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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: bigfootscreepyuncl]
    #28024974 - 10/30/22 06:24 PM (2 years, 2 months ago)

Quote:

bigfootscreepyuncl said:
personally, I'd suggest getting good at a SAB before that kind of investment. For one, if the FFU breaks or you're out of power and need to work you better know how. But also, and probably more important, make sure that you actually enjoy this hobby before dropping that kinda cash on it. That's just my $0.02 - do what you want, I couldn't actually care less :lol:



:whathesaid:
totally agree

and also now that im rocking a ffu, im finding that certain things were easier in a sab. stillness is incredibly useful


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho]
    #28024979 - 10/30/22 06:27 PM (2 years, 2 months ago)

Correct but I do feel I will be much more comfortable working where I can see and move without being hunched or kneeling.. I hate looking thru the plastic box also. As long as you keep your clean materials upstream of dirtier materials I don’t see much else to go wrong.. sterilization of tools and that’s about it?

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho]
    #28024985 - 10/30/22 06:29 PM (2 years, 2 months ago)

Could you expand upon the idea of sterile technique? What did I miss? This is where confusion was found as a beginner because sterile seems to be the goal But apparently is impossible

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings]
    #28024992 - 10/30/22 06:31 PM (2 years, 2 months ago)

What In your opinion is easier in the sab vs a flow hood?? That type of info would be great for others to find in the future

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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis] * 1
    #28024994 - 10/30/22 06:31 PM (2 years, 2 months ago)

Quote:

Envypenis said:
As long as you keep your clean materials upstream of dirtier materials I don’t see much else to go wrong.. sterilization of tools and that’s about it?



Yep, it's that simple. Just like SAB work is as simple as not stirring up the nasties.



Quote:

Envypenis said:
move without being hunched or kneeling



Sounds like user error to me. Put your box in a more comfortable spot.


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho]
    #28025008 - 10/30/22 06:34 PM (2 years, 2 months ago)

Easy enough to fix. I’m going to take my time building this one now that I have a workplace I call home.. Was a shit show in the beginning.. still a shit show but atleast had a decent harvest last month. Revived my hopes and it’s not just pushing thru hell anymore. Love your quote on balance .. very acccurate!

Edited by Envypenis (10/30/22 06:37 PM)

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Offlinethe man
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis] * 2
    #28025026 - 10/30/22 06:39 PM (2 years, 2 months ago)

awesome love to see folks not get POd when folks tell them they are doing it wrong!

nope just read about SAB and teks. move slow take your time, dont put hand over anything, I like to rub everthing down JUST incase, give time for things to settle. assume your hand rains down dirt and that everthing is covered in thick uber fine dust that will get stired up with any air movement.

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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis] * 1
    #28025030 - 10/30/22 06:40 PM (2 years, 2 months ago)

Quote:

Envypenis said:
What In your opinion is easier in the sab vs a flow hood?? That type of info would be great for others to find in the future



for me doing small things like agar transfers is more natural with still air but for big movements like pouring LC not being in a box is nice


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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: the man]
    #28025033 - 10/30/22 06:41 PM (2 years, 2 months ago)

Quote:

the man said:
awesome love to see folks not get POd when folks tell them they are doing it wrong!




:whathesaid:

there are two types of people :/


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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis] * 1
    #28025036 - 10/30/22 06:42 PM (2 years, 2 months ago)

Quote:

Envypenis said:
Could you expand upon the idea of sterile technique? What did I miss? This is where confusion was found as a beginner because sterile seems to be the goal But apparently is impossible



Sure, I absolutely can.

The goal of sterile technique/aseptic work practices is to ensure we don't cause anything floating around to float into our sterile containers.

It's important to understand what is sterile and what is not. The insides of containers that have been pressure cooked are sterile, the outside isn't. The parts of our tools exposed to flame are sterile, the rest isn't. Plates sealed in sleeves from the manufacturer are also sterile.

We don't accomplish our goal of keeping the nasties floating around from getting into our containers by cleaning them away because no matter how hard we try we won't get everything and all it takes is a few spores.

We accomplish our goal through careful handling. In an SAB this pretty much means you never really want to open containers fully, you only lift lids enough to get your tools in there. The times that you do need to completely remove lids, nothing passes over the open containers.

I haven't read your posts in detail but from skimming it seems your problem is the air in the tent is moving and causing the air in your box to move. The room you work in should also be allowed to settle. Any windows, vents, fans should be shut, plugged, turned off.


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: the man] * 1
    #28025054 - 10/30/22 06:47 PM (2 years, 2 months ago)

Visualization is a great tool! That will help for sure when I’m down there. Thanks to everyone who chimed in. I mean when you’ve tried everything.. and failed over and over again.. it’s a pretty humbling experience. People who claim ego death on mushrooms.. should try growing it first 🤦♂️

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho]
    #28025070 - 10/30/22 06:50 PM (2 years, 2 months ago)

Perfect thank you! For whatever reason I had way too much trust in alcohol to kill the bad stuff. I guess the hand sanitizer push the last few years made it seem more effective. I’m sure some of you guys have scientific backgrounds but I’m assuming there is a pretty big learning curve with this field of study…

I see people using containers like whiskey bottles for pouring which will on the list, smaller mouthed pc safe bottles... But how crazy do I have to be about this. If I lift the lid of the agar, to pour, how could I not get the jar over the dish if pouring into dish below?

Edited by Envypenis (10/30/22 06:53 PM)

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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28025087 - 10/30/22 06:56 PM (2 years, 2 months ago)

Quote:

Envypenis said:
Perfect thank you! For whatever reason I had way too much trust in alcohol to kill the bad stuff. I guess the hand sanitizer push the last few years made it seem more effective. I’m sure some of you guys have scientific backgrounds but I’m assuming there is a pretty big learning curve with this field of study..



The key word there is sanitizer. It's not a sterilizer. Sanitary means 99.9% of germs are killed. We need 100%. Only fire and steam/pressure do that, UV too but not for any application that is practical to us really.

The other thing is germs(I.E. shit that has GERMINATED) aren't what we're worried about cause those are easy to kill, it's the spores that haven't germinated that we're worried about. Very few things are sporucidal. Alcohol certainly isn't.


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho]
    #28025090 - 10/30/22 06:57 PM (2 years, 2 months ago)

idk bro thats why i stick to no-pours

regarding sterile technique
just imagine that any unsterile surface is covered in contam that can transfer by contact, any air that isnt laminar flow or completely still is full of contam that wants to land in your precious container, and any unsterile object you wave around can shed contam either down (SAB) or towards you (LFH)

you are playing goalie for a sterile food source, against invisible balls


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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28025091 - 10/30/22 06:58 PM (2 years, 2 months ago)

Quote:

Envypenis said:
I see people using containers like whiskey bottles for pouring which will on the list, smaller mouthed pc safe bottles... But how crazy do I have to be about this. If I lift the lid of the agar, to pour, how could I not get the jar over the dish if pouring into dish below?



Generally people keep the necks wrapped in foil from the pressure cooker until pouring, keeping the part that will be over plates sterile


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

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"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings]
    #28025094 - 10/30/22 06:59 PM (2 years, 2 months ago)

Quote:

bakedbeings said:
i stick to no-pours




:whathesaid:

For different reasons though.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SirPsycho]
    #28025136 - 10/30/22 07:23 PM (2 years, 2 months ago)

Okay that makes sense with the foil over the bottle
Neck. Before coming to shroomery and asking what I was doing wrong.. next option was Pyrex Petri dishes to attempt no pours. What other reasons do you justify the no pour?

Edited by Envypenis (10/30/22 07:23 PM)

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Offlinehazyhorse
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis] * 1
    #28025157 - 10/30/22 07:34 PM (2 years, 2 months ago)

no pour is nice bc i don’t have to worry about my sterile technique while pouring since they come out of the PC sterile. you have to PC the agar anyways so why not just get it done all at once? also you’re not constantly tossing petri dishes this way. glass petris offer pretty sick visibility but even traditional pasty plates kick ass.


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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: hazyhorse] * 1
    #28025174 - 10/30/22 07:44 PM (2 years, 2 months ago)

Quote:

hazyhorse said:
no pour is nice bc i don’t have to worry about my sterile technique while pouring since they come out of the PC sterile. you have to PC the agar anyways so why not just get it done all at once? also you’re not constantly tossing petri dishes this way. glass petris offer pretty sick visibility but even traditional pasty plates kick ass.



:rockon:


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings]
    #28025188 - 10/30/22 07:55 PM (2 years, 2 months ago)

Definitely going in the no pour direction when i can. I do love being able to see what I’m working with. It just makes sense anyways. One less possible chance of contamination. I’ll get back to you all by tomorrow night with results. Praying I’ll see an improvement. Going to be extremely anal about this batch

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