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Unicorncaca
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Registered: 03/02/22
Posts: 34
Last seen: 11 months, 12 days
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Rye grass seed contam
#28062473 - 11/20/22 10:05 PM (1 year, 2 months ago) |
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I followed a violet's tek to a T and had so much contam. I'm just curious if others have had real problems with rye grass seed. Thanks!
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CreonAntigone
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Registered: 05/30/21
Posts: 2,875
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Did you test your culture on other grains for better results? If you're getting a contam over and over it may be the culture and not the grain, especially if the grain has been properly sterilized.
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Unicorncaca
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Registered: 03/02/22
Posts: 34
Last seen: 11 months, 12 days
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I'm it the process of doing that, should know in a week or so. I've never had issues like this, it's quite frustrating.
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CreonAntigone
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Registered: 05/30/21
Posts: 2,875
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Quote:
Unicorncaca said: I'm it the process of doing that, should know in a week or so. I've never had issues like this, it's quite frustrating.
Did you change the inoculum? What did you use - LC? Agar wedge?
I'm trying to judge if the contaminant may have come from the culture, vs the grains.
If nothing changes from before suspect the grains. If you are using a new culture, maybe suspect the culture.
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Unicorncaca
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Registered: 03/02/22
Posts: 34
Last seen: 11 months, 12 days
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I've been using agar and doing so with little issues. So I just lost nine jars with different spawn, I'm baffled I can't figure this out... So I'm probably at about a 50percent success rate.
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CreonAntigone
Stranger

Registered: 05/30/21
Posts: 2,875
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Quote:
Unicorncaca said: I've been using agar and doing so with little issues. So I just lost nine jars with different spawn, I'm baffled I can't figure this out... So I'm probably at about a 50percent success rate.
Pictures would help to determine the exact nature of the contamination, which would suggest solutions.
By the way, you happened to accidentally post in the microdosing forums. You'll have more engagement on the mush cult forum for sure.
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Unicorncaca
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Registered: 03/02/22
Posts: 34
Last seen: 11 months, 12 days
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Haha thanks didn't realize
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