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K.KrazyMyc
Mestizo


Registered: 10/30/22
Posts: 15
Last seen: 2 years, 1 month
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K.Krazy's Monotubs,ShoeBoxes,and everything else.
#28027869 - 11/01/22 02:09 PM (2 years, 2 months ago) |
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Hello my brothers and sisters, my fellow Shroomerites. This will be a semi continual log and discussion about my current and future project, if I decide to keep the future projects here.
I'm an old school forum guy, not just here but cannabis forums. I haven't posted in any in a LONG time, so bare my certain lack of "style" right now...it'll come back in time..
I was a member here back in the days when RR and the rest of the old school cats were around,when R44 was the best dude to get the spores from :/...yes..that long 
So..let's start at the beginning...a year ago I ordered AA+,and Penis Envy spore syringes.. with a Costa Rica freebie and a PF RedSpore freebie. They were stored as such :  in the refrigerator .
Around 8/20/22 I prepared WBS,with regular mouth quart jars,RTV'ed SFD,and RTV injection ports. 
This is my basic WBS prep :
1. Measure .5 qt of WBS for each quart jar intended.
2.Rinse very well in cold and hot tap water, until all floaters removed and water runs clear.
3. Add gypsum.Let soak in hot tap water overnight (12- 16 hours)..no exact time/
4.Bring water to low simmer,As soon as it STARTS to steam off,quickly dump WBS in kitchen strainers. . Toss WBS in strainer so WBS can steam dry proper. . 5. Vent PC for 15 minutes , pressure cook for 90-120 minutes.
6.Optional (beneficial IME, remove jars while hot,a few hours after PC,and shake well and put into SAB.
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K.KrazyMyc
Mestizo


Registered: 10/30/22
Posts: 15
Last seen: 2 years, 1 month
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Re: K.Krazy's Monotubs,ShoeBoxes,and everything else. [Re: K.KrazyMyc]
#28027984 - 11/01/22 03:13 PM (2 years, 2 months ago) |
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This is where I will add my few observations/anecdotes concerning WBS preparation..and even brand.
8/25/22
Inoculated 10 Quart Jars and 6 pint jars with Costa Rica..0.5cc per Quart,.25cc per pint..1 pint control jar
A few jars of PF RedSpore inoculated via spore syringe on 8/28/22
Same for AA+ 8/29/22 .
9/1/22 6 jars of PF RedSpore germinated,4 days after inoculation .
9/4/22
Inoculated 10 quart jars WBS with Penis Envy spore syringe( 1 year old syringe)
New fresh syringes of AA+ and Oak Ridge came.
9/20/22
Inoculated AA+,OakRidge, second syringe of Penis Envy(1 year old syringe).
This is an ongoing project, with procedures from the past few months...time and exact numbers are not precise.
Long story short, the 1 year old A++ and Costa Rica did not germinate, the 2 P.E syringes (also 1 year old) were very viable. These are a few pictures of a vigorous P.E Ms culture..from the original 1 year old syringes.   
I have always used Pennington's WBS..however after what I consider proper prep.. none of my Costa Rica or AA+ syringes germinated. This was most surprising, as the spore syringes were literally a year old, stored as above,.Of course we know they SHOULD last more than a year...sadly not in my case.
Without going into exact dates and variables. I spawned a monotub with the original RedSpore jars...the substrate was too wet past field capaocity..it was nasty and bacterial, and thrown away.,
Edited by K.KrazyMyc (11/01/22 03:22 PM)
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