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OfflineShroomboofer
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NEW WORLD RECORD: Most Potent Mushrooms Ever Tested * 19
    #28044749 - 11/11/22 04:05 AM (2 years, 2 months ago)

Some of you may be aware of Gordo, he posted on youtube and his Patreon page where he has various mushroom growing teks, psychedelic botanical extraction teks, trip playlists, etc available for free.  Earlier this week, he announced he verified a wild procured a Panaeolus cyanescens variety referred to as the Tamarind Tree British Virgin Islands (BVI) variety to be more potent than any other mushroom publicly known to date.  Other species/varieties with their own unique qualities were also analyzed.  Below is a copy/paste of his post.  Original can be found here.  All his content and spore prints are available for free, but if you so wish, you're welcome to subscribe to help support the work he is doing if you feel so inclined.  Without further to do, the most potent mushroom to date...

NEW WORLD RECORD: Most Potent Mushrooms Ever Tested



I don't think I ever shared this publicly, but I have been on a multiyear quest to find the most potent mushroom on Earth.  There isn't really any grand purpose for this quest, I just thought it would be a fun little contribution to the mycology world and it's exciting to me.  I had previously mentioned that the official world record based on all available data I could find (searching published papers and online sources) was set way back in 2009, it was from pan cyans seized by German customs.  I was surprised that in all the years since then, no one has ever formally described  a more potent mushroom.  I even contacted the author of that paper (Dr. Laussmann) and he confirmed that he has never come across mushrooms that potent since that time.

So I scoured the globe sending inquiries to various collectors around the world and I connected with many wonderful mycologists and hobbyists who sent amazing prints from distant lands (France, Switzerland, Japan, USA).  Based on anecdotes alone from those that sent spores, I focused on growing out the 4 cultivars that seemed most promising.  None of these cultivars had ever been HPLC lab tested as far as I know.  For growing I used the same pan cyan TEK previously described  with both coir and straw substrates (to compare differences) but I moved it into a mini greenhouse and decreased humidification time to just 20 minute intervals every 3 hours, a small heater on a thermostatic switch was used to keep it above 70F (21C) at all times, and I used a small computer fan on a timer to blow in fresh air every hour for 30 minutes (12 hours of forced fresh air daily). I also sprayed the casing with water manually as needed each morning and evening to ensure it was always moist.  This setup worked perfectly for me.

The four cultivars I focused on were Panaeolus cyanescens: Tamarind Tree British Virgin Islands (BVI):

These came from a land where psychedelic mushrooms are celebrated, legal to possess and consume.  Maybe the destination for our next GordoTEK live trip event?  Credit goes to V.L for the spores (he is a legend and maybe the most prolific spore trader I know of).  These spores originally came to V.L from a Virgin Islands native resident who discovered them in the wild (that person actually still sells spores, for microscopy only, under the name TamarindTreeBVI).  One of the original prints was as large as 4-5cm across but the others were more typical 1-2cm.  V.L has been working these genetics for a couple of years.

Pan Cyan: Purple Haustralia Venom (PHV):

A venom hybrid from Jake Oncid that comes from a cross between the pan cyans of the tropical region of the Gulf of Mexico known as Hausteca with pan cyans from Australia.

Pan tropicalis Australia venom cross with pan cyan Jalisco Mexico: Nec-divino the wizard (Nec'D) developed by Jake Oncid.

Finally, an unnamed cultivar of Panaeolus bisporus provided by V.L who acquired it in a trade, grew it indoors then spread it in his greenhouse where they naturalized and now come back every summer (this has been going for at least 3 years).

I generally like to avoid tainting people's expectations by sharing anecdotes but what I will say about bisporus is that once again the consensus view is that this species is "different" and very positive, that's about all I will say, if you want to dig deeper you can. But worth noting that bisporus tested HIGHEST in norbaeocystin which could be an important factor with regards to user experience, we don't know much about norbaeocystin yet.  I can tell you that bisporus grows very slowly compared to the other 3 cultivars, BVI grew the fastest, matching the speeds and aggressiveness of Estero while bisporus took nearly double the time to go from spores to pins (but once bisporus starts fruiting it just seems to keep producing flush after flush for a very long time).  All four cultivars worked great with my TEK.  They were all very easy to grow and none seemed prone to contamination.  BVI was not only the most aggressive of the 4 but it also produced the biggest yields and carpets of fruit bodies and produced wave after wave of fruits.  It also produced the most delicate fruit bodies, if you blow on them you can knock them over.  In mushroom folklore many believe more delicate smaller mushrooms produce more gentle and positive, euphoric experiences.  When dehydrated BVI became almost "weightless":

This cultivar seems to produce less mushroom fiber/bulk which may have benefit to those that consume it (such as less nausea).  But it also has the effect of improving the alkaloid to weight ratio.  In other words, a single flush of Estero and BVI in the same size growing tray will likely produce about the same grand total of alkaloids, but the dry Estero will weigh twice as much because its fruit bodies are thicker and meatier, so the potency or % active alkaloids per unit of weight are twice as high in the BVI cultivar.  This resulted in the world record for most potent mushroom with Psilocybin equivalent alkaloids at 7.06% (note that psilocybin equivalent means adding the measured psilocybin to the psilocin*[molecular weight of psilocybin/molecular weight of psilocin] this is to account of the fact that psilocin is approximately 39% more potent by weight than psilocybin.  This is also the procedure that will be used in labeling of psilocybin products sold in Oregon under their legal access plan).

You can find the 160 page lab report (or 22 page summary report) here. All four of my samples tested very potent as can be seen in the first picture in this post but BVI was extraordinary.  The HPLC testing for this project was provided by Doma "MagicMyco" Nunzio. 

I want to say a few things about MagicMyco... his is the ONLY lab being radically transparent and totally "open sourced".  He has documented his entire testing procedure in minute detail for anyone to scrutinize and/or criticize.  This is how science SHOULD WORK, the other labs mostly want to hide and keep things proprietary and we have no real idea what they are doing or if their methods are even reliable.  Myco has dedicated countless hours to studying/comparing extraction techniques and solvent systems and endless tweaking of his TWO Agilent HPLC machines, he has read books on the art of HPLC and has been personally mentored by Felix Blei of Miraculix.  He uses fresh standards before each batch of testing he conducts.  Myco has an incredible lab and he even invited me over there several times, I think I've spent over 50 hours in his lab at this point!  He is doing important mycology research and we are discovering new things every month (for example how do different substrate ingredients impact alkaloid levels? how do different drying temperatures and techniques impact alkaloids? what ARE all of the alkaloids in each cultivar? does freeze drying help or hurt alkaloid content? how quickly do psilocybin and psilocin degrade over time? what is the best way to store mushrooms? etc).  It is my hope that other labs follow Myco's lead and start publishing useful research and sharing their methods.  Iron sharpens iron.  My point in saying all of this is that I think MagicMyco deserves our support, and the ways you can do that are to follow him on social media, visit his website (https://magicmyco.org/ ) use his testing service, read his monthly research reports, AND PARTICIPATE IN HIS CULTIVAR CUP!  He even created a coupon code "GordoTEK" that gives us a discount on Cultivar Cup entries. This is NOT an affiliate marketing thing, I get NO compensation from Myco whatsoever, I asked HIM to create the coupon for you guys and I am promoting him here because of all the reasons mentioned above.  I want him to survive so he can keep supporting the psychedelic community and for selfish reasons too, so I can keep going back to his lab as needed to get the science I'm interested in done, hahah).

Final thoughts: I realize that the BVI potency HPLC test numbers are so high they will undoubtedly be questioned.  They were questioned by me, only to find out that Doma himself did not believe the results at first and he delayed publishing the report for several days as a result while he tested the sample over and over again to make sure it was accurate.  Since these results are extraordinary (the potency is 49% higher than the previous world record) they really do need confirmation from other labs.  I am sure that will happen in coming months.  I should also point out that I actually harvested and dried the samples ON THE SAME DAY that I brought it to the lab for testing, it doesn't get any fresher than that! Also note that the fruit bodies were harvested in a slightly immature stage of development as can be seen in the pictures and this boosts alkaloid to dry weight % as well (I'm giving away all of my secrets).  Psilocin deteriorates pretty quickly, so I think these results could be difficult to reproduce unless the samples were just as fresh and harvested/handled the same way.  Even if the same sample were to be tested a few weeks later it could be substantially lower in potency.  The same can be said for anyone growing this cultivar, if you store it for months before consuming it, the potency will undoubtedly be significantly lower.  I will post about alkaloid deterioration, best storage practices, and other subjects in future posts but for right now I just want to say that oxidation is the biggest problem, eliminate the oxygen and you will have the best results.  Vacuum sealing is one option but I prefer to store in glass jars and vacuum sealing jars is unreliable, the very simple solution is to fill the jar with a heavier than air inert gas.  I used to use CO2 for this, but now they sell inexpensive small bottles of Argon on Amazon that work beautifully to completely protect your mushrooms from all oxidation and a single small bottle is enough for 75 mushroom jars!

p.s. I know you guys will want spores, and YOU SHALL HAVE THEM, but give me some time, I need a month at least.  Demand will probably be very high, so I'm only giving these to patrons for now.  If you can't wait for the free prints from me, you can always track down vendors selling these spores or go directly to the original source in the British Virgin Islands (TamarindTreeBVI@protonmail.com) although I cannot guarantee the prints he is selling today are the same genetics that set the world record.
______________________________________

Links to all of my TEKs, Videos, Trip music, and frequently asked questions with answers can be found here: https://www.patreon.com/posts/22774475 (everything is made available for FREE, I do not use pay walls, support the work only if you feel like you've benefited from it and you are able to do so).

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OfflineExbat
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shroomboofer]
    #28045795 - 11/11/22 05:03 PM (2 years, 2 months ago)

Wow, thanks for sharing your in-depth research. Very interesting reading.

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Offlinebongoman
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Exbat]
    #28046232 - 11/11/22 10:19 PM (2 years, 2 months ago)

Awesome thread!

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InvisibleMariaTruthSeeker
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shroomboofer] * 1
    #28047221 - 11/12/22 03:09 PM (2 years, 2 months ago)

Its really cool to see this research being done, we are still in the "early days" of mushroom scientific analysis, there is so much yet to be learned.  That record is pretty insane too, I would think even a 1 gram dose would be very intense, maybe overwhelming (assuming it was taken right after harvest and drying).

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OfflineShroomboofer
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: MariaTruthSeeker]
    #28064209 - 11/22/22 12:10 AM (2 years, 1 month ago)

Quote:

MariaTruthSeeker said:
Its really cool to see this research being done, we are still in the "early days" of mushroom scientific analysis, there is so much yet to be learned.  That record is pretty insane too, I would think even a 1 gram dose would be very intense, maybe overwhelming (assuming it was taken right after harvest and drying).



Speaking of the early days, have you seen Hamilton's last episode on Vice titled Ultra LSD?  In it, he interviews a professor who identified and modeled the precise structure of LSD's molecular target neurologically, and this will pave the way to thousands of new compounds.

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OfflineDreamEasy1111
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shroomboofer]
    #28076186 - 11/30/22 02:03 PM (2 years, 1 month ago)

this is absolutely incredible information


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im new go easy on me! love to learn and ill ask a lot of questions!

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OfflineHindsight
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: DreamEasy1111] * 5
    #28076245 - 11/30/22 02:32 PM (2 years, 1 month ago)

I've grown BVI and can confirm they are very potent. I feel semps are stronger though - would like to see them tested and compared.




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InvisibleShrimps
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Hindsight]
    #28076256 - 11/30/22 02:36 PM (2 years, 1 month ago)

Is there a special reason you grow in those trays? Wouldn't it be easier just to use like glass that you can wash and reuse? I could imagine those tin trays could cost a bit something over the years ^^

Damn those BVI look great man! Hope to get soon some pictures of my own to show :laugh:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
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Recognizing and dealing with contamination

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Invisibleruawakeyet
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shrimps] * 2
    #28076280 - 11/30/22 02:45 PM (2 years, 1 month ago)

I started with glass baking dishes that were about 2 inches deep. I found that they slid around on my fruiting table a lot, and I had to be super careful with them to keep from chipping the edge or worse, breaking one all together. They also didn't come with lids, so I would use foil or cling wrap to cover them during colonization. Initially I liked the idea of being able to use heat to sanitize my trays after a trich outbreak, but I think a good washing and drying should take care of any leftover mold spores.

Now that I'm looking to increase my sub depth to around 3 inches, I am switching to plastic food storage containers. (~ 3 inches deep) They come with a lid so I won't have to fool with foil or cling wrap. They shouldn't be as slick on my fruiting table and should stay in place better. Of course, I don't have to worry about them chipping and they are all but unbreakable. My plan is to drill some small holes in the lids for air exchange during colonization, then case and place them into fruiting.

I like containers that let me bring the top of the substrate up to the top of the container for easier harvesting, and better airflow.

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OfflineDreamEasy1111
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: ruawakeyet] * 1
    #28076296 - 11/30/22 02:53 PM (2 years, 1 month ago)

Quote:

ruawakeyet said:
I started with glass baking dishes that were about 2 inches deep. I found that they slid around on my fruiting table a lot, and I had to be super careful with them to keep from chipping the edge or worse, breaking one all together. They also didn't come with lids, so I would use foil or cling wrap to cover them during colonization. Initially I liked the idea of being able to use heat to sanitize my trays after a trich outbreak, but I think a good washing and drying should take care of any leftover mold spores.

Now that I'm looking to increase my sub depth to around 3 inches, I am switching to plastic food storage containers. (~ 3 inches deep) They come with a lid so I won't have to fool with foil or cling wrap. They shouldn't be as slick on my fruiting table and should stay in place better. Of course, I don't have to worry about them chipping and they are all but unbreakable. My plan is to drill some small holes in the lids for air exchange during colonization, then case and place them into fruiting.

I like containers that let me bring the top of the substrate up to the top of the container for easier harvesting, and better airflow.




currently using plastic food containers like you mentioned and love them. I found stacking them helps with condensation on the lids, but definitely drill holes. I drilled 6 on the outer edges so i could stack them and then one in the center for the hell of it. Worked out well but i think i needed to make the holes bigger. Ill report back on the next run.


--------------------
im new go easy on me! love to learn and ill ask a lot of questions!

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InvisibleShrimps
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Registered: 10/13/22
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: ruawakeyet]
    #28076297 - 11/30/22 02:54 PM (2 years, 1 month ago)

Good point! I was also thinking doing my next pan's in a try (plastic without lid which I have laying around), and filling the floor of the mono with Water and some Seramis and placing the try on top. I used to grow cubes that way and I think it would suit the pan's better with higher RH.

Also harvesting is much easier than in a mono^^

(you all grow in martha's as it seems, would a JCM box bi a bigger improvement than just like a watertub?)


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

Edited by Shrimps (11/30/22 02:55 PM)

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Invisibleruawakeyet
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shrimps] * 1
    #28076319 - 11/30/22 03:02 PM (2 years, 1 month ago)

I think a JCM FC would be better for pans, due to the FAE provided by the air pump.

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Invisiblefungusul
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: ruawakeyet] * 1
    #28076820 - 11/30/22 07:01 PM (2 years, 1 month ago)

Thanks for sharing this information! :mushroom2:

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OfflineHindsight
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shrimps] * 2
    #28076857 - 11/30/22 07:14 PM (2 years, 1 month ago)

Quote:

Shrimps said:
Is there a special reason you grow in those trays? Wouldn't it be easier just to use like glass that you can wash and reuse? I could imagine those tin trays could cost a bit something over the years ^^

Damn those BVI look great man! Hope to get soon some pictures of my own to show :laugh:




I grew the BVI in those pics almost a year ago. Back then I was using thin substrate depths and didn't want high walls around the sides that would prevent high amounts of air circulation so I wanted a tray with the same depth as my target substrate depth. Since then, I have gone thicker with the substrates and now definitely prefer it. I now grow all my pans and semps in my tent in 6qt purple latch sterilite shoeboxes (like the ones shown in most of the pics in my signature). And of course, the sterilite shoeboxes get re-used.

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OfflineSingularFusion
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shroomboofer]
    #28077010 - 11/30/22 08:03 PM (2 years, 1 month ago)

Very interesting, thanks for sharing

Saw another round of testing that was done on the GordoTEK Nec-D where it also achieved above 7% total alkaloids, can't find the link now. I didn't realise BVI were also hitting these numbers

sounds impossible to my cube brain but I am sure it isn't

these things must be extraordinary to enjoy :cheers:

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OfflineDreamEasy1111
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: SingularFusion] * 2
    #28077966 - 12/01/22 12:15 PM (2 years, 1 month ago)

i experienced 0.71 g of a Pan Cambo strain not long ago..... i felt like i overdosed... and yes they were dry and about a month old. I cant imagine trying a BVI or Nec D that came from that test... wooof


--------------------
im new go easy on me! love to learn and ill ask a lot of questions!

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InvisibleShrimps
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Hindsight]
    #28078007 - 12/01/22 12:39 PM (2 years, 1 month ago)

Quote:

Hindsight said:
Quote:

Shrimps said:
Is there a special reason you grow in those trays? Wouldn't it be easier just to use like glass that you can wash and reuse? I could imagine those tin trays could cost a bit something over the years ^^

Damn those BVI look great man! Hope to get soon some pictures of my own to show :laugh:




I grew the BVI in those pics almost a year ago. Back then I was using thin substrate depths and didn't want high walls around the sides that would prevent high amounts of air circulation so I wanted a tray with the same depth as my target substrate depth. Since then, I have gone thicker with the substrates and now definitely prefer it. I now grow all my pans and semps in my tent in 6qt purple latch sterilite shoeboxes (like the ones shown in most of the pics in my signature). And of course, the sterilite shoeboxes get re-used.




Aah okay, do you also close the lids of the shoeboxes douring colonialization? Or just hit them right up into fruiting after casing?


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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OfflineHindsight
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shrimps] * 1
    #28078130 - 12/01/22 01:37 PM (2 years, 1 month ago)

Quote:

Shrimps said:
Aah okay, do you also close the lids of the shoeboxes douring colonialization? Or just hit them right up into fruiting after casing?




I close the lids and have them outside of the tent, but in 78F temps while they colonize. Then I wait a few more days for consolidation, then I remove the lid, case them, and put them in the tent without the lid.

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InvisibleMariaTruthSeeker
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Hindsight] * 4
    #28087459 - 12/07/22 02:41 PM (2 years, 1 month ago)


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Invisibleruawakeyet
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: MariaTruthSeeker]
    #28087480 - 12/07/22 03:02 PM (2 years, 1 month ago)

Quote:

MariaTruthSeeker said:





I LOVE this!!!! Please post this in the Official Pan Thread! Before I do! :smile: (JK, I'd still quote you.)

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Offlinepitriot
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: Shroomboofer] * 1
    #28112447 - 12/25/22 09:15 PM (2 years, 22 days ago)

Thanks for your thoughtful and detailed post.
Also thanks for the link to the Aragon. How would you fill say a mason jar with this spray and close the jar?

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OfflineMysticLullaby
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: pitriot] * 1
    #28112483 - 12/25/22 10:36 PM (2 years, 22 days ago)

educate the noobs that pan cyan IS the best!

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Offlinejohnukguy
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Re: NEW WORLD RECORD: Most Potent Mushrooms Ever Tested [Re: MariaTruthSeeker]
    #28185004 - 02/13/23 05:37 AM (1 year, 10 months ago)

Well that wins the internets for today. :smile:


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* not very potent
( 1 2 all )
cheerios 5,033 36 12/06/02 09:30 AM
by dankcrop
* Potency which strain do you think is the most potent ? Anonymous 1,227 15 03/17/03 07:49 AM
by boomer420
* Re: Most Potent Shrooms!? Code 1,592 6 01/22/00 09:10 PM
by Condi1
* most easiest to grow and most potent blokar 10,773 5 08/16/01 07:54 PM
by Zen Peddler

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