|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Glass Agar Dishes info needed
#28022266 - 10/28/22 11:59 PM (2 years, 2 months ago) |
|
|
So, I bought some glass (autoclavable) petri dishes off amazon. I wanted agar dishes I could reuse that I could sterilize in my PC.
What I got looks way off. The lids and bottoms don't match. There is a 2-3 mm gap difference when you slide the bottoms all the way to a side. Is that normal for glass dishes?

Also... I have a vayepro hot plate. I have a glass stove and the manufacturer stated they did not recommend using a PC on it. The hot plate, turns on and off to maintain the desired heat setting, making pressure in the PC kind of irregular.
Anyone have any recommendations for a better one that would work with a 23 QT Presto?
|
HappyHigh
Stranger

Registered: 05/05/17
Posts: 905
Last seen: 3 months, 25 days
|
Re: Glass Agar Dishes info needed [Re: echochild]
#28022269 - 10/29/22 12:07 AM (2 years, 2 months ago) |
|
|
Mine are the same, I was sure to use tape around the whole edge of dish to keep it sturdy. I used in PC worked fine.
-------------------- Had I the heavens' embroidered cloths,
Enwrought with golden and silver light,
The blue and the dim and the dark cloths
Of night and light and the half light,
I would spread the cloths under your feet:
But I, being poor, have only my dreams;
I have spread my dreams under your feet;
Tread softly because you tread on my dreams.
|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Re: Glass Agar Dishes info needed [Re: HappyHigh]
#28022272 - 10/29/22 12:10 AM (2 years, 2 months ago) |
|
|
How are your contam rates with them? That seems like an awfully big gap...
|
HappyHigh
Stranger

Registered: 05/05/17
Posts: 905
Last seen: 3 months, 25 days
|
Re: Glass Agar Dishes info needed [Re: echochild] 2
#28022287 - 10/29/22 12:26 AM (2 years, 2 months ago) |
|
|
They have "parafilm" what most ppl use but it is a lil $$. I just use .3 micron tape 4$, round the outer edge then fold under too seal, stack fine. During PC I fold them in foil and leave it sealed till I use it.
-------------------- Had I the heavens' embroidered cloths,
Enwrought with golden and silver light,
The blue and the dim and the dark cloths
Of night and light and the half light,
I would spread the cloths under your feet:
But I, being poor, have only my dreams;
I have spread my dreams under your feet;
Tread softly because you tread on my dreams.
Edited by HappyHigh (10/29/22 12:27 AM)
|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Re: Glass Agar Dishes info needed [Re: HappyHigh]
#28022289 - 10/29/22 12:35 AM (2 years, 2 months ago) |
|
|
Yeah, I have parafilm. I tried micropore tape previously, but noticed I was having an unusually high rate of contam on those prepoured plates compared to the ones I was using parafilm with. Maybe I was just messing up my sterile technique, but as soon as I went back to parafilm, I stopped having the issue.
Thing is, the bottoms of the plates, and the bottom edges of the tops are pretty much flush with one another. I'm not sure that parafilm would grip that well, unless I'm doing several wraps with it (if even that would work?).
Maybe I will need to do another test with the micropore tape...
|
HappyHigh
Stranger

Registered: 05/05/17
Posts: 905
Last seen: 3 months, 25 days
|
Re: Glass Agar Dishes info needed [Re: echochild]
#28022299 - 10/29/22 12:53 AM (2 years, 2 months ago) |
|
|
Yeah I wondered the same about the parafilm if it would hold.
-------------------- Had I the heavens' embroidered cloths,
Enwrought with golden and silver light,
The blue and the dim and the dark cloths
Of night and light and the half light,
I would spread the cloths under your feet:
But I, being poor, have only my dreams;
I have spread my dreams under your feet;
Tread softly because you tread on my dreams.
|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Re: Glass Agar Dishes info needed [Re: HappyHigh]
#28022301 - 10/29/22 12:59 AM (2 years, 2 months ago) |
|
|
My crystal ball is showing me I have experimentation in my future
|
Toasted
Stranger
Registered: 05/24/22
Posts: 37
Last seen: 9 months, 22 days
|
Re: Glass Agar Dishes info needed [Re: echochild]
#28022305 - 10/29/22 01:09 AM (2 years, 2 months ago) |
|
|
The gaps are fine but more expensive petris do have less of a gap
Make sure edges are dry so your parafilm or saran wrap doesn't slip off
|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Re: Glass Agar Dishes info needed [Re: Toasted]
#28022306 - 10/29/22 01:13 AM (2 years, 2 months ago) |
|
|
Quote:
Toasted said: The gaps are fine but more expensive petris do have less of a gap
Make sure edges are dry so your parafilm or saran wrap doesn't slip off
Saran wrap.
Man... I need to go to bed. I've been PC'ing PF Tek pucks and was having issues with my circuit breaker deciding to be an asshole every time I would get close to getting ready to pressurize.
Saran wrap would indeed do the job and not obstruct my view of the plates. Now I just need to give them all a thorough look over. I noticed a crack in one of them.
Thank you all
|
bongoman
Stranger

Registered: 09/19/18
Posts: 579
Last seen: 2 days, 5 hours
|
Re: Glass Agar Dishes info needed [Re: echochild] 1
#28026490 - 10/31/22 04:37 PM (2 years, 2 months ago) |
|
|
I'v been using grafting tape with my glass petris.
But I notice that the extra weight of the glass material tends to put more pressure on the wrap when you are handling the plates and the wrap potentially can work itself loose.
I wonder if lab parafilm holds better?
|
Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
|
Re: Glass Agar Dishes info needed [Re: bongoman]
#28026497 - 10/31/22 04:38 PM (2 years, 2 months ago) |
|
|
Parafilm is great, but expensive compared to saran. If money didn't matter, I would go with parafilm ^^
|
kirkeng
Stranger


Registered: 02/14/21
Posts: 1,298
Loc: Lost
|
Re: Glass Agar Dishes info needed [Re: Shrimps] 1
#28026956 - 10/31/22 08:54 PM (2 years, 2 months ago) |
|
|
If you don’t want to do pre-pours with glass plates I recommend sterilizing clean dishes in the oven 1 hour at 400F does the trick, stacks wrapped in foil. Make sure they are clean and devoid of debris or sticky stuff if you use tape. Pouring glass dishes can also be cumbersome I pour in stacks of 5 because of this. Clean your dishes sooner rather than later when your done with them. If you wait to long you may need to scrub, if cleaned in good time you can just rinse them with water and dry them before sterilizing again. If you live by a university it’s worthwhile to contact them if they have a reuse program, I’ve gotten hundreds of old plates at .25-.5 cents a piece, when they out with the old and in with the new.
|
Suckatshrooms
Seriously, don’t listen to me.


Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
|
Re: Glass Agar Dishes info needed [Re: echochild]
#28026971 - 10/31/22 09:01 PM (2 years, 2 months ago) |
|
|
Quote:
echochild said: So, I bought some glass (autoclavable) petri dishes off amazon. I wanted agar dishes I could reuse that I could sterilize in my PC.
What I got looks way off. The lids and bottoms don't match. There is a 2-3 mm gap difference when you slide the bottoms all the way to a side. Is that normal for glass dishes?

Also... I have a vayepro hot plate. I have a glass stove and the manufacturer stated they did not recommend using a PC on it. The hot plate, turns on and off to maintain the desired heat setting, making pressure in the PC kind of irregular.
Anyone have any recommendations for a better one that would work with a 23 QT Presto?
Send them back and get Pyrex brand if you’re after quality. They’re perfect. If budget is a concern, I would go the opposite direction and get dirt cheap, non borosilicate glass. You can still pre pour and autoclave, but to be safe, let the PC cool to room temp before you open it.
|
Suckatshrooms
Seriously, don’t listen to me.


Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
|
Re: Glass Agar Dishes info needed [Re: bongoman]
#28026977 - 10/31/22 09:04 PM (2 years, 2 months ago) |
|
|
Quote:
bongoman said: I'v been using grafting tape with my glass petris.
But I notice that the extra weight of the glass material tends to put more pressure on the wrap when you are handling the plates and the wrap potentially can work itself loose.
I wonder if lab parafilm holds better?
Parafilm doesn’t budge on my glass plates.
|
Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 8 months, 22 days
|
Re: Glass Agar Dishes info needed [Re: echochild] 2
#28027014 - 10/31/22 09:24 PM (2 years, 2 months ago) |
|
|
Quote:
echochild said: How are your contam rates with them? That seems like an awfully big gap...
That's a terrible answer. Wrap the dish (like every petri dish, plastic or glass) with cling wrap or grafting tape and that gap now has 0 impact.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
We don't own things - things own us.
Semi-solid liquid culture (SSLC)
|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Re: Glass Agar Dishes info needed [Re: Rusty2096]
#28027097 - 10/31/22 10:22 PM (2 years, 2 months ago) |
|
|
Quote:
Rusty2096 said:
Quote:
echochild said: How are your contam rates with them? That seems like an awfully big gap...
That's a terrible answer. Wrap the dish (like every petri dish, plastic or glass) with cling wrap or grafting tape and that gap now has 0 impact.
Terrible question you mean? That was a question to a response.
Well, I PC'ed them yesterday and poured some agar. They are a bit cumbersome to work with. Use cling wrap to seal them, and they are in the waiting phase for a week to make sure no accidental contamination happened.
Before I PC'ed them I tested with parafilm. Considering the edges of the top are flush with the bottom, parafilm didn't play well, or I couldn't figure out how to make it play well like my other dishes, so I went with the wrap.
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,822
Last seen: 2 months, 5 days
|
Re: Glass Agar Dishes info needed [Re: echochild] 1
#28027133 - 10/31/22 10:54 PM (2 years, 2 months ago) |
|
|
hi! i use those glass dishes, the gap on the lid is totally normal. seems huge but is just how those cheaper glass dishes are. just use some cling wrap/seran wrap (same thing i just don’t know what everyone else called it). i cut my rolls about 1”-2” wide. you definitely want to make it wide enough to completely seal the dishes. i think parafilm would be too thin for my glass petri dishes, but i’ve never used parafilm so idk. i have a write up in my sig about using them for no pour too, if you’re sterilizing the plates in the PC it’s nicer to just no pour them imo but like a previous comment said they can just be oven sterilized if you’re trying to pour them. sandman420 has a good write up on that but it’s pretty straight forward
-------------------- you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================
 
🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
TheUsualSuspect
never tell me the odds


Registered: 09/11/22
Posts: 260
Last seen: 2 years, 5 days
|
Re: Glass Agar Dishes info needed [Re: hazyhorse]
#28027159 - 10/31/22 11:29 PM (2 years, 2 months ago) |
|
|
Out of curiosity, why the preference for glass pitris?
I use plastic ones pre sterilized. I like that they fit together tight and also the gap is in the center of the two so parafilm works fine (although I hate parafilm and am never convinced it 'stuck' where it was put). I also like that if I look in and its all nasty, I just chuck it in the trash without opening to expose myself to whatever is in there.
There must be benefits to glass, or is it just the plastic waste?
|
DERRAYLD
Constructus



Registered: 05/13/02
Posts: 12,101
Loc: South Africa
Last seen: 20 minutes, 11 seconds
|
|
Plastic waste, clarity and infinitely reusable, I also use pp cups but they too are not disposable. Disposable plates are incredibly wasteful and don't force you to focus in anyway.
|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Re: Glass Agar Dishes info needed [Re: DERRAYLD]
#28027211 - 11/01/22 12:47 AM (2 years, 2 months ago) |
|
|
Quote:
DERRAYLD said: Plastic waste, clarity and infinitely reusable, I also use pp cups but they too are not disposable. Disposable plates are incredibly wasteful and don't force you to focus in anyway.
Derrayld is on the nose. I'm not the most eco friendly person ever, but if I can, I do. I liked using ketchup cups, but hate how small they are and that they can't be reused.
Also... combined with making my own agar, a lot of money savings in the long run.
Hazy, thanks for giving me ONE MORE TAB to read... LOL joking. Thanks for the info, I will give it a read when I have more brains tomorrow.
|
DERRAYLD
Constructus



Registered: 05/13/02
Posts: 12,101
Loc: South Africa
Last seen: 20 minutes, 11 seconds
|
Re: Glass Agar Dishes info needed [Re: echochild]
#28027246 - 11/01/22 01:12 AM (2 years, 2 months ago) |
|
|
Quote:
echochild said:
Quote:
DERRAYLD said: Plastic waste, clarity and infinitely reusable, I also use pp cups but they too are not disposable. Disposable plates are incredibly wasteful and don't force you to focus in anyway.
Derrayld is on the nose. I'm not the most eco friendly person ever, but if I can, I do. I liked using ketchup cups, but hate how small they are and that they can't be reused.
Also... combined with making my own agar, a lot of money savings in the long run.
Hazy, thanks for giving me ONE MORE TAB to read... LOL joking. Thanks for the info, I will give it a read when I have more brains tomorrow.
Are you using pp ketchup cups? They are reusable.
|
echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
|
Re: Glass Agar Dishes info needed [Re: DERRAYLD] 1
#28027569 - 11/01/22 09:04 AM (2 years, 2 months ago) |
|
|
Quote:
DERRAYLD said:
Are you using pp ketchup cups? They are reusable.
I'm like 99.99% sure my on not PP: Diamond Multi-Purpose 50 Count Mini Cups with Lids, 2 oz, Clear.
They worked really well, but they are tiny and I would prefer to see the myc grow out just a little further. A little gripe, but I figure if I'm slowly upgrading my tech, I should start with the agar dishes.
|
Suckatshrooms
Seriously, don’t listen to me.


Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
|
|
Quote:
TheUsualSuspect said: Out of curiosity, why the preference for glass pitris?
I use plastic ones pre sterilized. I like that they fit together tight and also the gap is in the center of the two so parafilm works fine (although I hate parafilm and am never convinced it 'stuck' where it was put). I also like that if I look in and its all nasty, I just chuck it in the trash without opening to expose myself to whatever is in there.
There must be benefits to glass, or is it just the plastic waste?
I don’t care about plastic waste and glass is more expensive after I consider the cost of my time to reclean & sterilize. I use them for two reasons. 1) I keep accidentally breaking the plastic ones. The glass is way tougher and forgiving for me. 2) I can prepour agar before PCing so I don’t have to worry about a careful pour in my SAB.
Edited by Suckatshrooms (11/01/22 10:22 AM)
|
unicorngirl


Registered: 10/18/22
Posts: 13
Last seen: 2 years, 6 days
|
Re: Glass Agar Dishes info needed [Re: echochild] 1
#28028895 - 11/02/22 02:25 AM (2 years, 2 months ago) |
|
|
Hi echochild
Here is a pic of my nice pyrex dish:
 
The gap as 3mm.
So you'll be fine! Wooooohooooo haha
My dishes are 15mm tall, and two wraps of 1.5"-wide saran works well.
I also use this same roll to place an X on the bottom of each dish before sterilizing a foiled stack in the PC. This X prevents the dishes from sliding around and I leave it on throughout the lifecycle of the dish. I also place strips of saran-wrap on my worksurface. My stacks were literally blowing around in the flow and I lost a couple dishes learning how to work with them my first time So far, this technique works wonderfully, providing the needed 2-d friction when using 100mm wide glass petris. And I never have to worry,think, or deal with it again when handling the dishes.
I also am not strong or coordinated enough to safely pour up the stack from the bottom dish to the top when using glass petris. So I use an alternate (although slower) approach to protect my pretty dishes I take the top dish in the stack and start a new stack. Then I pour. Essentially, each dish is transferred to the new stack, then poured.
Other than working with stack heights that comfortably fit the hand, I can't think of anything else to contribute. I really enjoy working with glass petris and I hope you do tooooo
|
DERRAYLD
Constructus



Registered: 05/13/02
Posts: 12,101
Loc: South Africa
Last seen: 20 minutes, 11 seconds
|
|
Quote:
Suckatshrooms said:
Quote:
TheUsualSuspect said: Out of curiosity, why the preference for glass pitris?
I use plastic ones pre sterilized. I like that they fit together tight and also the gap is in the center of the two so parafilm works fine (although I hate parafilm and am never convinced it 'stuck' where it was put). I also like that if I look in and its all nasty, I just chuck it in the trash without opening to expose myself to whatever is in there.
There must be benefits to glass, or is it just the plastic waste?
I don’t care about plastic waste and glass is more expensive after I consider the cost of my time to reclean & sterilize. I use them for two reasons. 1) I keep accidentally breaking the plastic ones. The glass is way tougher and forgiving for me. 2) I can prepour agar before PCing so I don’t have to worry about a careful pour in my SAB.
Not to start something but you really should care about plastic waste.
|
unicorngirl


Registered: 10/18/22
Posts: 13
Last seen: 2 years, 6 days
|
Re: Glass Agar Dishes info needed [Re: unicorngirl] 1
#28028900 - 11/02/22 02:40 AM (2 years, 2 months ago) |
|
|
Oh, and regarding the pressure plate, the Cadco 3T works well for my large pressure cooker. It has alot of good reviews. It cycles on and off like yours and dial's in easily to maintain a steady pressure.
|
TheUsualSuspect
never tell me the odds


Registered: 09/11/22
Posts: 260
Last seen: 2 years, 5 days
|
Re: Glass Agar Dishes info needed [Re: DERRAYLD]
#28028909 - 11/02/22 02:57 AM (2 years, 2 months ago) |
|
|
Quote:
DERRAYLD said:
Quote:
Suckatshrooms said:
I use them for two reasons.
Not to start something but you really should care about plastic waste.
you say 'not to start something' but then go on to start something.... with someone who uses glass petris
|
DERRAYLD
Constructus



Registered: 05/13/02
Posts: 12,101
Loc: South Africa
Last seen: 20 minutes, 11 seconds
|
|
We can fight if you want, destruction of our planet is something I have no issue arguing about.
Come at me.
|
Pickle Rick
Gherkin



Registered: 07/24/22
Posts: 866
Loc: Jar
|
Re: Glass Agar Dishes info needed [Re: DERRAYLD]
#28028922 - 11/02/22 03:21 AM (2 years, 2 months ago) |
|
|
Quote:
echochild said: Is that normal for glass dishes?
Thats how all glass dishes are, even the pyrex ones.

The only time I have had any contam issues is when I first got them, I tried to set them upside down, like plastic dishes - due to the larger gap, that is not a suitable technique. After pouring, I just leave them in my SAB until I'm ready to use them. I only close them up with parafilm once I've used them.
re: your heating plate, dunno if its incompatible with pc's, I don't want to give you bad advice, but as far as I know thats how all electric heating plates work... they reach a certain temp, then the thermostat turns off the heating unit, it then cools to a certain temp, then turns on again.. and so forth.
Quote:
bongoman said: I'v been using grafting tape, I notice that the extra weight of the glass material tends to put more pressure on the wrap when you are handling the plates and the wrap potentially can work itself loose.
I wonder if lab parafilm holds better?
I've used grafting tape with plastic plates had no issues, haven't tried it on my glass ones, been using parafilm, which is indeed quite expensive. Might try cling film after this post.
Quote:
DERRAYLD said: Plastic waste, clarity and infinitely reusable, I also use pp cups but they too are not disposable. Disposable plates are incredibly wasteful and don't force you to focus in anyway.
That's originally why I opted for glass plates, however sterilizing them requires wrapping them in sealed foil which is also quite the pollutant, not really sure which is the lesser evil tbh
-------------------- P i c k l e R i c k
Pick Rick's Mushroom Chocolate
|
TheUsualSuspect
never tell me the odds


Registered: 09/11/22
Posts: 260
Last seen: 2 years, 5 days
|
Re: Glass Agar Dishes info needed [Re: DERRAYLD]
#28028923 - 11/02/22 03:22 AM (2 years, 2 months ago) |
|
|
You want to 'word fight' a stranger on the internet on a topic with movable goalposts in order to prove to a crowd of unknown people you are more socially relevant and worthy than they are.
I'm not really understanding that, but if you really want to...
glass is tha poop - plastic is tha best! down with your team - up with my team! you cant win - I will win! you are not win! it was a setup - that turtle snorted coke! trump said the best plastic!
am I doing it right?
|
DERRAYLD
Constructus



Registered: 05/13/02
Posts: 12,101
Loc: South Africa
Last seen: 20 minutes, 11 seconds
|
|

Just move along dude, we don't need to argue.
|
TheUsualSuspect
never tell me the odds


Registered: 09/11/22
Posts: 260
Last seen: 2 years, 5 days
|
Re: Glass Agar Dishes info needed [Re: DERRAYLD]
#28028926 - 11/02/22 03:25 AM (2 years, 2 months ago) |
|
|
Quote:
DERRAYLD said:
Come at me.
just sayin...
|
|