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OfflineAnonymousPixels
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Registered: 10/07/22
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Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar?
    #28022731 - 10/29/22 11:34 AM (2 years, 2 months ago)

Howdy there, I'm a beginner mushroom hunter and cultivation student.

About two weeks ago I had the great fortune of finding a cluster of active Gymnopilus Luteus mushrooms growing on a rotting hardwood log.

I took a spore print from some caps, and later scraped some spores onto agar on 10/16/22. (14 days ago.)

In one of my cups I noticed a fluffy white growth. I wasn't certain if it was indeed mycelium or cobweb mold. But since I'm still learning about agar work. I decided to transfer the white growth to 4 separate fresh agar cups on 10/24/22.

Today marks 6 days since I transferred that white growth onto fresh agar and it looks neat.

As you can see in the photos below : The growth looks evenly circular all around from the agar wedge it was transferred from.

Cup 1 :





Cup 2 :



Cup 3 :





Cup 4 :



---
Up close the growth does look fluffy, and definitely white. It also appears to adhere closely to the agar substrate.
Now the way it's grown nearly perfectly circular is interesting. I'm not sure if this is a sign that it is indeed mold or if this indicates I somehow miraculously isolated a monokaryon strain of Gymnopilus Luteus.

---

I would also like to show some images of the cups I inoculated with spores on 10/16/22. (14 days ago.)

To compare the activity shown, and perhaps to give a better indication of whether or not I've got mycelium growing or some contam brewing.

In this 1st cup : ~ It looks like some mycelium is just starting to grow in a circular formation where the spores were placed.



In this 2nd cup : ~ Looks like some fluffy wispy growth alongside some obvious contamination.





And in this 3rd and final cup : ~ Some very interesting activity. In my opinion it looks like tomentose mycelium growth alongside some contamination. However this growth doesn't look as wispy or fluffy as the other cups I've shown. Perhaps this is the true Gymnopilus Luteus mycelium growth I've been looking for!


---

That's all I've got to show on this project for now.

I would greatly appreciate your thoughts or any feedback you could give me regarding these photos.
I know agar growth is hard to identify, and pictures can only do so much.

Either way I would enjoy reading anyone's take on these samples since I'm still learning about agar work and how different types of mycelium / contam develops on agar.

Thx :mushroom2: :p

Edited by AnonymousPixels (10/29/22 02:29 PM)

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OfflineRusty2096
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Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: AnonymousPixels] * 1
    #28022776 - 10/29/22 12:16 PM (2 years, 2 months ago)

They all look like mold to me but I'm not familiar with gyms so :shrug:


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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OfflineAnonymousPixels
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Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: Rusty2096]
    #28022926 - 10/29/22 02:27 PM (2 years, 2 months ago)

Well thanks for your feedback anyway.

Are there any physical ways of confirming whether or not a particular growth is mold? (Without a Microscope)

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OfflineRusty2096
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Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: AnonymousPixels] * 1
    #28022941 - 10/29/22 02:39 PM (2 years, 2 months ago)

Quote:

AnonymousPixels said:
Well thanks for your feedback anyway.

Are there any physical ways of confirming whether or not a particular growth is mold? (Without a Microscope)




Gyms cultivators can propable confirm form the pics you posted.

I'd suggest posting em in the contamination forum for better visibility.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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OfflineAnonymousPixels
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Registered: 10/07/22
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Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: Rusty2096]
    #28023118 - 10/29/22 04:14 PM (2 years, 2 months ago)

Heh I was honestly following your suggestion but apparently that forum doesn't exist anymore.

According to LogicaL Chaos.
"As many have likely noticed already, the Contamination Forum is no more. This is because the mods of the Shroomery decided that the Contamination Forum was not getting enough traffic to be viable and it was discontinued as a forum.

Thus, the Contamination Forum has been merged into Mushroom Cultivation. All topics about infections and contaminations will now be discussed here in Mushroom Cultivation.

All Stickied threads from the Contamination Forum are now at the top of Mushroom Cultivation for your reference.

If you want to ask about a contamination issue on your grow, please use the new "Contamination" category option when you create a new thread.

Thank you. That is all.

- L.C."

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OfflineRusty2096
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Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: AnonymousPixels] * 1
    #28023120 - 10/29/22 04:15 PM (2 years, 2 months ago)



--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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OfflineAnonymousPixels
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Registered: 10/07/22
Posts: 18
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Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: Rusty2096] * 1
    #28023133 - 10/29/22 04:24 PM (2 years, 2 months ago)

Ah thank you!

I'll ask over there then. :thumbup:

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OfflineAnonymousPixels
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Registered: 10/07/22
Posts: 18
Last seen: 1 year, 3 months
Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: AnonymousPixels]
    #28024384 - 10/30/22 11:52 AM (2 years, 2 months ago)

I don't blame y'all for being a tad unfamiliar with this particular genus / species.

It certainly ain't no cube.
But it can definitely take you to the same realms.

I'll continue searching for past threads and other resources showing actual Gymnopilus mycelium growth.

If you have any suggestions as to where I could find Gymnopilus mycelium reference images or perhaps know of some Gymnopilus culfivators then please do let me know!

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OfflineRusty2096
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Registered: 08/23/22
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Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: AnonymousPixels] * 1
    #28024746 - 10/30/22 04:28 PM (2 years, 2 months ago)

Try here - > AGAR ENVY! (Anything and All things agar!)

But if I were you, I'd make a very short post explaining what it is with only 2-3 pics max. Many seem to dislike having to review many pics and read a long text when it's not necessary.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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OfflineAnonymousPixels
✰ ⋆ A Young Mage ⋆ ✰
I'm a teapot User Gallery


Registered: 10/07/22
Posts: 18
Last seen: 1 year, 3 months
Re: Agar ID Request : Likely Gymnopilus Luteus Mycelium Growth on Agar? [Re: Rusty2096] * 1
    #28025355 - 10/30/22 10:27 PM (2 years, 2 months ago)

Solid advice. I wasn't sure if people on here wanted super detailed requests or short, sweet, and straight to the point asks.

I'll give that a go then mate. Thx again.

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