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OfflineCaeserMalone
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Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold?
    #28021928 - 10/28/22 06:44 PM (2 years, 2 months ago)

So I inoculated this 5 days ago. It definitely looks like mycelium forming are the black clumps but what worries me is that the black clumps seem to be getting bigger and bigger each day which makes me think it could be black mold in my jars. What do you guys think?

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OfflineDitchdude
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone]
    #28021935 - 10/28/22 06:50 PM (2 years, 2 months ago)

I don't see a photo

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OfflineCaeserMalone
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: Ditchdude]
    #28021942 - 10/28/22 06:55 PM (2 years, 2 months ago)


Edited by CaeserMalone (10/28/22 06:58 PM)

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OfflineCaeserMalone
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone]
    #28021943 - 10/28/22 06:56 PM (2 years, 2 months ago)


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OfflineCaeserMalone
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: Ditchdude]
    #28021945 - 10/28/22 06:57 PM (2 years, 2 months ago)

I reuploaded them. Not sure why they didn't post the first time.

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Invisiblebakedbeings
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone] * 1
    #28021955 - 10/28/22 07:06 PM (2 years, 2 months ago)

how did you inoculate?
:anticipation:


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OfflineLand TroutM
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: bakedbeings]
    #28021990 - 10/28/22 07:37 PM (2 years, 2 months ago)

Molds typically float don’t they?  Doesn’t look right, looks like spores, I’m no expert with liquid culture.

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OfflineCaeserMalone
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: bakedbeings]
    #28022036 - 10/28/22 08:15 PM (2 years, 2 months ago)

With a gt mss. It was about 2ml

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OfflineCaeserMalone
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: Land Trout]
    #28022037 - 10/28/22 08:16 PM (2 years, 2 months ago)

I have no idea. This is my first time with LC. I guess I'll have to get some agar to test it out.

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OfflineKROM
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone]
    #28022068 - 10/28/22 08:42 PM (2 years, 2 months ago)



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OfflineLand TroutM
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone] * 1
    #28022072 - 10/28/22 08:47 PM (2 years, 2 months ago)

Yeah, don’t shoot spores into nutrient broth and expect it to be clean.

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OfflineCaeserMalone
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: Land Trout]
    #28022084 - 10/28/22 09:01 PM (2 years, 2 months ago)

How else do you do a liquid culture? I thought it was typically done with a mss.

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OfflineLand TroutM
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone]
    #28022135 - 10/28/22 09:30 PM (2 years, 2 months ago)

Get a clean culture on agar first.  You could get a clean LC from spores, but it’s a roll of the dice. 

Edited by Land Trout (10/28/22 09:30 PM)

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Offlineechochild
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone]
    #28022145 - 10/28/22 09:38 PM (2 years, 2 months ago)

Quote:

CaeserMalone said:
How else do you do a liquid culture? I thought it was typically done with a mss.




MSS is inherently "dirty". It was made from spores that were naturally taken from a potentially bacterial and/or moldy environment.

Injecting something with a high likelihood of contamination into a nutrient rich broth, the contaminates are very likely to out compete the spores, and you will end up with a jar of contamination instead of mycelium.

Did you watch the video of Willy where he said injecting MSS to LC was ok?

For future reference, listen to the others in the post. If you want a higher chance of a clean LC, start the MSS on agar first so you can spot, and isolate away contaminants. When you finally have an agar transfer that is clean, then you can add that to an LC.

I have a GT LC running right now that was from a spore print -> agar -> Transferred 3x to clean it up, then added to an LC jar. It's awesome, and colonized quick, but it needs to be done right.

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Invisiblezmicroinquisitor
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: CaeserMalone]
    #28022150 - 10/28/22 09:40 PM (2 years, 2 months ago)

Looks eerily similar to a contam mss I had


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Invisiblebakedbeings
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Re: Inoculated karo liquid culture 5 days ago. Does this look normal or like black mold? [Re: Land Trout]
    #28022546 - 10/29/22 09:08 AM (2 years, 2 months ago)

Quote:

Land Trout said:
Yeah, don’t shoot spores into nutrient broth and expect it to be clean.



:whathesaid:

if you want to use spore syringes, do PF TEK

if you want to go bulk, grains, LCs, etc, agar is the way


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