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Offlineodissey
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Registered: 01/19/21
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Re: A strain crossing thread [Re: multifractal]
    #27639288 - 01/31/22 06:59 AM (2 years, 16 days ago)

Cool! I will try it out :mushroom2:


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Invisiblebigfootscreepyuncl
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Re: A strain crossing thread [Re: odissey] * 1
    #27752783 - 04/26/22 11:32 PM (1 year, 9 months ago)

More or less just popping in to say Hi and follow along. I've got a couple projects I'd like to get going so whether you like it or not I'll be hanging around this thread :lol:


@faht - congrats (again) on your FP+ :rockon: would you say your 'double swab, rub together in sterile water' method is 'proven' at this point? Once you make that spore solution I'm assuming you just make your own syringe and go to agar/cake/grain?

Sorry if this has already been covered but I just came across this thread and haven't started researching yet


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Edited by bigfootscreepyuncl (04/26/22 11:53 PM)


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InvisiblefahtsterM
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Re: A strain crossing thread [Re: bigfootscreepyuncl] * 1
    #27753278 - 04/27/22 11:36 AM (1 year, 9 months ago)

I mean, I’m confident on at least 4 PE crosses that they’re successful.. so I guess proven to that degree.. where it gets to be questionable is when you’re using varieties that aren’t obvious.. like the PE/pesa I did, I’m still not a 100% confident I grabbed a cross fruit because dry there isn’t obvious traits in pesa to set apart from the PE.. but PE/Aa+, PE/RW, PE/apeu, PE/pfrs im as confident in ever going to be it’s successful.

The key is using All the SS and growing it out


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Invisiblebigfootscreepyuncl
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Re: A strain crossing thread [Re: fahtster] * 1
    #27753296 - 04/27/22 11:57 AM (1 year, 9 months ago)

Thanks for taking the time to answer yet another one of my vague and poorly worded questions :lol:

Looks like I should practice making syringes before I get too caught up in the actual crossing. Thanks!


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OfflineA.k.aM
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Re: A strain crossing thread [Re: bigfootscreepyuncl] * 1
    #27753801 - 04/27/22 06:15 PM (1 year, 9 months ago)

You could always just streak plates too, faht somehow manages to do everything without agar :shrug2:


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InvisibleTheConfluence
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Re: A strain crossing thread [Re: A.k.a]
    #27753907 - 04/27/22 07:16 PM (1 year, 9 months ago)

What the fuck?
That's nuts... Unless it's spore straight to liquid.


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When two people dream the same dream, it ceases to be an illusion.


Philip K. Dick
Lies, Inc. (1984)



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InvisiblefahtsterM
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Re: A strain crossing thread [Re: TheConfluence] * 2
    #27754077 - 04/27/22 08:38 PM (1 year, 9 months ago)

I make SS out of swabs and then inoculate pf cakes and/or 4oz grain jars (multiple of both) and the best looking one of the 4oz grain jars, I’ll GLC to qts and spawn the glc grains to sandwich baggie subs.. that’s only if I have to.. if the fruits drop spores, it’s much easier because I’ll make Jar prints that I’ll take straight to grain… I haven’t one of the jar prints fail going straight to grain yet

Unfortunately I prefer varieties that don’t drop spores since they tend to be more potent.  Spores to grain is risky but the trick is to use small amounts of SS on many small substrates.. then if I like the culture, I’ll clone straight to grain and dry the culture out on grain for storage.  It’s wacky to everyone else but to me it’s a Wednesday :lol:


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OfflineHindsight
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Re: A strain crossing thread [Re: fahtster] * 1
    #27754278 - 04/27/22 10:04 PM (1 year, 9 months ago)

Not whacky to me - just serves to dramatically speed up the generational cycles. Love your methods!


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InvisibleSmartattack
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Re: A strain crossing thread [Re: fahtster]
    #27754292 - 04/27/22 10:14 PM (1 year, 9 months ago)

Quote:

fahtster said:
I make SS out of swabs and then inoculate pf cakes and/or 4oz grain jars (multiple of both) and the best looking one of the 4oz grain jars, I’ll GLC to qts and spawn the glc grains to sandwich baggie subs.. that’s only if I have to.. if the fruits drop spores, it’s much easier because I’ll make Jar prints that I’ll take straight to grain… I haven’t one of the jar prints fail going straight to grain yet

Unfortunately I prefer varieties that don’t drop spores since they tend to be more potent.  Spores to grain is risky but the trick is to use small amounts of SS on many small substrates.. then if I like the culture, I’ll clone straight to grain and dry the culture out on grain for storage.  It’s wacky to everyone else but to me it’s a Wednesday :lol:






:awepreciation:


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OfflineRotnpins
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Re: A strain crossing thread [Re: cronicr]
    #27754450 - 04/28/22 02:23 AM (1 year, 9 months ago)

:popcorn:


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OfflineHuskies
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Re: A strain crossing thread [Re: Rotnpins]
    #27799322 - 05/30/22 05:12 PM (1 year, 8 months ago)

:havesomescience:

Albino Cambodians are coming soon, then I have Leucistic PE which despite being slow, has a DOPE looking master plate.

Perhaps I'll mix them with my Husky mystery genetics and create a proper "Strain"

I swear, there was a plate that I did way back, that grew a wall between two varieties, but  ONE bridge did come through.

I tried sampling, got bacteria. Probably was due to bacteria tbh. snience


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OfflineLand TroutM
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Re: A strain crossing thread [Re: Huskies] * 1
    #27861862 - 07/13/22 10:09 PM (1 year, 7 months ago)

hey y'all


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Invisiblethe_chosen_one
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Re: A strain crossing thread [Re: Land Trout] * 7
    #27864794 - 07/16/22 12:59 PM (1 year, 6 months ago)

Several generations later and the Falbino Chimera project seems to be stabilizing. It's disappointing not to see an albino in a while, but it's still lurking in there. Either way it's a nice little mushroom with deceptively solid stems.







It's also a tenacious little shit that won't let go of the sub and grows all over it. :lol:
We'll see if it survives harvest.


:awesome2:


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Offlinecozmyc
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Re: A strain crossing thread [Re: the_chosen_one] * 1
    #27864870 - 07/16/22 02:12 PM (1 year, 6 months ago)

Some beautiful work in here. :popcorn:


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OfflineBagOfDicks
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Re: A strain crossing thread [Re: cozmyc] * 1
    #27865176 - 07/16/22 06:19 PM (1 year, 6 months ago)

:whathesaid:    :popcorn:


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InvisibleAyePlusS
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Re: A strain crossing thread [Re: BagOfDicks]
    #28066931 - 11/24/22 03:55 AM (1 year, 2 months ago)

Bumping for awesomeness

Just mixed aa+ and Melmac (i know i know its been done (killing it faht and eats) but i want big white monsters) thought is to backcross into aa after i get something fatty, but we’ll see where it goes

And gonna give a shot at backcrossing melmac into swpe just for funzies

Used spore swabs for all, cut off tips with scalpel and dropped into jars with 10 cc’s sterilized h2o, shook the shit outta them and sucked up into syringes

Shot a few cc’s on 4 jars of grain each and a couple drops on agar plates in case my swabs are dirtier than i think


Gf is outta town and I can’t sleep, it’s Probably not gonna come to shit but never hurts to try right?


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Invisiblethe_chosen_one
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Re: A strain crossing thread [Re: AyePlus] * 4
    #28068666 - 11/25/22 11:15 AM (1 year, 2 months ago)

Should have bought a Lotto ticket that day. PFR x PE crossed on grain. Had a smidge of solution left from both and injected into one jar for grins. Looks entirely different than the parents and even Fahts cross. Took a print from the leucistic fruit front and center to take to further generations.






:awesome2:


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"Luck favors the observant." - Workman



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