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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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Offlinebent
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Registered: 07/31/03
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Last seen: 11 years, 27 days
Liquid Mycelium Syringe (is this the right tek?)
    #2805334 - 06/18/04 01:51 PM (13 years, 5 months ago)

Taking ~1/4 Pint of Colonized WBS and using a Pressure Box, carefuly place into a pint or quart jar filled with sterilized, cooled water by removing lid and dropping mycelium in. This will all be in the sterile pressure box. Than sealing the jar with the 1/4 pint mycelium to 1 pint water shake and fill brand new unopened sterile syringes through the lid.

Should I add anything to the water such as honey, does tap water or distilled water make a difference? After filling the mycelium syringes should I inject immediately or should I incubate them for a bit to mass out the mycelium. (I once seen have mycelium syringes in a picture of their incubator)

Would it be alright to heat the syringes over an alcohol flame and insert into mycelium water and suck up water while hot? Would it cool down fast enough and allow viable mycelium into the syringe or would it kill it?

What I'm also unsure of is what should I do to the lid. Should I use tyvek over a hole, polyfil, nothing, an injection port, do I need a plastic lid?

Help me out with all questions on all corners because I've learned that its the tiniest things that ruin everything. Thanks so much!


Edited by bent (06/18/04 01:54 PM)


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OfflineAnnoA
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Re: Liquid Mycelium Syringe (is this the right tek?) [Re: bent]
    #2805522 - 06/18/04 02:35 PM (13 years, 5 months ago)

Sounds ok.

>Should I add anything to the water such as honey, does tap water or distilled
>water make a difference?

I would use potable water.


>After filling the mycelium syringes should I inject immediately or should I incubate You can use them immediately.

>Would it be alright to heat the syringes over an alcohol flame and insert into
>mycelium water and suck up water while hot?
Yes.

> Would it cool down fast enough and allow viable mycelium into the syringe
Yes.

>What I'm also unsure of is what should I do to the lid. Should I use tyvek over a
>hole, polyfil, nothing, an injection port, do I need a plastic lid?

A hole for the needle.


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Offlinebent
bent1

Registered: 07/31/03
Posts: 204
Loc: ny
Last seen: 11 years, 27 days
Re: Liquid Mycelium Syringe (is this the right tek?) [Re: Anno]
    #2816762 - 06/22/04 12:19 PM (13 years, 5 months ago)

should i put something in the jar with the mycelium like some pennies or some ball bearings, something to knock the mycelium around?


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Offlinekilgore_trout
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Registered: 10/18/03
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Re: Liquid Mycelium Syringe (is this the right tek?) [Re: bent]
    #2816789 - 06/22/04 12:35 PM (13 years, 5 months ago)

broken glass works well


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Amazon Shop: ½ Pint Jars, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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