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Tormato  
Chairman of the Bored




Registered: 07/01/17
Posts: 6,106
Last seen: 2 days, 11 hours
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
#28026796 - 10/31/22 07:28 PM (2 years, 2 months ago) |
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Quote:
Envypenis said: Awesome thank you that was what I pretty much got when I did look into it. For bacterial cultures you would flip, not for mycology. Definitely helps ease the mind
I have and never will flip a plate upside down for any reason, especially if it's got condensation on the lid. All yours gonna do is flip it and dump the water onto the plate.
Just be careful when you move them around and removing the lid when you use em.
There's ways to get rid of it, but I'm lazy as fuck and I just don't worry about it. 
I feel the condensation helps keep the plate fresh longer. If there's condensation they can't be dried out, right? 
That's just how I get down.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 8 months, 22 days
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings]
#28026866 - 10/31/22 08:15 PM (2 years, 2 months ago) |
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Quote:
bakedbeings said: idk bro thats why i stick to no-pours
regarding sterile technique just imagine that any unsterile surface is covered in contam that can transfer by contact, any air that isnt laminar flow or completely still is full of contam that wants to land in your precious container, and any unsterile object you wave around can shed contam either down (SAB) or towards you (LFH)
you are playing goalie for a sterile food source, against invisible balls
My balls are visible. Wanna see?
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
We don't own things - things own us.
Semi-solid liquid culture (SSLC)
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SymPlayTon
Noobinstien

Registered: 09/03/22
Posts: 420
Loc: In a state of confusion
Last seen: 1 year, 3 months
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
#28026884 - 10/31/22 08:22 PM (2 years, 2 months ago) |
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I use wash cloths and binder clips. Washcloths lightly damp with soapy water or bleach water clipped to the top of the arm hole and let to drape down.
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SingularFusion


Registered: 10/31/18
Posts: 1,303
Last seen: 1 month, 2 days
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Re: 70% iso doesn’t kill all bacteria [Re: SymPlayTon] 1
#28027106 - 10/31/22 10:30 PM (2 years, 2 months ago) |
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Nice changes Envy
sure that new SAB is gonna serve you well
all the best with it going forward
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Envypenis
Stranger

Registered: 10/29/22
Posts: 24
Last seen: 2 years, 1 month
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Re: 70% iso doesn’t kill all bacteria [Re: Tormato]
#28027546 - 11/01/22 08:36 AM (2 years, 2 months ago) |
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I can’t believe how much I overcomplicated this whole deal. One less waste of time lol I had noticed If I had condensated cups and older agars that had dried out a bit.. take the drier wedge to the wetter agar and would sometimes come back for me. Are there any other tricks for trying to save dried agar samples? The last few batches of agar I effed up royally I really needed those genetics to continue on. Most have dried too much by now.
Preliminary report and probably way too early to tell.. but ALL agar cups from last night using properly adjusted technique looked clean this morning. Have had the tent set at 82* and will hopefully have same results in another 12 hrs
Agar was setting pretty good by the end of pouring and some cups didn’t set entirely flat. Some maybe a little bumpy on the surface. Only thing I couldn’t tell what it was.. could see some shiny spots but no cloudiness what so ever. Not sure if that was condensate that fell and got absorbed?
Edited by Envypenis (11/01/22 08:44 AM)
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TheUsualSuspect
never tell me the odds


Registered: 09/11/22
Posts: 260
Last seen: 2 years, 5 days
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
#28028712 - 11/01/22 09:31 PM (2 years, 2 months ago) |
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Quote:
Envypenis said: I can’t believe how much I overcomplicated this whole deal.
I assure you, everyone does that. My first grow I did spores to grain bag and had a nasa looking mini tent with humidifyer and fans and sensors... etc.
Needless to say the second attempt looked completely different.
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