM (1 year, 1 month ago) |
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Advanced Mycology
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murderlabz
RIP Stoneman



Registered: 05/18/19
Posts: 550
Loc: The Multiverse
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H14, which filter are you using? it looks like a high flow...
High flows don't build the static pressure that you need for laminar flow. With out laminar flow it would have turbulence pulling in dirty air from the sides blasting your work.
Not judging, just don't want to see your hard work ruined.
Edited by murderlabz (11/27/22 11:24 AM)
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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I’ve thought about ways to control flow. I’ve added my shield.

I’ve also upped the fan voltage from 12v to 13.5 and might go up to 15 but it’s brushless…. Don’t want to burn them out either. Delta fans aren’t cheap.
If I get poor results suggesting turbulence, I’m going to increase fan speed and add in a way to equalize flow. Which would be adding another chamber after the filter with positive airflow and then moving that towards a slightly smaller outlet with a lightweight filter to eventually disperse flow over an area.
But I have faith this is getting good even flow. As I built it with a positive air chamber after the fans. It’s best for fans to push with positive pressure. Better efficiency and airflow, fans push not pull.
I’ve been in the overclocking/computer mod scene for a while. These are good delta fans AFC1212D, good rated fans.
The filter is definitely not designed for this, that’s the whole purpose of the experiment. Can I get a filter to work that’s not designed for such tasks to save money. It’s the Medify MA-40 Genuine Replacement Filter off Amazon. I chose it because of the price and specs.
Last week (11/20) I did some 60mm and 75mm dishes. I expected bacterial contamination. I got one without noticeable bacteria and no other contamination.

I’m doing more batches and keeping track ish of them. I’m running out of room. :/ ugh and the source jar for ALL these transfers are these two badly contaminated specimens.
I posted about Jack frosts like a while ago, being a colorblind fuck I didn’t know how dark grey it really was.

This is the original source, which I open air collected this:

Transferred it to this:

The small 4oz agar definitely has bacteria, as one can see the black contamination. I’ve painstakingly cleaned that up to 5 clean agars in my glove box. Yes I know there is big debate about that shit. I use a glove box and do well, I’m not arguing that shit.
So after countless nights of pressure cooking agar and waiting for the glove box to settle… I do an hour wait, heavy soap solution, works for me. Built this out of boredom.
I understand some thermodynamics, air fluid dynamics, shit like that. Drag, what causes turbulence. This was supposed to be a clean air glove box but that didn’t work out so now I made it a side flow hood. And I’m pretty sure flow is even but probably only 200 cfm. Maybe 250, doubtful.
Ya that’s about all the current info needed. So don’t worry this isn’t my only way to do serious sterile work, it’s just a side project to hopefully speed shit up. I’m only running contaminated agar runs on it. None of my 100% clean until I get more results.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Re: New flow hood build [Re: Forrester]
#28073584 - 11/28/22 07:19 PM (1 year, 1 month ago) |
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See my post above. And everything is sealed, riveted and caulked. Tape is extra. I’ve posted the fans and filter as well as more of the finished setup.
Edit: to bakedbeings
Since this has to do with fluid dynamics, airflow and competent filters I didn’t think this would be good for general mycology. On top of the topic of the fans used, using delta fans instead of a blower style input is definitely an oddity. Unless you understand such nuances it’s not exactly a beginner topic but I can re-post elsewhere if a mod wants or to move the post.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
Edited by mushroomboy (11/28/22 07:22 PM)
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murderlabz
RIP Stoneman



Registered: 05/18/19
Posts: 550
Loc: The Multiverse
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Not sure what the last xxxx are of your AFC1212D Deta's. But here is the AFC1212D-SP19 data sheet for your fans which gives the static pressure graph.
https://www.y-ic.com/datasheet/ad/ATS-20B-167-C3-R0.pdf
Godspeed with the experimenting.
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Oh nice, that’ll help.

That’s the fan info, all I got.
I’ll put this into good use.
Edited by mushroomboy (11/28/22 09:47 PM)
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murderlabz
RIP Stoneman



Registered: 05/18/19
Posts: 550
Loc: The Multiverse
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Quote:
mushroomboy said: Oh nice, that’ll help.

That’s the fan info, all I got.
I’ll put this into good use.
You have some -F00
Here you go https://www.delta-fan.com/Download/Spec/AFC1212D-F00.pdf
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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The main reason I chose the filter was it’s the cheapest that touts reasonable specs.
I get what you mean by filter type, deep filters produce more laminar flow. Otherwise you have to guide and manipulate the air stream. Which I’m fine doing.
So far by adding the shield and flat base its producing decent flow in the amount of space I require. I might just take that plexiglass and build a permanent work space. Use that wire grill for my larger glove box so I can stop using my dehydrator racks lol.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
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jaycrayzy
Xombie Boy


Registered: 08/22/13
Posts: 73
Loc: Under the bed
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I mean if it works, it works, but I'm pretty damn sure you won't achieve laminar flow from that. I feel like the force of the fan might separate the fibers in the filter causing the size particles it let's through to be larger, which means it's not clean air either.
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Re: New flow hood build [Re: jaycrayzy]
#28077382 - 12/01/22 01:10 AM (1 year, 1 month ago) |
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Right, I’m pushing fan and filter beyond specs. Sure. But that’s the point, can I.
I’ve got two glove boxes which work with much efficiency. But between them and waiting….. Pressure cooker time, this happened.
Right now the filter works. For how long? Idk.
This isn’t a serious project, it’s just to see what I can push Amazon spec shit.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
Edited by mushroomboy (12/01/22 01:11 AM)
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jaycrayzy
Xombie Boy


Registered: 08/22/13
Posts: 73
Loc: Under the bed
Last seen: 1 year, 1 month
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I get it. I found an ffu on craigslist for $125 a few weeks ago. It's not laminar but it works pretty damn well. I plan to upgrade the fan motor and get one of those 5-7/8" thick laminar flow filters for it soon though.
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Jay Kay the Xombie
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Re: New flow hood build [Re: jaycrayzy]
#28077400 - 12/01/22 02:14 AM (1 year, 1 month ago) |
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Dude if this is as good as specs say. It’s god damn fire. Let me get original transfer pictures today
Edit: these were done without plexiglass flow

I’m doing more
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
Edited by mushroomboy (12/01/22 02:25 AM)
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mushroomboy
Mushroom King

Registered: 09/21/11
Posts: 155
Last seen: 2 days, 8 hours
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Update: save the blue meanies project is going well. I originally inoculated in my glove box but all work prior has been done in my flow hood.

The original agar I scraped spores out of a syringe from. Done in my glove box.

The samples I have going. I now know to make my agar thinner, next batch of agar should be much better.
-------------------- int getRandomNumber() { return 4; // Chosen by random dice roll }; // guaranteed to be random. <3 GeoHot
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