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Offlinehossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
LC carmalization
    #12164950 - 03/08/10 02:27 PM (13 years, 11 months ago)

so i did a buncha jars with light malt extract, i think i did to high a mix....1 tablespoon per cup of water, but anyway there was alot of carmalization after the PC so theres a lot of particles floating around in it...and i cant tell if the small masses that have formed are contam...or the culture, i inoculated these with 1cc each of a spore syringe i got online, even with all the particles can spores still germ in that enviroment?



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Invisiblebare.whiterabbit
Lapin Blanc
I'm a teapot User Gallery


Registered: 11/29/09
Posts: 912
Loc: The Microwave Flag
Re: LC carmalization [Re: hossfield]
    #12165098 - 03/08/10 02:51 PM (13 years, 11 months ago)

you don't need nearly that much, man...1-4% of the volume of water...

I only use MAYBE a teaspoon in my 250ml LCs...

warm the water first by nuking it, then add your malt and stir till it's nicely disolved, then filter through a gold coffee filter or metal mesh coffee filter, into whatever container you are using and PC for 20 min at 15 psi...you get there by boiling the PC water first, throw the lid and weight on, then wait till your jiggler or whatever starts going crazy/spitting steam (if you can keep it from spitting it's better, but you have to learn your PC), then turn it down to about the second or third notch on your stove and let it sit...

Here's the thing...I'd start counting down your 20min, when you first put your lid/weight on, because it's already boiling inside the PC when on...any more than that and I think you may over-cook them


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Offlinehossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
Re: LC carmalization [Re: bare.whiterabbit]
    #12165182 - 03/08/10 03:05 PM (13 years, 11 months ago)

i know i put to much in i said that, im wondering if this is contam or what, i want to salvage the strain since i used the whole syringe for all the cultures....which were all 1 tablespoon per cup water...what im hoping to do is transfer them to a diff batch i just made (2 teaspoons/quart water)....these are cooling and have no carmalization in em...i just dont want to reorder a another syringe...will the myc still grow in the stronger/carmalized mix? or am what i seeing contam?


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trust the fungus


Edited by hossfield (03/08/10 03:05 PM)


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Invisiblemyceleus_rex
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Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: LC carmalization [Re: hossfield]
    #12165233 - 03/08/10 03:14 PM (13 years, 11 months ago)

Another option is raw sugar, about 5g. per 100ml.  It's quite resistant to carmelization.  It works far better than karo and is close to malt/dex plus it's very clear, no filtering needed. IME it grows myc as thick as possibly necessary.
Quote:

hossfield said:
i know i put to much in i said that, im wondering if this is contam or what, i want to salvage the strain since i used the whole syringe for all the cultures....which were all 1 tablespoon per cup water...what im hoping to do is transfer them to a diff batch i just made (2 teaspoons/quart water)....these are cooling and have no carmalization in em...i just dont want to reorder a another syringe...will the myc still grow in the stronger/carmalized mix? or am what i seeing contam?




You used a whole syringe to make LCs?


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Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


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Invisiblebare.whiterabbit
Lapin Blanc
I'm a teapot User Gallery


Registered: 11/29/09
Posts: 912
Loc: The Microwave Flag
Re: LC carmalization [Re: myceleus_rex]
    #12165535 - 03/08/10 04:17 PM (13 years, 11 months ago)

Try transferring to the less sugar'd batch and see if it takes off...if not, I'd even just PC some water to sterility and transfer to it...enough sugar with probably come with to support it


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Offlinehossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
Re: LC carmalization [Re: bare.whiterabbit]
    #12165896 - 03/08/10 05:14 PM (13 years, 11 months ago)

i made 1 pf cake and 6 LCs( 3 honey, 3 light malt)....they look like the puffy little white myc balls...just with a buncha particles stuck all throughout it....im just worried cause ive had a ton of trouble with trich in the past...what does that look like in a LC?


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Offlinehossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
Re: LC carmalization [Re: hossfield]
    #12169404 - 03/09/10 09:15 AM (13 years, 11 months ago)

anyone know will trich look like mycellium in a liquid culture?any have pics?


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Offlinekiyote
Apprentice Grower
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Registered: 07/08/08
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Re: LC carmalization [Re: hossfield]
    #12169613 - 03/09/10 10:06 AM (13 years, 11 months ago)

The major problem with LCs is that you don't know if they're contaminated until you knock up a bunch of jars and they all get infected.  It's the risk you take with this method of making them :shrug:

There is another LC method where you take a quart jar of grain, let it get fully colonized and then inject water into the mix, and suck it out with a syringe.  That way you can see if there are contams before turning it into a liquid culture.


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Invisibleandymc
cocoa beetles from zanzibar
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Registered: 11/25/09
Posts: 2,395
Re: LC carmalization [Re: hossfield]
    #12170122 - 03/09/10 11:42 AM (13 years, 11 months ago)

A few of my LCs have the little puffballs as well.  I'd toss them but it looks suspiciously like healthy mycelium, and there are no other obvious signs of contam.  I know people say you just have to try it (I inocced with one of them so we'll see what happens) but I'd be curious to know if anyone else has had success with an LC that had the puffballs.


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How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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Invisiblemyceleus_rex
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Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: LC carmalization [Re: kiyote]
    #12171041 - 03/09/10 02:01 PM (13 years, 11 months ago)

Quote:

kiyote said:
The major problem with LCs is that you don't know if they're contaminated until you knock up a bunch of jars and they all get infected.  It's the risk you take with this method of making them :shrug:

There is another LC method where you take a quart jar of grain, let it get fully colonized and then inject water into the mix, and suck it out with a syringe.  That way you can see if there are contams before turning it into a liquid culture.



That technique is linked in mi sig :thumbup:


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


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Offlinehossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
Re: LC carmalization [Re: myceleus_rex]
    #12174060 - 03/09/10 09:26 PM (13 years, 11 months ago)

hmmm i wonder if i could do that tech with flour/verm mix? i did do one flour/verm cake that directly got the spores...


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Invisibleandymc
cocoa beetles from zanzibar
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Registered: 11/25/09
Posts: 2,395
Re: LC carmalization [Re: andymc]
    #12194901 - 03/13/10 12:28 PM (13 years, 11 months ago)

Quote:

andymc said:
A few of my LCs have the little puffballs as well.  I'd toss them but it looks suspiciously like healthy mycelium, and there are no other obvious signs of contam.  I know people say you just have to try it (I inocced with one of them so we'll see what happens) but I'd be curious to know if anyone else has had success with an LC that had the puffballs.




I'll answer my own question, for the benefit of others.  Puffballs do not seem to indicate a failed LC.  I guess sometimes the myc just does that.  I inocculated a couple of jars from LCs that have puffballs, and the jars appear to be colonizing away.

So there we go.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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OfflineDinGusFunGus
Stranger

Registered: 11/21/22
Posts: 1
Last seen: 1 year, 2 months
Re: LC carmalization [Re: andymc]
    #28063142 - 11/21/22 11:48 AM (1 year, 2 months ago)

Hey man each of those floating fuzzy balls was one pair of Spores. This happens when making LCs from mss. Anyway you probably know by now whether it was good or bad.


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OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,317
Last seen: 3 hours, 16 minutes
Re: LC carmalization [Re: DinGusFunGus]
    #28063153 - 11/21/22 11:52 AM (1 year, 2 months ago)

Quote:

DinGusFunGus said:
Hey man each of those floating fuzzy balls was one pair of Spores. This happens when making LCs from mss. Anyway you probably know by now whether it was good or bad.



Just woke up from a nap and the first thread is a 12 year old bump. Good looks bro!


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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: LC carmalization [Re: Screwup] * 1
    #28063168 - 11/21/22 11:55 AM (1 year, 2 months ago)

We can all remain safe and sleep well at night knowing screwup is surely always on the case :thumbup:


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