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Offlinerifters360
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Registered: 11/04/17
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possible alternative to taking spore prints
    #28029169 - 11/02/22 09:19 AM (2 years, 2 months ago)

Taking spore prints while necessary to keep varieties, is hard to get them really clean IME. And the reason, just haven't found a way to do them, i think will be clean enough for my expectations, well at least one thats simple enough for me. so i began to think of a solution and set up this experiment. My experiment involves taking a live fruit, of a variety that has a more pronounced veil and an individual fruit that has both dropped spores on the veil and very recently or has started to have the veil tear away. collect the vail remnants carefully with a sterile instrument and put them in something sterile so it can totally dry.  that part already is already done, with two varieties Colombian Rust Spore and a GT variety, and already put them to agar. The Colombian and the Gt both showed really nice growth upon germination and that growth was transferred. However the transfers taken from the Colombians, do not have the growth that was targeted, some very wispy growth and thats likely an error. But the GTs are looking great. the Colombians are on T6 and the GTs are on T4. the GTs are clean and ready and to go the Colombians like I said not so much. So I'm wondering do u guys think, was a fruit just cloned or did the spores germinate or what? interested in y'alls opinions.


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Offlineschpat
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Re: possible alternative to taking spore prints [Re: rifters360] * 2
    #28029177 - 11/02/22 09:25 AM (2 years, 2 months ago)

I suppose the veil tissue is myc so you may just have cloned that.  But my question is why?

If you want to store a culture for a long time there are teks for that.  And if you are pheno hunting then cleaning a germ plate on agar is not very hard.


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OfflineSirPsycho
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Re: possible alternative to taking spore prints [Re: schpat] * 1
    #28029201 - 11/02/22 09:49 AM (2 years, 2 months ago)

You were on the right track but lost it. Just print from fruit with unbroken veils. Remove the veil with a sterile instrument in an SAB or in laminar flow, cut stem above veil remanent and print the cap.


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OfflineLand TroutM
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Re: possible alternative to taking spore prints [Re: SirPsycho] * 2
    #28029253 - 11/02/22 10:36 AM (2 years, 2 months ago)

Funny cause I’m always surprised by how clean spore prints can be.  I’ve taken lots of prints from outdoors that have matured with wind and rain, even have springtails or worms on them and figure it could take some cleaning up, but will end up with clean enough germ plates to put to grain.  If all your doing is taking spores to agar you can also just take your fruit and streak gills with a flamed loop and put it righty on agar. 
One of the things about spore prints is that they’re easy to catalogue and mail.  No reason to take them just to get another culture.

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OfflineLogicaL ChaosM
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Re: possible alternative to taking spore prints [Re: rifters360] * 2
    #28029395 - 11/02/22 01:02 PM (2 years, 2 months ago)

I use Eatualive's spore printing Tek pretty closely and I get some clean prints from it: https://www.shroomery.org/forums/showflat.php/Number/24732724#24732724

I make some pretty clean spore syringes this way :thumbup:


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Offlinebongoman
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Re: possible alternative to taking spore prints [Re: rifters360]
    #28029733 - 11/02/22 04:51 PM (2 years, 2 months ago)

In vitro grows can yield virtually axenic spore prints.

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Offlinerifters360
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Re: possible alternative to taking spore prints [Re: SirPsycho]
    #28030760 - 11/03/22 07:18 AM (2 years, 2 months ago)

because it can be done all at once. but you think even if the veil remnants are dry that the spores on it may germinate? like both revive the dry tissue and spores germinate? but also im not a master cultivator or anything i just need more experience. i do take prints all the time and they are usually clean but i just have been having some difficulties that made me not want to bring fruits into my flowhood/clean space. my last grow of 4 tubs 2 had bacterial stuff going on. i did six this time and quite fucked up by not having any gauges or anything and not knowing co2 was also a problem. after that began developing primordia they just stopped and then the bacteria problem came back when i used a spray bottle on 2 of the six tubs that had been sitting for two months with out being cleaned from the grow before. so im gonna deep clean my fruiting space ehich is just a ten with the flaps all the way open. the tent is only so my cats dont mess with anything. i got a lot to learn is the answer to why


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Let It Be...

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OfflineDredgeMyEyes
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Re: possible alternative to taking spore prints [Re: rifters360]
    #28030773 - 11/03/22 07:42 AM (2 years, 2 months ago)

I usually just let a no-pour screw top plate pin, fruit and sporulate so I can use that as a clean print essentially. I turn it on it's side so some spores land on the plastic instead of all on the agar

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OfflineLand TroutM
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Re: possible alternative to taking spore prints [Re: rifters360]
    #28030779 - 11/03/22 07:53 AM (2 years, 2 months ago)

That sounds a lot more like a spawn issue than a spore issue.

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Offlineschpat
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Re: possible alternative to taking spore prints [Re: Land Trout]
    #28030834 - 11/03/22 08:53 AM (2 years, 2 months ago)

Yeah man, it sounds like you need to get the basics right.  Post your process with pics for each stage and we will do our best to help you.


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OfflineLand TroutM
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Re: possible alternative to taking spore prints [Re: schpat]
    #28032471 - 11/04/22 10:43 AM (2 years, 2 months ago)

And riffters, there’s nothing really wrong with the way your collecting genetics there.  If it works for you than it works, the bottom line is what’s important.  But like said, you might just have clones growing from the tissue before the spores germinate which, that’s not a real problem, just not be quite the same as another true generation.

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