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Offlinematsc
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28025191 - 10/30/22 07:58 PM (2 years, 2 months ago)

Personally I go with pre-pours because I only have a 6qt pressure cooker, and it would drive me bonkers trying to fit a bottle of media in there...

But I can fit 10 'plates' at a time in it just fine, which is more than I really need at any given moment anyway.

The condensation is a little annoying, I will admit, but nothing too awful.


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InvisibleBaba Yaga
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Re: 70% iso doesn’t kill all bacteria [Re: matsc]
    #28025210 - 10/30/22 08:16 PM (2 years, 2 months ago)

Never had any problems with pouring in a SAB but I prefer no pours cause it's something that is more suitable to do alongside other stuff.

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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28025225 - 10/30/22 08:31 PM (2 years, 2 months ago)

Quote:

Envypenis said:
Okay that makes sense with the foil over the bottle
Neck. Before coming to shroomery and asking what I was doing wrong.. next option was Pyrex Petri dishes to attempt no pours. What other reasons do you justify the no pour?



I basically don't have a permanent place for my SAB, it gets put away after each session. I don't want to deal with timing the set up, worrying about temp, then having to let them cool


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho] * 2
    #28026466 - 10/31/22 04:23 PM (2 years, 2 months ago)

Alright so after digging into a few decent reads on this site.. I decided to ditch just about everything I “learned.” After reading just a few basic beginners threads here I noticed I made the ultimate mistake of YouTube and Reddit. I’m sure you guys must love Willy myco..

I need clarification on whether agar can caramelize if over cooked or re sterilized? Quick google search tells me sugars don’t start to Carmelize until 336* .. I’ve found some contradicting answers on this one.

I ditched the quart jars I had made up the night before, and re bottled them. I had quart media jars in my cabinet the whole time. Loosened cover before PC and covered with foil. 40 mins pressure and just a CH above zero I released pressure and as quickly as possible Sealed the media bottles. Will get 500 ml soon didn’t like having to tip them over in the PC but it worked.



I made up my 100 qt rigid still air box with nice roomy 8” arm holes. Very comfortable now I guess the 66 qt was my downfall Before. I have the agar in the water bath now at 120*. Have a few more questions before I start tonight..

Towel or no towel? The edges of the tub don’t sit flat on the table. 1/8” gap in areas. Towel would make an even seal but again I don’t know if that’s better or worse.

I’m out of Petri dishes and have to resort to using the Jell-O shot cups. Going to make it interesting. Thinking I’ll lay out the cups with lids resting on top one by one then go ahead and pour. Can easily see the advantages here now of the dishes.

I can also see why the tent would be worse for SAB work. I swear if I breathe I can see the walls move a minute amount never mind moving my body in this bitch. I’ll be very much paying attention this round. Appreciate all the constructive criticism and eye openers.. seriously woke me the fuck up

Edited by Envypenis (10/31/22 04:25 PM)

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Offlinehazyhorse
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28026481 - 10/31/22 04:32 PM (2 years, 2 months ago)

glad we could help man! thanks for taking the advice to heart. i think it was a good idea to get rid of all your prior “knowledge”

i’ve never had problems resterilizing agar a few times, you should be fine

i’ve seen people on here recommend the towel on the bottom. i wouldn’t work directly on the towel obviously, but if it’s gonna seal your SAB better on the bottom i think it’s a good idea. i’ve heard it helps contams from stirring up (as long as you’re not moving the towel around or something obviously)


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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: hazyhorse] * 1
    #28026491 - 10/31/22 04:37 PM (2 years, 2 months ago)

the damp towel is a classic trick, i assume a dry towel would work. get yourself a nice big rack that fits the sab. like an oven rack or cooling rack for bakes goods, something like that

dont worry about agar carmelizing


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: hazyhorse] * 1
    #28026498 - 10/31/22 04:39 PM (2 years, 2 months ago)

Thank you this place really is amazing. What I read last night I’m sold. You all are changing the world with this at home science. It’s incredible. Loved the part about we basically are all committing felonies don’t get butthurt over simple words! I understand it all. Especially squashing bad info.. to make sure good info persists. I’m sure I’ll be a life long member now

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Offlinehazyhorse
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28026504 - 10/31/22 04:41 PM (2 years, 2 months ago)

you’re gonna fit in great around here. excellent attitude to have my friend. welcome to the shroomery :cheers:


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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InvisibleBaba Yaga
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28026506 - 10/31/22 04:41 PM (2 years, 2 months ago)

Quote:

Envypenis said:


I made up my 100 qt rigid still air box with nice roomy 8” arm holes.





That is a nice SAB with well sized arm holes :thumbup:


What does the lid of this tub look like? Some can be used as SAB floors if they are flat and without any weird grooves and such nonsens.

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings]
    #28026513 - 10/31/22 04:45 PM (2 years, 2 months ago)

Damn always one more thing.. plastic bags hung over the arm holes. Do you guys tape them on and remove when you go to work?  roll them up out of the way? Tape from the side and fold off to the side when working? I don’t think I’ll have to worry about this tonight as the Jell-O shots have a snap top. But when I do get Petris again you hang the bags while waiting for the dishes to cool before parafilm correct?

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: Baba Yaga]
    #28026515 - 10/31/22 04:46 PM (2 years, 2 months ago)

Unfortunately it’s a pain in the ass cover. Ridges and a huge bow in the middle somehow. I really couldn’t get the rack to sit flat without it being dead center taking up most of the space

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Offlinehazyhorse
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28026521 - 10/31/22 04:51 PM (2 years, 2 months ago)

tbh i just covered my armholes by draping a t shirt  off the top & slightly pushed the tshirt in the holes. there is probably a more elegant solution but it worked for me


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: hazyhorse]
    #28026531 - 10/31/22 04:59 PM (2 years, 2 months ago)

yea same i drape a big towel over it. i imagine plastic covers taped on would be a pain to deal with


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings]
    #28026732 - 10/31/22 06:52 PM (2 years, 2 months ago)

Perfect even more simple.. just finished pouring 1.6L of agar. I tried one bottle no towel, one bottle with towel. I can see how the mist would also help to catch the contam so why not try both on the stainless table. I’m definitely still in the experimental phase. Gave me a chance to wipe everything out again and start fresh in between batches. Very much looking forward to Petri dishes but I don’t think it went horribly with Jell-O shots. Only thing I didn’t really like was I Couldn’t press the containers closed on the rack. I had to move it down to the cutting board to press closed. But all was covered before moving down to contamination land. Honestly I was at 10-30% success rate before so I’d say anything will be better

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: hazyhorse]
    #28026742 - 10/31/22 07:03 PM (2 years, 2 months ago)

What’s your opinion on flipping agar dishes? Always flip? Only flip after pouring? Or flip when you have inoculated with agar wedge also? And if you flip with agar wedge how long would you let it set to adhere to the plate? need to dig a little deeper myself into this topic but have found contradicting answers again I believe. I was flipping agar cups with agar wedges and they kept falling onto the lid.. Never really could find an answer how long you let them adhere but seemed like overnight worked.. 

Edited by Envypenis (10/31/22 07:05 PM)

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Invisiblebigfootscreepyuncl
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28026748 - 10/31/22 07:06 PM (2 years, 2 months ago)

I've never once flipped a plate. I don't think that is common practice in this hobby


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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: bigfootscreepyuncl]
    #28026755 - 10/31/22 07:09 PM (2 years, 2 months ago)

Quote:

bigfootscreepyuncl said:
I've never once flipped a plate. I don't think that is common practice in this hobby



Maybe 20 years ago


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: bigfootscreepyuncl]
    #28026760 - 10/31/22 07:10 PM (2 years, 2 months ago)

Awesome thank you that was what I pretty much got when I did look into it. For bacterial cultures you would flip, not for mycology. Definitely helps ease the mind

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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28026776 - 10/31/22 07:18 PM (2 years, 2 months ago)

Quote:

Envypenis said:
Awesome thank you that was what I pretty much got when I did look into it. For bacterial cultures you would flip, not for mycology. Definitely helps ease the mind



I don't know about growing bacteria, I hear it's quite challenging to do it properly. For our purposes though there's really no need to flip. The water in your plates should be just as sterile as the surface of the agar.

I put plates with condensation at the bottom of the stack and if I have the opportunity before they dry I'll use them for spore germination


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho]
    #28026783 - 10/31/22 07:22 PM (2 years, 2 months ago)

That’s a helpful tip I’ll go rotate the condensated cups to the bottom right now. My first batch I was at 127* when I went down and had condensation. The second batch was 117* and not a drop of condensation on any. I’ll definitely be replicating that from here on out

Edited by Envypenis (10/31/22 07:23 PM)

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