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Offlinechris77
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Re: 70% iso doesn’t kill all bacteria [Re: Grind365]
    #28023950 - 10/30/22 02:29 AM (2 years, 2 months ago)

:laugh2:


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: chris77]
    #28024021 - 10/30/22 05:42 AM (2 years, 2 months ago)

First off I have to thank you all for quick responses. I tried on Reddit last year, let’s just say this forum blows it away.

I was trying to write that quick before I had to go to bed last night. Work seven days a week no rest for the wicked. I’ll put up some pictures first to clarify then go thru the replies..



“Still air box”.. decent arm holes I thought. Makes a good seal around my arms with lab coat on hence why I got it. I’ll have to pay attention next time and maybe video so you all can see but I really don’t jotice much movement of the material while I’m working. Obviously make a huge reach and there’s a chance. I will pay more attention to speed inside the air box. Read about a few guys going in to do agar work naked. That might be enough to keep me aware of what I’m supposed to be doing in terms of not making quick movements.



This is the air inlet. 6” pvc pipe with correction merv 16 rated filter paper tightened down with hose clamp. Outside is on a carbon filter mounted fan made for weed. The idea was to push positive pressure in the tent so when I would leave a small vent open to let out alcohol fumes it would not drag back in particles. I call the tent my lab, and thought I had a SAB.

The consensus seems to be too much movement within the SAB. I have a 100qt box downstairs I’ll heat cut a couple arm holes tonight, kill all air movement in the tent, and see if it makes any difference. Really can’t thank you all enough on the responses. Flow good is next. Is it generally considered acceptable to work quicker in front of a flow hood? I’m doing 100+ dishes a week and already lacking on time





Hillbilly done in that “SAB”.. I had perfect results in this thing originally. I mean to the point I was confident enough I was pouring 10-15 jello shots at once before putting on covers with near perfect success. I’d Try not to cross over the cups but still happened with better success rates than now. The only other thing that changed was going from pint jars to quart jars but already dealt with that - headspace issue so I thought. Seems to be better now as success rates did improve 30% or so.

Guess I’ll be improving on still air technique vs sterile






Edited by Envypenis (10/30/22 06:07 AM)

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OfflineKROM
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis] * 1
    #28024036 - 10/30/22 06:16 AM (2 years, 2 months ago)

SAB tek by bod. Making armholes with a coffee can = chef’s kiss.


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OfflineSexBurrito
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28024054 - 10/30/22 06:53 AM (2 years, 2 months ago)

Definitely try a rigid plastic box and kill the fan.  I don't even wear gloves in mine nor do I set it up in my tent anymore and it's works out great, I'd say 95+% of the time.  People would cringe at how I do things but I rarely get contamination. Oddly enough I've also done it naked and didn't really see any difference overall though. Mushrooms and agar work are super easy once you dial in what works for your environment. Your on the right track to succeeding, best of luck


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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: SexBurrito]
    #28024060 - 10/30/22 07:00 AM (2 years, 2 months ago)

just here to add that you dont want tight cuffs for arm holes. you want wide comfy armholes much bigger than your arms so you can move in and out of them without disturbing the air. i know its counterintuitive but its very well tested


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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: Baba Yaga]
    #28024063 - 10/30/22 07:04 AM (2 years, 2 months ago)

Quote:

Baba Yaga said:
You have this POS right?



"designed with you in mind"



"hey! do you know nothing about SABs? then we designed this just for you!"


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Confused? Well now you can!
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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Baba Yaga]
    #28024073 - 10/30/22 07:13 AM (2 years, 2 months ago)

Quote:

Baba Yaga said:
You have this POS right?




This is the reason for your failure. You will be better off throwing this disgrace of SAB away. I can't believe those ass wipes are actually selling something like this and their advertising is making me throw up, "designed with you in mind", yeah right.

Sorry for the rant it's not your fault, but honestly get rid of this thing and build a SAB out of a rigid plastic tote. Reduce your alcohol use to a minimum and rely on good sterile technique......you know, the kind of technique you weren't able to achieve with this floppy SAB these dickheads sold to you.



Oh fuck me sideways, I'd rather do open air transfers than use that trash bag.... Hell I'll take an actual trash bag over that


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Invisiblebigfootscreepyuncl
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28024165 - 10/30/22 08:40 AM (2 years, 2 months ago)

I never understood why people are putting a SAB inside a tent..I guess it might be useful if you're unable to turn off a fan/air registers in the room, otherwise, think of the SAB as a tiny little tent for your work.

Ditch the flexible SAB or at the very least cut bigger arm holes in it. As others have said you do not want the 'cuffs' to form a seal around your arms. The ONLY thing those SABs have going for them is the clarity. Other than that it's trash.

Quote:

Envypenis said:Read about a few guys going in to do agar work naked.




I'm a nudist and even I can't see a benefit of doing lab work naked


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OfflineDave Bowman
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Re: 70% iso doesn’t kill all bacteria [Re: bigfootscreepyuncl]
    #28024259 - 10/30/22 09:59 AM (2 years, 2 months ago)

Just build a legit SAB made out of a hard plastic.  They are super cheap.

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: bigfootscreepyuncl]
    #28024291 - 10/30/22 10:31 AM (2 years, 2 months ago)

The naked part I don’t think would apply to you as a nudist lol As a non nudist I’d be thinking dude you’re naked with every wisp of air that goes by keeping me constantly thinking slow down. The plastic air box I had a year ago then went to open air transfer in the tent, and I guess didn’t put it together that sealed was worse so went with that vinyl set up I think for the size and clarity. I work in my basement so the tent helps me keep a clean area without getting into concrete walls and rafters with dusts/molds everywhere. I also keep my agar in there and temps set to 80*.  Now if the issue is movement of the air box walls.. what if you cut arm holes big enough not to touch the flexible sides at all? And really maintain not touching the sides.. slow down and have deliberate planned and perfectly executed movements within the air box. Is there a write up for still air box work or procedure? Haven’t had time to look while im at work today but need answers when I get home. Quickly replying as I can to keep answers coming

Edited by Envypenis (10/30/22 10:32 AM)

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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis] * 1
    #28024308 - 10/30/22 10:52 AM (2 years, 2 months ago)

logically flexible walls arent ideal for an apparatus with the sole  purpose of staying still, but seeing as you already bought the thing you are welcome to cut wider holes and see if that helps

most people here will correctly point out that a real SAB is so easy and cheap that theres no reason not to get it over with and just fuckin make one. you can keep that other thing around for incubation, use it as a cat condo, or sell it to the next sucker

i like the idea of a tent as a workspace if drafts in the room are hard to control. otherwise its just a placebo-tent

and to whoever suggested using a flow hood in a closed tent would create turbulence...remember that the hood wont even create turbulence at point blank range. that is a key feature of its design


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Offlinehazyhorse
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings] * 1
    #28024442 - 10/30/22 12:54 PM (2 years, 2 months ago)

if you have $500 and are in the US/somewhere amazon has the deal, there are FFUs that work as flowhoods. 100000x better than an SAB to work in, would make your work very quick. but if not, the hard plastic SAB is the way to go


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OfflineSexBurrito
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Re: 70% iso doesn’t kill all bacteria [Re: hazyhorse] * 2
    #28024700 - 10/30/22 03:50 PM (2 years, 2 months ago)


Ol' faithful.  Cost like 14$ and works fantastic.  Don't have the money for a flow hood just yet but this just proves that if your method is tight, rudimentary equipment like this will work.  Good luck brother


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: SexBurrito]
    #28024806 - 10/30/22 05:08 PM (2 years, 2 months ago)

Really appreciate the help you guys. I knew I was missing something simple but I hate asking for help until I’ve tried everything I can think of. I grew up working in a shop no one had time to answer questions this is just how I handle things now. Still driving home from work right now. I’m going to do a thorough search into still airbox techniques as soon as I’m home but does anyone have anything to add I may not see in the teks?

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: hazyhorse]
    #28024852 - 10/30/22 05:34 PM (2 years, 2 months ago)

Inoculate the world had a 2x4 FFU for $1050. That’s my next planned investment. Anyone see anything wrong with that before I make another terrible buy ? Otherwise I’ll go all out and get a lab type set up. Expensive sure.. Had found lab type horizontal flow hoods for around 3300 just a few months back. Can’t seem to find it now. but with all the time I put into this, with lack of time.. seems to be the only option. Plus I’m too stupid to ever quit. Flow hood definitely won’t go to waste

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Invisiblebakedbeings
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28024920 - 10/30/22 06:06 PM (2 years, 2 months ago)

ah yes...youre learning. ask before you do things. it took me too long to figure that out

regarding flow hoods i have the YJINGRUI FFU from amazon. i like it a lot, but if i could afford a 2x4 model i would go for it


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Invisiblebigfootscreepyuncl
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings] * 1
    #28024949 - 10/30/22 06:15 PM (2 years, 2 months ago)

personally, I'd suggest getting good at a SAB before that kind of investment. For one, if the FFU breaks or you're out of power and need to work you better know how. But also, and probably more important, make sure that you actually enjoy this hobby before dropping that kinda cash on it. That's just my $0.02 - do what you want, I couldn't actually care less :lol:


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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: bakedbeings] * 1
    #28024950 - 10/30/22 06:16 PM (2 years, 2 months ago)

Always learning .. usually the hard way…

I just read the SAB tech by bod It didn’t take much to click. Really wish I could have seen a year ago. Always have to pay some tuition in the school of hard knocks

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OfflineEnvypenis
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28024958 - 10/30/22 06:18 PM (2 years, 2 months ago)

No worries on that I have a tendency to become obsessed with things and my life will shortly revolve entirely around it.. Practically already does just like to see more come back lol i’m showering now because I leave for work Irish and come home Haitian. I already have another qt of agar warming up in the water bath and will be going down to get my hundred quart tub going to take one of my pots in the kitchen to heat it up about 8 inches and press it through for arm holes and see what the hell happens. I’ll follow the procedure from the tech really take it down to super slow motion. Only change might be i think I’m going to use Lysol to sanitize because they claim 99.9% of germs killled vs alcohol 67% .. Is that going to ruin everything?

Edited by Envypenis (10/30/22 06:22 PM)

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OfflineSirPsycho
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Re: 70% iso doesn’t kill all bacteria [Re: Envypenis]
    #28024964 - 10/30/22 06:20 PM (2 years, 2 months ago)

A flow hood isn't a replacement for sterile technique. Working in front of a flow hood needs just as careful handling as an SAB, the specifics of sterile technique in front of a hood vary quite a bit compared to in an SAB because the two work on almost completely different concepts.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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