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OfflineGhstblz3
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Registered: 09/30/22
Posts: 28
Last seen: 1 year, 11 months
First time inoculations with agar
    #28022294 - 10/29/22 12:47 AM (2 years, 2 months ago)

Hey guys, I’ve been cultivating for a couple of years now and I was recently told I’ve been doing it wrong the whole time .
I’ve always just used sterile grain bags with self healing ports , and over those few years I’ve only have had 2 total bags with contamination issues.

I recently started using a different tek than what I was taught by my mentor , so I came here for tips and this is where I was told I should be innoculating with pre-poured agar trays.
I shot some spores onto the plates, and popped them in my incubator.

So my main question/concern is After I get mycelium one the agar plates, how exactly do I get it into the bag? Just cut the bag open and drop it in? Ate there any helpful
Quality of life techniques to help with this process?
Also, if it is done by cutting the bag open, how do I seal the it back up after dropping the mycelium in?

Thanks in advance, any tips appreciated .


Stay wavy friends 🙃

Edited by Ghstblz3 (10/29/22 12:51 AM)

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Offlineechochild
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Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
Re: First time inoculating grain with agar [Re: Ghstblz3]
    #28022300 - 10/29/22 12:54 AM (2 years, 2 months ago)

Edit: After I get a clean transfer (usually it take me like 2-3 transfers... but I found even then it's not always necessary), you can cut up the agar and just drop it in the opened bag. Some people here just drop the hole plate, some cut the plate into wedges. I tend to cut it up into 8 wedges so they spread out in the bag, and I've noticed faster colonization times so far.

Edit 2: Make sure you are being sterile when you open the bag, the agar, and in dropping the agar in. I don't know if your mentor has shown you an SAB or if you already know about them?

For resealing the bag: If you feel like dropping the money, you can get an impulse sealer. I got one on Amazon for ~$40 USD.

If you have a Pressure Cooker, you could also shift away from bags, and start using jars and make your own grain spawn. You don't waste plastic, they are reusable, and the work on them is pretty simple. Don't know if you want to go down that far into the rabbit hole though.

Edited by echochild (10/29/22 01:03 AM)

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OfflineGhstblz3
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Registered: 09/30/22
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Last seen: 1 year, 11 months
Re: First time inoculating grain with agar [Re: echochild]
    #28022307 - 10/29/22 01:13 AM (2 years, 2 months ago)

Well I was doing pf tek cakes , I just harvested my second flush from my first monotub. if those are the cakes you’re talking about.

I have a Martha tent setup including all the electronics coming in, should be here any day now.
What does SAB stand for?

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Offlineechochild
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Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
Re: First time inoculating grain with agar [Re: Ghstblz3]
    #28022314 - 10/29/22 01:24 AM (2 years, 2 months ago)

Quote:

Ghstblz3 said:
Well I was doing pf tek cakes , I just harvested my second flush from my first monotub. if those are the cakes you’re talking about.

Sorry... slightly confused now. You were using PF Tek and someone told you that was wrong, or were you shooting multispore syringes into the grain bags?

I have a Martha tent setup including all the electronics coming in, should be here any day now.

Oh boy... you are going to find a lot of haters on automated systems (for good reasons though). I have a grow tent myself, but it's primary function is to keep one of my asshole cats OUT of messing with my shrooms. Most of the electronics aren't needed, as long as you are willing to be even minimally attentive to your grows (like 1-3x per day depending on your climate)

What does SAB stand for?

Still Air Box. It's used to try to help cut down on airflow when working on stuff that needs to stay as sterile as possible. Opening spawn bags in open air, agar too, can lead to contamination. Doing that work in a SAB can lower the risks. There's tons of info on SAB's, and construction here (there s even one thread going on where we were all discussing the merits of cutting holes vs. melting them. They are cheap to make.





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