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ChocyMilky
Budding Mycologist



Registered: 03/30/20
Posts: 31
Last seen: 1 year, 11 months
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Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience?
#27962764 - 09/22/22 04:06 PM (2 years, 3 months ago) |
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Check out the pictures. I inoculated a number of jars with two different LC's that were prepared from a clean agar wedge of two different strains behind a flow hood. The agar produced clean jars but the LC does not appear to me to be clean once put to grain.
Is this bacterial contam? Do you have experience with exactly this?
(more details) The agar and prepared grain are Clean. I can say that definitively because i did a side by side knocking up grain with the agar wedges used for the LC.
From these picture of the grain spawn do you agree the LC is dirty and is this bacteria? At what point is this contam coming into the LC?
I pressure cooked these LC's for 45 minutes, then they sat for 10 days, then an agar wedge went in and then they were colonized over the next 15 days or so before going to grain..and looked crystal clean
I must be contaminating these LC's when I am adding the wedge..no matter what I believe regarding how good my sterile technique is..I don't know what other explanation there is.
Would you spawn this? My experience is telling me no.



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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,563
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: ChocyMilky]
#27962779 - 09/22/22 04:14 PM (2 years, 3 months ago) |
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do u have pics of the other jars to compare?
first thoughts - introducing liquid is another variable. there could be bacteria in all the jars but its only taking off in the LC ones because of all the extra moisture
ive spawned worse jars than that...show them at 100% and we'll talk
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SirPsycho
Purple Belt in Google-Fu



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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: bakedbeings]
#27962781 - 09/22/22 04:16 PM (2 years, 3 months ago) |
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No, I'd let that colonize then case and fruit as is if I really had to.
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Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens
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Forrester
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: ChocyMilky]
#27962800 - 09/22/22 04:32 PM (2 years, 3 months ago) |
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Yeah wait till 100% and see how slimy it is then. As it looks now I'd spawn it, I've certainly spawned worse.
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bigfootscreepyuncl
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: Forrester]
#27962877 - 09/22/22 05:27 PM (2 years, 3 months ago) |
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You might have better results if you work in front of your flowhood...just sayin' 
Have you tested your LCs on agar?
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: bigfootscreepyuncl]
#27962884 - 09/22/22 05:30 PM (2 years, 3 months ago) |
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Quote:
bigfootscreepyuncl said: You might have better results if you work in front of your flowhood...just sayin' 
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Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens
Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
   
"Mist your balls and fan your asshole" - Pandaskis, 2023
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fiddle_head
fettucine embargo



Registered: 08/05/08
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: ChocyMilky]
#27963118 - 09/22/22 07:22 PM (2 years, 3 months ago) |
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Those grains look bacterial for a number of reasons. You can only wait it out but my guess chances are you’ll know if it’s trash before it’s fully colonized.
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ChocyMilky
Budding Mycologist



Registered: 03/30/20
Posts: 31
Last seen: 1 year, 11 months
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: bakedbeings]
#27963126 - 09/22/22 07:24 PM (2 years, 3 months ago) |
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Quote:
Bakedbeings said: do u have pics of the other jars to compare?
first thoughts - introducing liquid is another variable. there could be bacteria in all the jars but its only taking off in the LC ones because of all the extra moisture
ive spawned worse jars than that...show them at 100% and we'll talk
The 4 pictures are of 4 separate jars. There's a few dozen jars, they all look like these. What I am trying to capture here is rather subtle for a photo, it is much more obvious when viewed in person. The second picture is probably the best reference, you can really see the discolored couple of grain and surrounding somewhat grayer grain.., and then the much whiter mycelium establishing itself growing upward. In the 3rd photo you can probably glean the discolored area is where the LC started and you see the newest mycelium growing away from it is white and healthy looking..hopefully this demonstrates the disparity.
Certainly going to liquid is a big part of the process and a large vector for contamination. I believe what we are seeing is ?bacteria? that has been introduced to the LC, taken hold there, and then proliferating in the grain.. I believe the grain itself is not the vector for contamination..its just subtle and not what I can clearly identify as an obvious yellow bacteria colony. That's why I am opening this up for discussion in case one of you peeps is like BAM yeah been there here's exactly what that is and whats going on there.
I've spawned every type of jar..my experience is telling me if i shake these jars up I'm basically spreading the contam in proportion to the "healthy" mycelium, both will continue to proliferate when I go to bulk ultimately I'll end up with weak mycelial block that will be prone to early contamination if it gets to any fruiting at all..just not worth the time and materials.
I'd really really like to know what this contam is, what type of bacteria if its bacteria or is is yeast or what..I don't know this one?
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ChocyMilky
Budding Mycologist



Registered: 03/30/20
Posts: 31
Last seen: 1 year, 11 months
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: SirPsycho]
#27963146 - 09/22/22 07:30 PM (2 years, 3 months ago) |
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Thank you for your input. Solid advice. I'll certainly let (at least some) jars finish colonizing, Probably not going to let these go beyond that..but i guess time will tell.
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ChocyMilky
Budding Mycologist



Registered: 03/30/20
Posts: 31
Last seen: 1 year, 11 months
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: Forrester]
#27963175 - 09/22/22 07:39 PM (2 years, 3 months ago) |
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Thank you, I will. They are actually not slimy..its not your typical, and quite obvious, bacteria. It's actually why I am opening it up to discussion. I hope someone more experienced looks at these pictures and says ahh yes this is (likely) exactly whats going on here.
There is "yellow" tinge grains and general discoloration, basically the only thing obvious to me is its not healthy mycelium.. I don't know what type of bacteria this is or yeast or more than one thing, nor am i certain where it's coming from. I'm leaning towards its in the LC for the reasons stated in op, additionally the mycelium that grows away from initial LC is bright white and healthy which tells me the contam doesn't appear to be originating from within the grain.
I'll grow it out and look at the whole jar and watch the newest mycelium and grain compared to the initial LC starting point mycelium/grain, and see what that tells me.
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ChocyMilky
Budding Mycologist



Registered: 03/30/20
Posts: 31
Last seen: 1 year, 11 months
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: bigfootscreepyuncl]
#27963177 - 09/22/22 07:39 PM (2 years, 3 months ago) |
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Thank you. I do.
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ChocyMilky
Budding Mycologist



Registered: 03/30/20
Posts: 31
Last seen: 1 year, 11 months
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: fiddle_head]
#27963214 - 09/22/22 07:51 PM (2 years, 3 months ago) |
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Quote:
fiddle_head said: Those grains look bacterial for a number of reasons. You can only wait it out but my guess chances are you’ll know if it’s trash before it’s fully colonized.
You're of the opinion the grains themselves are bacterial? As in they weren't properly sterilized to begin with.
Definitely possible, my experience is telling me if they are it's not obvious. I don't think they are A, because the same jars knocked up with the same agar wedges for the LC's were healthy. B, those grain jars came from the same lot as these.
Also, in reference to the LC jars. Where the LC came to rest and started colonizing is where I see obvious contamination/unhealthy mycelium and where I am seeing these yellow/discolored grain. Where the mycelium has grown away from the LC starting point, that new growth is white, and all of its colonized grain is white and clean looking. So if the whole of the grain is contaminated I would expect to see evidence of that throughout I would think, and not just where the LC started.
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fiddle_head
fettucine embargo



Registered: 08/05/08
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: ChocyMilky]
#27963274 - 09/22/22 08:14 PM (2 years, 3 months ago) |
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Quote:
ChocyMilky said:
Quote:
fiddle_head said: Those grains look bacterial for a number of reasons. You can only wait it out but my guess chances are you’ll know if it’s trash before it’s fully colonized.
You're of the opinion the grains themselves are bacterial? As in they weren't properly sterilized to begin with.
Definitely possible, my experience is telling me if they are it's not obvious. I don't think they are A, because the same jars knocked up with the same agar wedges for the LC's were healthy. B, those grain jars came from the same lot as these.
Also, in reference to the LC jars. Where the LC came to rest and started colonizing is where I see obvious contamination/unhealthy mycelium and where I am seeing these yellow/discolored grain. Where the mycelium has grown away from the LC starting point, that new growth is white, and all of its colonized grain is white and clean looking. So if the whole of the grain is contaminated I would expect to see evidence of that throughout I would think, and not just where the LC started.
No, that’s not what I said. Uncolonized or partially uncolonized grains pushed up against the side of the glass like that is a sign of bacteria. Also the yellow staining is from metabolites your myc is excreting to combat competing microorganisms in its environment. That’s what it looks like to me. I never said the “whole of your grain is contaminated” or that you didn’t sterilize properly, but it looks like you have bacterial growth in that jar. Someone correct me please.
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,563
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: fiddle_head]
#27963335 - 09/22/22 08:32 PM (2 years, 3 months ago) |
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Quote:
The agar and prepared grain are Clean. I can say that definitively because i did a side by side knocking up grain with the agar wedges used for the LC.
Quote:
do u have pics of the other jars to compare?
Quote:
The 4 pictures are of 4 separate jars. There's a few dozen jars, they all look like these.
i understand im looking at 4 jars nocked with LC. i was asking if u had photos of the jars nocked with agar
-------------------- Confused? Well now you can!
HHG - cheapest way to start - how i roll
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Doodleguy
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Registered: 04/23/22
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: fiddle_head]
#28022279 - 10/29/22 12:18 AM (2 years, 2 months ago) |
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hey there, Im fairly new to this.
would you mind listing some of those reasons?.
i currently have a few 3lb bags that Im debating using or not. they do not smell bad nor is the growth ice cream looking. a few of my peers are just as stumped as I.
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asmr
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Re: Would you spawn this? Yellow/discolored colonized grain. Can you definitively say what this is from experience? [Re: Doodleguy]
#28022293 - 10/29/22 12:44 AM (2 years, 2 months ago) |
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