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hossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
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LC carmalization
#12164950 - 03/08/10 02:27 PM (13 years, 11 months ago) |
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so i did a buncha jars with light malt extract, i think i did to high a mix....1 tablespoon per cup of water, but anyway there was alot of carmalization after the PC so theres a lot of particles floating around in it...and i cant tell if the small masses that have formed are contam...or the culture, i inoculated these with 1cc each of a spore syringe i got online, even with all the particles can spores still germ in that enviroment?
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trust the fungus
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bare.whiterabbit
Lapin Blanc



Registered: 11/29/09
Posts: 912
Loc: The Microwave
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Re: LC carmalization [Re: hossfield]
#12165098 - 03/08/10 02:51 PM (13 years, 11 months ago) |
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you don't need nearly that much, man...1-4% of the volume of water...
I only use MAYBE a teaspoon in my 250ml LCs...
warm the water first by nuking it, then add your malt and stir till it's nicely disolved, then filter through a gold coffee filter or metal mesh coffee filter, into whatever container you are using and PC for 20 min at 15 psi...you get there by boiling the PC water first, throw the lid and weight on, then wait till your jiggler or whatever starts going crazy/spitting steam (if you can keep it from spitting it's better, but you have to learn your PC), then turn it down to about the second or third notch on your stove and let it sit...
Here's the thing...I'd start counting down your 20min, when you first put your lid/weight on, because it's already boiling inside the PC when on...any more than that and I think you may over-cook them
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hossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
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i know i put to much in i said that, im wondering if this is contam or what, i want to salvage the strain since i used the whole syringe for all the cultures....which were all 1 tablespoon per cup water...what im hoping to do is transfer them to a diff batch i just made (2 teaspoons/quart water)....these are cooling and have no carmalization in em...i just dont want to reorder a another syringe...will the myc still grow in the stronger/carmalized mix? or am what i seeing contam?
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trust the fungus
Edited by hossfield (03/08/10 03:05 PM)
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myceleus_rex
seeker



Registered: 07/01/09
Posts: 1,581
Loc: alized
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Re: LC carmalization [Re: hossfield]
#12165233 - 03/08/10 03:14 PM (13 years, 11 months ago) |
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Another option is raw sugar, about 5g. per 100ml. It's quite resistant to carmelization. It works far better than karo and is close to malt/dex plus it's very clear, no filtering needed. IME it grows myc as thick as possibly necessary.
Quote:
hossfield said: i know i put to much in i said that, im wondering if this is contam or what, i want to salvage the strain since i used the whole syringe for all the cultures....which were all 1 tablespoon per cup water...what im hoping to do is transfer them to a diff batch i just made (2 teaspoons/quart water)....these are cooling and have no carmalization in em...i just dont want to reorder a another syringe...will the myc still grow in the stronger/carmalized mix? or am what i seeing contam?
You used a whole syringe to make LCs?
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bare.whiterabbit
Lapin Blanc



Registered: 11/29/09
Posts: 912
Loc: The Microwave
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Try transferring to the less sugar'd batch and see if it takes off...if not, I'd even just PC some water to sterility and transfer to it...enough sugar with probably come with to support it
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hossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
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i made 1 pf cake and 6 LCs( 3 honey, 3 light malt)....they look like the puffy little white myc balls...just with a buncha particles stuck all throughout it....im just worried cause ive had a ton of trouble with trich in the past...what does that look like in a LC?
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trust the fungus
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hossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
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Re: LC carmalization [Re: hossfield]
#12169404 - 03/09/10 09:15 AM (13 years, 11 months ago) |
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anyone know will trich look like mycellium in a liquid culture?any have pics?
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trust the fungus
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kiyote
Apprentice Grower



Registered: 07/08/08
Posts: 366
Loc: Near Chicago
Last seen: 11 years, 2 months
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Re: LC carmalization [Re: hossfield]
#12169613 - 03/09/10 10:06 AM (13 years, 11 months ago) |
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The major problem with LCs is that you don't know if they're contaminated until you knock up a bunch of jars and they all get infected. It's the risk you take with this method of making them 
There is another LC method where you take a quart jar of grain, let it get fully colonized and then inject water into the mix, and suck it out with a syringe. That way you can see if there are contams before turning it into a liquid culture.
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andymc
cocoa beetles from zanzibar



Registered: 11/25/09
Posts: 2,395
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Re: LC carmalization [Re: hossfield]
#12170122 - 03/09/10 11:42 AM (13 years, 11 months ago) |
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A few of my LCs have the little puffballs as well. I'd toss them but it looks suspiciously like healthy mycelium, and there are no other obvious signs of contam. I know people say you just have to try it (I inocced with one of them so we'll see what happens) but I'd be curious to know if anyone else has had success with an LC that had the puffballs.
-------------------- How I make spore prints Trade List My flow hood If he asks me "Did you have a good time?" I'll say, "Get the lights, Mr. Grim Reaper" -odds
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myceleus_rex
seeker



Registered: 07/01/09
Posts: 1,581
Loc: alized
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Re: LC carmalization [Re: kiyote]
#12171041 - 03/09/10 02:01 PM (13 years, 11 months ago) |
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Quote:
kiyote said: The major problem with LCs is that you don't know if they're contaminated until you knock up a bunch of jars and they all get infected. It's the risk you take with this method of making them 
There is another LC method where you take a quart jar of grain, let it get fully colonized and then inject water into the mix, and suck it out with a syringe. That way you can see if there are contams before turning it into a liquid culture.
That technique is linked in mi sig
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hossfield
Goomba
Registered: 08/26/07
Posts: 46
Last seen: 12 years, 1 month
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hmmm i wonder if i could do that tech with flour/verm mix? i did do one flour/verm cake that directly got the spores...
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trust the fungus
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andymc
cocoa beetles from zanzibar



Registered: 11/25/09
Posts: 2,395
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Re: LC carmalization [Re: andymc]
#12194901 - 03/13/10 12:28 PM (13 years, 11 months ago) |
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Quote:
andymc said: A few of my LCs have the little puffballs as well. I'd toss them but it looks suspiciously like healthy mycelium, and there are no other obvious signs of contam. I know people say you just have to try it (I inocced with one of them so we'll see what happens) but I'd be curious to know if anyone else has had success with an LC that had the puffballs.
I'll answer my own question, for the benefit of others. Puffballs do not seem to indicate a failed LC. I guess sometimes the myc just does that. I inocculated a couple of jars from LCs that have puffballs, and the jars appear to be colonizing away.
So there we go.
-------------------- How I make spore prints Trade List My flow hood If he asks me "Did you have a good time?" I'll say, "Get the lights, Mr. Grim Reaper" -odds
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DinGusFunGus
Stranger

Registered: 11/21/22
Posts: 1
Last seen: 1 year, 2 months
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Re: LC carmalization [Re: andymc]
#28063142 - 11/21/22 11:48 AM (1 year, 2 months ago) |
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Hey man each of those floating fuzzy balls was one pair of Spores. This happens when making LCs from mss. Anyway you probably know by now whether it was good or bad.
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,317
Last seen: 3 hours, 16 minutes
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Quote:
DinGusFunGus said: Hey man each of those floating fuzzy balls was one pair of Spores. This happens when making LCs from mss. Anyway you probably know by now whether it was good or bad.
Just woke up from a nap and the first thread is a 12 year old bump. Good looks bro!
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: LC carmalization [Re: Screwup] 1
#28063168 - 11/21/22 11:55 AM (1 year, 2 months ago) |
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We can all remain safe and sleep well at night knowing screwup is surely always on the case
-------------------- Being pissed on does not make you a real man.
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