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Offlinemake air go wooo
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Registered: 12/02/21
Posts: 60
Last seen: 1 year, 9 months
agar to grain using pins?
    #28024019 - 10/30/22 05:40 AM (2 years, 2 months ago)

Hey there,

    Is there any benefit to letting your agar plates pin, take a clone from that pin, put the clone to fresh agar plates.  So, your now making mycelium growth from a pin.  My question is, is there any benefit to using clone mycelium growth versus just regular mycelium growth, when transfering to grain?

BOD's Comprehensive Agar TEK

Quote:

if you leave a dish about 6-8 weeks usually it will start to knot/pin and then a few more days and you'll have a pin to clone.

Strain Isolation


Many people get overly concerned with finding an isolate. Many isolates suck.
any two spores that successfully mate make a strain.

thus with spores on a petri dish
Quote:

RogerRabbit said:
It's very normal not to see the rhizomorphic growth until you make  few transfers.  With multispore inoculation on agar, you have hundreds if not thousands of strains growing together and all over on top of each other.  Remember, any two spores that germinate and 'pair up' by forming a clamp connection is the very definition of a 'strain'.  Just dig in and take a very small piece of mycelium no larger than a grain of rice.  Move this to a new dish.

When this dish begins to grow out, you may or may not begin to see sectors.  It might take a second transfer before you get few enough strains to see the individual sectors.  Take the best looking growth again and move it to a fresh petri dish.  Eventually, when you hold a dish up the the light and look from the back(agar) side, you will see individual sectors like the spokes on a wheel.

In the picture below, the dish on the left is the result of two transfers from the original spore swipe. Prior to the second transfer, the rhizomorphic mycelium was buried in the mass of strains and couldn't be differentiated, just like yours are.  The rhizomorphic sections are now clearly showing up at ten o'clock and two o'clock.  Each of those sections has four or five individual sectors that will be isolated out next time I do agar work in a day or two. The remainder of the dish will be discarded.  The dish on the right is a single sector isolate, as you can see the entire dish is rhizomorphic and when held up to the light, there is no further sectoring.  I fruit out each individual sector to determine the best fruiting isolate.  Many isolates are mediocre fruiters, but if you want to find the one that will deliver those monster flushes time and time again, this is how it's done.  I keep a small piece from each petri dish in the refrigerator until I've found the best fruiting isolate, then I get that piece out of the fridge and grow it out.  I always keep a piece from the original petri dish in test tubes in the refrigerator so I can go back to that strain for years to come with no fear of senescense.  It's a lot of work, but hey. . .It's a hobby right?
RR



https://www.shroomery.org/forums/showflat.php/Number/4377475#4377475

also there's this thread STRO's cleaning and isolating on agar TEK

so you see a clone has proven it's potential by growing, with isolates your going blind you need to test every isolate, some will suck
here's a good example
https://www.shroomery.org/forums/showflat.php/Number/18219379

Also a lot of noobs worry about rhizomorphic growth. Worry about healthy growth. you do not know if rhizomorphic growth will be any good till you test it. so tomentose growth may be a better fruiter or more potent, you won't know so don't concentrate on only rhizomorphic mycelium on dishes.

people get strains and varieties confused
A variety is a name on a syringe
A strain is two spores that have mated together

B+, GT, Cambo, Creeper, Penis Envy, KSSS, AA+, or any variety can grow weak, strong, tall, short, retarded, fast, etc...
B+(variety) is basically a mom and a dad(the name itself is the parent) and every time you squirt B+ into a jar they the name on the syringe has sex and has kids. your jars have 100s of kids your b+ parents made. Some of those kids are awesome some are stupid. Each of those kids is a strain.




Hope you all have a good day

Edited by make air go wooo (10/30/22 07:37 AM)

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Offlinemake air go wooo
Stranger

Registered: 12/02/21
Posts: 60
Last seen: 1 year, 9 months
Re: agar to grain using pins? [Re: make air go wooo]
    #28024146 - 10/30/22 08:30 AM (2 years, 2 months ago)

This might be a dumb question.  Lol

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 7,373
Loc: In the Gills
Last seen: 7 hours, 38 minutes
Trusted Cultivator
Re: agar to grain using pins? [Re: make air go wooo]
    #28024163 - 10/30/22 08:40 AM (2 years, 2 months ago)

Cloning is a very good thing to do. It narrows down the genetic pool away from MS. A plate pin makes a good clone. Some ppl like to clone fruits from a tub though so you can try to capture traits like size, color, prolific fruiting. My favorite culture I'm using came from a plate pin.


--------------------

Links For Beginners And Beyond:mspoil:

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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
Re: agar to grain using pins? [Re: rumfor69] * 1
    #28024175 - 10/30/22 08:47 AM (2 years, 2 months ago)

I've always gotten better pinsets and flushes when I run plate pin cultures vs MS. If this is your first grow I don't know that I'd suggest waiting an extra 2 months for a plate pin to show up though. Sometimes plates just will not pin no matter how long you give them.

If you do get a plate pin though you can just pluck the entire pin off and transfer it to a new plate, there's no reason to dissect it.



--------------------



I :heart: 5318008


NOT a virgin!

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